Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0623 and Psyr_0624 are separated by 353 nucleotides Psyr_0624 and Psyr_0625 are separated by 110 nucleotides Psyr_0625 and Psyr_0626 are separated by 3 nucleotides
Psyr_0623: Psyr_0623 - Peptidase S24, S26A and S26B, at 712,693 to 713,424
_0623
Psyr_0624: Psyr_0624 - Inorganic diphosphatase, at 713,778 to 714,305
_0624
Psyr_0625: Psyr_0625 - Protein of unknown function DUF980, at 714,416 to 715,225
_0625
Psyr_0626: Psyr_0626 - DedA, at 715,229 to 715,876
_0626
Position (kb) 713
714
715 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.515 kb on + strand at 713.537 kb on + strand at 713.537 kb on + strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.553 kb on + strand at 713.577 kb on + strand at 713.577 kb on + strand at 713.578 kb on - strand at 713.578 kb on - strand at 713.578 kb on - strand at 713.631 kb on + strand at 713.710 kb on + strand at 714.356 kb on - strand at 714.373 kb on - strand at 714.373 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand at 714.483 kb on + strand at 714.504 kb on + strand, within Psyr_0625 at 714.505 kb on - strand, within Psyr_0625 at 714.552 kb on + strand, within Psyr_0625 at 714.552 kb on + strand, within Psyr_0625 at 714.553 kb on - strand, within Psyr_0625 at 714.597 kb on + strand, within Psyr_0625 at 714.598 kb on - strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.773 kb on + strand, within Psyr_0625 at 714.774 kb on - strand, within Psyr_0625 at 714.774 kb on - strand, within Psyr_0625 at 714.774 kb on - strand, within Psyr_0625 at 714.774 kb on - strand, within Psyr_0625 at 714.780 kb on + strand, within Psyr_0625 at 714.780 kb on + strand, within Psyr_0625 at 714.781 kb on - strand, within Psyr_0625 at 714.806 kb on + strand, within Psyr_0625 at 715.133 kb on - strand, within Psyr_0625 at 715.160 kb on + strand at 715.160 kb on + strand at 715.160 kb on + strand at 715.268 kb on + strand at 715.268 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 712,901 + Psyr_0623 0.28 -1.3 712,901 + Psyr_0623 0.28 +0.3 712,901 + Psyr_0623 0.28 +0.2 712,901 + Psyr_0623 0.28 -1.6 712,901 + Psyr_0623 0.28 +0.5 713,418 + -0.1 713,418 + +1.1 713,418 + -0.1 713,418 + +1.1 713,418 + -1.0 713,418 + -0.2 713,418 + -1.6 713,418 + -2.1 713,418 + +0.1 713,418 + +0.6 713,418 + -0.3 713,418 + -1.9 713,418 + -0.1 713,418 + +0.1 713,418 + +0.1 713,418 + +0.6 713,418 + -0.6 713,418 + -2.0 713,418 + +0.5 713,418 + -0.2 713,418 + -0.0 713,419 - -1.5 713,419 - -2.7 713,419 - +0.8 713,419 - -0.5 713,419 - +0.7 713,419 - -0.8 713,419 - -0.4 713,419 - -1.4 713,419 - +0.6 713,419 - -0.6 713,419 - +1.1 713,419 - -0.2 713,419 - -0.3 713,419 - -1.9 713,419 - -0.5 713,419 - -0.4 713,419 - -0.2 713,419 - -0.1 713,419 - +3.0 713,419 - +1.1 713,419 - +0.2 713,419 - -0.2 713,419 - -0.3 713,419 - -2.9 713,419 - +0.8 713,458 + -0.1 713,458 + -1.7 713,458 + +0.7 713,458 + +0.6 713,458 + +0.2 713,515 + +0.2 713,537 + +0.5 713,537 + -1.0 713,538 - +1.0 713,538 - -0.1 713,538 - +0.3 713,538 - +0.4 713,538 - -1.4 713,553 + -0.1 713,577 + -0.4 713,577 + +0.4 713,578 - +0.4 713,578 - -1.0 713,578 - +1.4 713,631 + -1.7 713,710 + +0.4 714,356 - +0.3 714,373 - +0.2 714,373 - -0.2 714,379 - -0.1 714,379 - -1.6 714,379 - +1.6 714,379 - -0.8 714,379 - -0.1 714,483 + -0.7 714,504 + Psyr_0625 0.11 -0.1 714,505 - Psyr_0625 0.11 +2.0 714,552 + Psyr_0625 0.17 +1.0 714,552 + Psyr_0625 0.17 +0.1 714,553 - Psyr_0625 0.17 +1.4 714,597 + Psyr_0625 0.22 -0.9 714,598 - Psyr_0625 0.22 +0.4 714,773 + Psyr_0625 0.44 +0.5 714,773 + Psyr_0625 0.44 -1.0 714,773 + Psyr_0625 0.44 +1.6 714,773 + Psyr_0625 0.44 -0.4 714,773 + Psyr_0625 0.44 +0.4 714,773 + Psyr_0625 0.44 -1.7 714,773 + Psyr_0625 0.44 +0.8 714,773 + Psyr_0625 0.44 -0.8 714,774 - Psyr_0625 0.44 -1.7 714,774 - Psyr_0625 0.44 +0.4 714,774 - Psyr_0625 0.44 -0.1 714,774 - Psyr_0625 0.44 +0.4 714,780 + Psyr_0625 0.45 +0.5 714,780 + Psyr_0625 0.45 +0.0 714,781 - Psyr_0625 0.45 -0.0 714,806 + Psyr_0625 0.48 -0.2 715,133 - Psyr_0625 0.89 -0.0 715,160 + +0.7 715,160 + +0.5 715,160 + +0.0 715,268 + -0.5 715,268 + +1.0
Or see this region's nucleotide sequence