Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0182 and Psyr_0183 are separated by 5 nucleotides Psyr_0183 and Psyr_0184 are separated by 13 nucleotides Psyr_0184 and Psyr_0185 are separated by 2 nucleotides
Psyr_0182: Psyr_0182 - diaminopimelate decarboxylase, at 195,060 to 196,307
_0182
Psyr_0183: Psyr_0183 - diaminopimelate epimerase, at 196,313 to 197,143
_0183
Psyr_0184: Psyr_0184 - Protein of unknown function DUF484, at 197,157 to 197,891
_0184
Psyr_0185: Psyr_0185 - tyrosine recombinase XerC subunit, at 197,894 to 198,793
_0185
Position (kb) 196
197
198 Strain fitness (log2 ratio) -2
-1
0
1
2 at 195.540 kb on + strand, within Psyr_0182 at 197.149 kb on + strand at 197.149 kb on + strand at 197.149 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.793 kb on + strand, within Psyr_0184 at 197.793 kb on + strand, within Psyr_0184 at 197.793 kb on + strand, within Psyr_0184 at 197.794 kb on - strand, within Psyr_0184 at 197.794 kb on - strand, within Psyr_0184 at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.826 kb on + strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.827 kb on - strand at 197.828 kb on + strand at 197.875 kb on + strand at 197.915 kb on + strand at 197.916 kb on - strand at 197.916 kb on - strand at 197.916 kb on - strand at 197.916 kb on - strand at 197.999 kb on + strand, within Psyr_0185 at 198.000 kb on - strand, within Psyr_0185 at 198.134 kb on + strand, within Psyr_0185 at 198.134 kb on + strand, within Psyr_0185 at 198.134 kb on + strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.135 kb on - strand, within Psyr_0185 at 198.140 kb on + strand, within Psyr_0185 at 198.140 kb on + strand, within Psyr_0185 at 198.140 kb on + strand, within Psyr_0185 at 198.141 kb on - strand, within Psyr_0185 at 198.141 kb on - strand, within Psyr_0185 at 198.141 kb on - strand, within Psyr_0185
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 195,540 + Psyr_0182 0.38 -0.4 197,149 + -2.1 197,149 + +0.5 197,149 + -1.1 197,153 + +0.6 197,153 + +0.6 197,153 + +0.4 197,153 + +0.1 197,154 - +0.4 197,154 - -0.7 197,154 - -0.2 197,154 - +0.1 197,154 - +0.5 197,154 - -0.5 197,154 - -0.4 197,154 - +0.2 197,793 + Psyr_0184 0.87 -0.4 197,793 + Psyr_0184 0.87 +0.7 197,793 + Psyr_0184 0.87 +0.4 197,794 - Psyr_0184 0.87 +0.2 197,794 - Psyr_0184 0.87 +0.9 197,826 + +0.6 197,826 + -2.0 197,826 + -0.4 197,826 + +0.9 197,826 + +0.3 197,826 + +0.4 197,827 - +0.4 197,827 - +0.6 197,827 - -0.1 197,827 - +0.4 197,827 - -0.5 197,827 - -2.4 197,827 - +0.5 197,827 - -0.1 197,827 - +2.5 197,828 + +0.8 197,875 + +0.8 197,915 + +1.0 197,916 - +0.7 197,916 - -1.5 197,916 - -0.1 197,916 - -0.0 197,999 + Psyr_0185 0.12 -0.0 198,000 - Psyr_0185 0.12 -0.8 198,134 + Psyr_0185 0.27 -0.7 198,134 + Psyr_0185 0.27 +0.6 198,134 + Psyr_0185 0.27 +0.1 198,135 - Psyr_0185 0.27 +1.6 198,135 - Psyr_0185 0.27 +0.3 198,135 - Psyr_0185 0.27 +1.7 198,135 - Psyr_0185 0.27 +0.8 198,135 - Psyr_0185 0.27 +0.0 198,135 - Psyr_0185 0.27 -0.6 198,135 - Psyr_0185 0.27 +1.5 198,140 + Psyr_0185 0.27 -0.7 198,140 + Psyr_0185 0.27 -1.8 198,140 + Psyr_0185 0.27 -0.6 198,141 - Psyr_0185 0.27 +0.3 198,141 - Psyr_0185 0.27 -2.2 198,141 - Psyr_0185 0.27 -0.6
Or see this region's nucleotide sequence