Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0580 and Psyr_0581 are separated by 257 nucleotides Psyr_0581 and Psyr_0582 are separated by 28 nucleotides Psyr_0582 and Psyr_0583 are separated by 39 nucleotides
Psyr_0580: Psyr_0580 - 23S rRNA Gm-2251 2'-O-methyltransferase, at 662,568 to 663,323
_0580
Psyr_0581: Psyr_0581 - SSU ribosomal protein S6P, at 663,581 to 664,006
_0581
Psyr_0582: Psyr_0582 - SSU ribosomal protein S18P, at 664,035 to 664,265
_0582
Psyr_0583: Psyr_0583 - membrane protein, putative, at 664,305 to 665,201
_0583
Position (kb) 663
664
665 Strain fitness (log2 ratio) -2
-1
0
1
2 at 662.605 kb on + strand at 662.605 kb on + strand at 662.606 kb on - strand at 662.614 kb on + strand at 662.614 kb on + strand at 662.614 kb on + strand at 662.615 kb on - strand at 662.915 kb on + strand, within Psyr_0580 at 662.915 kb on + strand, within Psyr_0580 at 663.051 kb on - strand, within Psyr_0580 at 663.121 kb on - strand, within Psyr_0580 at 663.152 kb on + strand, within Psyr_0580 at 663.207 kb on + strand, within Psyr_0580 at 663.208 kb on - strand, within Psyr_0580 at 663.321 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.376 kb on + strand at 663.376 kb on + strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.382 kb on + strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.421 kb on + strand at 663.422 kb on - strand at 663.422 kb on - strand at 664.614 kb on + strand, within Psyr_0583 at 664.614 kb on + strand, within Psyr_0583 at 664.614 kb on + strand, within Psyr_0583 at 664.621 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.964 kb on + strand, within Psyr_0583 at 664.964 kb on + strand, within Psyr_0583
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 662,605 + -0.1 662,605 + -0.0 662,606 - +0.0 662,614 + -0.3 662,614 + -0.1 662,614 + +0.3 662,615 - +0.1 662,915 + Psyr_0580 0.46 -1.5 662,915 + Psyr_0580 0.46 -0.4 663,051 - Psyr_0580 0.64 -0.7 663,121 - Psyr_0580 0.73 -1.3 663,152 + Psyr_0580 0.77 +0.4 663,207 + Psyr_0580 0.85 +0.7 663,208 - Psyr_0580 0.85 -1.9 663,321 + +0.1 663,324 + -0.0 663,324 + +1.0 663,324 + +0.2 663,324 + -1.0 663,352 + -1.3 663,352 + -1.1 663,352 + -0.3 663,352 + +0.5 663,352 + -1.1 663,352 + -0.9 663,353 - +0.1 663,353 - +0.8 663,353 - -1.7 663,353 - +1.2 663,359 + +0.3 663,359 + -0.8 663,359 + -0.5 663,359 + -0.7 663,360 - +0.9 663,360 - -0.1 663,360 - +0.1 663,360 - +0.9 663,360 - +2.2 663,360 - +0.6 663,360 - +0.6 663,376 + +0.5 663,376 + +0.4 663,377 - -2.2 663,377 - -0.9 663,377 - +1.1 663,377 - +0.1 663,377 - -0.4 663,382 + -0.5 663,383 - -0.0 663,383 - -0.1 663,421 + -0.1 663,422 - -0.1 663,422 - -0.3 664,614 + Psyr_0583 0.34 -0.7 664,614 + Psyr_0583 0.34 +1.0 664,614 + Psyr_0583 0.34 +0.8 664,621 + Psyr_0583 0.35 -2.2 664,723 + Psyr_0583 0.47 +1.0 664,723 + Psyr_0583 0.47 -1.4 664,723 + Psyr_0583 0.47 +0.7 664,845 + Psyr_0583 0.60 +0.9 664,845 + Psyr_0583 0.60 -2.2 664,845 + Psyr_0583 0.60 -0.1 664,845 + Psyr_0583 0.60 -0.4 664,845 + Psyr_0583 0.60 +1.2 664,845 + Psyr_0583 0.60 +2.0 664,964 + Psyr_0583 0.73 +0.0 664,964 + Psyr_0583 0.73 -0.1
Or see this region's nucleotide sequence