Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0235 and Psyr_0236 overlap by 29 nucleotides Psyr_0236 and Psyr_0237 are separated by 74 nucleotides
Psyr_0235: Psyr_0235 - D-amino acid dehydrogenase small subunit, at 249,112 to 250,413
_0235
Psyr_0236: Psyr_0236 - Endoribonuclease L-PSP, at 250,385 to 250,738
_0236
Psyr_0237: Psyr_0237 - alanine racemase, at 250,813 to 251,886
_0237
Position (kb) 250
251 Strain fitness (log2 ratio) -2
-1
0
1 at 249.412 kb on + strand, within Psyr_0235 at 249.601 kb on + strand, within Psyr_0235 at 249.602 kb on - strand, within Psyr_0235 at 249.813 kb on + strand, within Psyr_0235 at 249.857 kb on - strand, within Psyr_0235 at 249.860 kb on + strand, within Psyr_0235 at 249.860 kb on + strand, within Psyr_0235 at 249.922 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.198 kb on - strand, within Psyr_0235 at 250.347 kb on + strand at 250.347 kb on + strand at 250.347 kb on + strand at 250.347 kb on + strand at 250.347 kb on + strand at 250.348 kb on - strand at 250.348 kb on - strand at 250.348 kb on - strand at 250.365 kb on + strand at 250.451 kb on + strand, within Psyr_0236 at 250.452 kb on - strand, within Psyr_0236 at 250.802 kb on + strand at 250.802 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 249,412 + Psyr_0235 0.23 +0.2 249,601 + Psyr_0235 0.38 -0.7 249,602 - Psyr_0235 0.38 -0.0 249,813 + Psyr_0235 0.54 +0.3 249,857 - Psyr_0235 0.57 -1.4 249,860 + Psyr_0235 0.57 -1.2 249,860 + Psyr_0235 0.57 -0.8 249,922 + Psyr_0235 0.62 -0.6 250,003 + Psyr_0235 0.68 -0.4 250,003 + Psyr_0235 0.68 -1.1 250,003 + Psyr_0235 0.68 +0.7 250,003 + Psyr_0235 0.68 -1.0 250,003 + Psyr_0235 0.68 -0.2 250,004 - Psyr_0235 0.69 -1.4 250,004 - Psyr_0235 0.69 -1.3 250,004 - Psyr_0235 0.69 -0.8 250,004 - Psyr_0235 0.69 -0.9 250,198 - Psyr_0235 0.83 -0.8 250,347 + -0.9 250,347 + -0.1 250,347 + -0.3 250,347 + +1.3 250,347 + -0.5 250,348 - +1.6 250,348 - -1.8 250,348 - -1.0 250,365 + +0.8 250,451 + Psyr_0236 0.19 +1.2 250,452 - Psyr_0236 0.19 -0.3 250,802 + -1.0 250,802 + -0.3
Or see this region's nucleotide sequence