Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0220 and Psyr_0221 are separated by 6 nucleotides Psyr_0221 and Psyr_0222 are separated by 96 nucleotides Psyr_0222 and Psyr_0223 are separated by 133 nucleotides
Psyr_0220: Psyr_0220 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 234,646 to 235,101
_0220
Psyr_0221: Psyr_0221 - Phosphopantothenate-cysteine ligase / Phosphopantothenoylcysteine decarboxylase, at 235,108 to 236,355
_0221
Psyr_0222: Psyr_0222 - DNA replication and repair protein RadC, at 236,452 to 237,126
_0222
Psyr_0223: Psyr_0223 - extracellular solute-binding protein, family 5, at 237,260 to 238,846
_0223
Position (kb)
235
236
237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 234.585 kb on + strand at 234.586 kb on - strand at 234.586 kb on - strand at 234.629 kb on + strand at 234.629 kb on + strand at 234.629 kb on + strand at 234.630 kb on - strand at 234.630 kb on - strand at 234.630 kb on - strand at 234.630 kb on - strand at 234.630 kb on - strand at 234.630 kb on - strand at 234.630 kb on - strand at 234.630 kb on - strand at 235.106 kb on - strand at 235.106 kb on - strand at 235.110 kb on - strand at 235.110 kb on - strand at 235.110 kb on - strand at 236.403 kb on - strand at 236.451 kb on + strand at 236.451 kb on + strand at 236.451 kb on + strand at 236.452 kb on - strand at 236.452 kb on - strand at 236.452 kb on - strand at 236.452 kb on - strand at 236.457 kb on + strand at 236.582 kb on + strand, within Psyr_0222 at 236.583 kb on - strand, within Psyr_0222 at 236.583 kb on - strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.785 kb on + strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.873 kb on + strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 237.124 kb on + strand at 237.124 kb on + strand at 237.124 kb on + strand at 237.124 kb on + strand at 237.125 kb on - strand at 237.125 kb on - strand at 237.143 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 234,585 + +1.6 234,586 - -0.5 234,586 - +0.2 234,629 + +0.1 234,629 + +0.1 234,629 + +0.3 234,630 - -0.8 234,630 - -0.1 234,630 - -0.1 234,630 - -0.3 234,630 - +0.4 234,630 - -0.4 234,630 - -0.4 234,630 - +0.2 235,106 - -0.3 235,106 - -1.0 235,110 - -0.4 235,110 - +0.1 235,110 - -0.0 236,403 - +0.2 236,451 + +0.3 236,451 + +0.2 236,451 + +0.9 236,452 - +0.3 236,452 - +0.5 236,452 - -0.4 236,452 - +0.3 236,457 + -0.2 236,582 + Psyr_0222 0.19 +0.2 236,583 - Psyr_0222 0.19 +1.0 236,583 - Psyr_0222 0.19 +0.5 236,785 + Psyr_0222 0.49 +0.2 236,785 + Psyr_0222 0.49 -2.7 236,785 + Psyr_0222 0.49 +0.0 236,785 + Psyr_0222 0.49 -0.6 236,785 + Psyr_0222 0.49 +0.9 236,786 - Psyr_0222 0.49 +0.2 236,786 - Psyr_0222 0.49 +1.0 236,786 - Psyr_0222 0.49 -1.1 236,786 - Psyr_0222 0.49 +0.2 236,873 + Psyr_0222 0.62 +0.4 236,874 - Psyr_0222 0.63 +0.8 236,874 - Psyr_0222 0.63 -0.4 236,874 - Psyr_0222 0.63 -0.2 236,874 - Psyr_0222 0.63 -0.2 236,874 - Psyr_0222 0.63 -0.4 236,902 + Psyr_0222 0.67 -0.1 236,902 + Psyr_0222 0.67 +0.7 236,902 + Psyr_0222 0.67 -0.1 236,902 + Psyr_0222 0.67 +0.3 236,902 + Psyr_0222 0.67 -0.1 236,902 + Psyr_0222 0.67 -2.3 236,902 + Psyr_0222 0.67 +0.6 236,902 + Psyr_0222 0.67 -0.6 236,902 + Psyr_0222 0.67 +0.4 236,902 + Psyr_0222 0.67 +0.8 236,902 + Psyr_0222 0.67 +0.4 236,902 + Psyr_0222 0.67 +0.3 236,903 - Psyr_0222 0.67 -0.6 236,903 - Psyr_0222 0.67 -0.1 236,903 - Psyr_0222 0.67 +0.9 236,903 - Psyr_0222 0.67 -0.8 236,903 - Psyr_0222 0.67 +1.7 236,903 - Psyr_0222 0.67 -2.2 236,903 - Psyr_0222 0.67 +0.3 236,903 - Psyr_0222 0.67 +0.1 236,903 - Psyr_0222 0.67 +0.7 236,903 - Psyr_0222 0.67 -0.0 236,903 - Psyr_0222 0.67 -0.3 236,903 - Psyr_0222 0.67 +0.9 236,906 + Psyr_0222 0.67 -0.0 236,906 + Psyr_0222 0.67 -0.5 236,906 + Psyr_0222 0.67 +0.7 236,906 + Psyr_0222 0.67 +0.0 236,906 + Psyr_0222 0.67 -0.1 236,906 + Psyr_0222 0.67 -0.7 236,906 + Psyr_0222 0.67 -0.5 236,907 - Psyr_0222 0.67 +1.3 236,907 - Psyr_0222 0.67 +2.6 236,907 - Psyr_0222 0.67 -0.2 236,907 - Psyr_0222 0.67 -0.0 236,907 - Psyr_0222 0.67 -0.2 236,907 - Psyr_0222 0.67 -0.6 236,907 - Psyr_0222 0.67 -0.9 236,907 - Psyr_0222 0.67 -1.8 236,907 - Psyr_0222 0.67 -0.0 236,907 - Psyr_0222 0.67 -0.3 237,124 + +0.3 237,124 + +0.2 237,124 + -1.2 237,124 + -0.6 237,125 - -1.3 237,125 - +1.2 237,143 + -0.3 237,261 + +0.4 237,261 + -1.0 237,261 + -0.5 237,261 + -1.2 237,261 + +0.7 237,261 + +0.6 237,261 + -3.0 237,261 + -0.2 237,261 + +1.2 237,261 + +0.6 237,262 - +0.5 237,262 - +1.2 237,262 - +0.3 237,262 - +0.9 237,262 - -0.3 237,280 - -0.8
Or see this region's nucleotide sequence