Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0214 and Psyr_0215 are separated by 69 nucleotides Psyr_0215 and Psyr_0216 are separated by 82 nucleotides Psyr_0216 and Psyr_0217 are separated by 10 nucleotides Psyr_0217 and Psyr_0218 are separated by 331 nucleotides
Psyr_0214: Psyr_0214 - conserved hypothetical protein, at 228,159 to 228,527
_0214
Psyr_0215: Psyr_0215 - Exodeoxyribonuclease III xth, at 228,597 to 229,376
_0215
Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100
_0216
Psyr_0217: Psyr_0217 - conserved hypothetical protein, at 230,111 to 230,743
_0217
Psyr_0218: Psyr_0218 - N-acetylglutamate kinase, at 231,075 to 231,980
_0218
Position (kb)
229
230
231 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 228.470 kb on - strand, within Psyr_0214 at 228.492 kb on + strand at 228.492 kb on + strand at 228.492 kb on + strand at 228.493 kb on - strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.603 kb on - strand at 228.603 kb on - strand at 228.603 kb on - strand at 228.619 kb on + strand at 228.715 kb on + strand, within Psyr_0215 at 228.716 kb on - strand, within Psyr_0215 at 228.776 kb on - strand, within Psyr_0215 at 228.776 kb on - strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.943 kb on + strand, within Psyr_0215 at 228.943 kb on + strand, within Psyr_0215 at 228.943 kb on + strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.949 kb on + strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 229.166 kb on - strand, within Psyr_0215 at 229.166 kb on - strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.211 kb on - strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.353 kb on + strand at 229.353 kb on + strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.376 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.404 kb on - strand at 229.404 kb on - strand at 229.420 kb on - strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.427 kb on + strand at 229.427 kb on + strand at 229.428 kb on - strand at 229.442 kb on + strand at 229.672 kb on + strand, within Psyr_0216 at 230.238 kb on - strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.313 kb on - strand, within Psyr_0217 at 230.365 kb on + strand, within Psyr_0217 at 230.438 kb on + strand, within Psyr_0217 at 230.439 kb on - strand, within Psyr_0217 at 230.839 kb on + strand at 231.043 kb on - strand at 231.065 kb on + strand at 231.066 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 228,470 - Psyr_0214 0.84 -0.5 228,492 + -0.9 228,492 + -0.4 228,492 + +0.2 228,493 - +0.7 228,525 + +0.6 228,525 + -1.0 228,525 + +0.0 228,525 + +1.6 228,525 + -3.2 228,525 + +0.6 228,525 + +0.2 228,525 + -0.7 228,525 + +1.6 228,526 - +0.6 228,526 - +0.6 228,526 - +0.4 228,526 - +0.2 228,526 - -1.0 228,526 - +0.2 228,526 - -0.3 228,526 - -2.1 228,526 - -1.0 228,603 - -0.4 228,603 - +0.8 228,603 - +1.0 228,619 + +1.6 228,715 + Psyr_0215 0.15 -1.2 228,716 - Psyr_0215 0.15 -1.8 228,776 - Psyr_0215 0.23 -0.8 228,776 - Psyr_0215 0.23 +0.2 228,928 + Psyr_0215 0.42 -2.2 228,928 + Psyr_0215 0.42 -1.4 228,928 + Psyr_0215 0.42 -2.4 228,928 + Psyr_0215 0.42 -3.3 228,928 + Psyr_0215 0.42 +0.7 228,928 + Psyr_0215 0.42 -0.8 228,929 - Psyr_0215 0.43 -0.8 228,929 - Psyr_0215 0.43 +0.4 228,929 - Psyr_0215 0.43 -0.8 228,929 - Psyr_0215 0.43 -2.1 228,929 - Psyr_0215 0.43 -0.0 228,929 - Psyr_0215 0.43 -0.8 228,929 - Psyr_0215 0.43 +0.3 228,943 + Psyr_0215 0.44 -0.4 228,943 + Psyr_0215 0.44 -1.2 228,943 + Psyr_0215 0.44 -2.7 228,944 - Psyr_0215 0.44 -2.3 228,944 - Psyr_0215 0.44 -0.4 228,944 - Psyr_0215 0.44 -2.2 228,944 - Psyr_0215 0.44 -1.8 228,944 - Psyr_0215 0.44 -1.2 228,949 + Psyr_0215 0.45 +1.0 228,950 - Psyr_0215 0.45 -2.2 228,950 - Psyr_0215 0.45 -1.0 228,950 - Psyr_0215 0.45 -0.8 228,950 - Psyr_0215 0.45 -1.5 228,950 - Psyr_0215 0.45 -0.0 228,950 - Psyr_0215 0.45 -1.0 228,982 + Psyr_0215 0.49 -1.9 228,982 + Psyr_0215 0.49 -0.8 228,982 + Psyr_0215 0.49 -1.0 228,982 + Psyr_0215 0.49 -0.8 228,982 + Psyr_0215 0.49 +1.6 228,982 + Psyr_0215 0.49 -2.3 228,982 + Psyr_0215 0.49 -1.4 228,982 + Psyr_0215 0.49 -2.0 228,982 + Psyr_0215 0.49 -1.0 228,982 + Psyr_0215 0.49 +2.0 228,982 + Psyr_0215 0.49 -0.8 228,983 - Psyr_0215 0.49 -3.0 228,983 - Psyr_0215 0.49 -1.0 228,983 - Psyr_0215 0.49 -0.9 228,983 - Psyr_0215 0.49 +0.1 228,983 - Psyr_0215 0.49 -2.4 229,166 - Psyr_0215 0.73 -0.0 229,166 - Psyr_0215 0.73 -1.8 229,210 + Psyr_0215 0.79 -0.0 229,210 + Psyr_0215 0.79 -0.5 229,211 - Psyr_0215 0.79 -1.3 229,219 + Psyr_0215 0.80 -1.0 229,219 + Psyr_0215 0.80 -0.4 229,219 + Psyr_0215 0.80 -1.3 229,220 - Psyr_0215 0.80 -1.5 229,220 - Psyr_0215 0.80 -0.5 229,278 + Psyr_0215 0.87 -1.2 229,278 + Psyr_0215 0.87 -1.6 229,353 + +1.6 229,353 + -2.1 229,354 - -3.3 229,354 - -2.7 229,354 - -1.5 229,354 - -0.5 229,376 + -0.4 229,403 + -2.3 229,403 + -1.9 229,403 + -1.2 229,403 + -1.8 229,404 - -0.0 229,404 - +0.2 229,420 - -0.7 229,423 + -1.6 229,423 + -0.1 229,423 + -1.5 229,423 + +0.1 229,423 + +0.8 229,427 + -0.9 229,427 + -1.1 229,428 - -1.4 229,442 + -1.2 229,672 + Psyr_0216 0.33 -0.2 230,238 - Psyr_0217 0.20 +0.1 230,297 + Psyr_0217 0.29 -1.1 230,297 + Psyr_0217 0.29 -0.5 230,298 - Psyr_0217 0.30 -0.5 230,298 - Psyr_0217 0.30 -0.3 230,298 - Psyr_0217 0.30 -0.8 230,298 - Psyr_0217 0.30 -0.6 230,313 - Psyr_0217 0.32 +0.9 230,365 + Psyr_0217 0.40 -0.1 230,438 + Psyr_0217 0.52 +1.2 230,439 - Psyr_0217 0.52 +1.2 230,839 + +1.7 231,043 - -0.4 231,065 + +0.8 231,066 - +0.6
Or see this region's nucleotide sequence