Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0179 and Psyr_0180 are separated by 2 nucleotides Psyr_0180 and Psyr_0181 are separated by 238 nucleotides Psyr_0181 and Psyr_0182 are separated by 9 nucleotides Psyr_0182 and Psyr_0183 are separated by 5 nucleotides Psyr_0183 and Psyr_0184 are separated by 13 nucleotides
Psyr_0179: Psyr_0179 - Protein of unknown function DUF1289, at 194,094 to 194,330
_0179
Psyr_0180: Psyr_0180 - Frataxin-like protein, at 194,333 to 194,665
_0180
Psyr_0181: Psyr_0181 - lipoprotein LppL, at 194,904 to 195,050
_0181
Psyr_0182: Psyr_0182 - diaminopimelate decarboxylase, at 195,060 to 196,307
_0182
Psyr_0183: Psyr_0183 - diaminopimelate epimerase, at 196,313 to 197,143
_0183
Psyr_0184: Psyr_0184 - Protein of unknown function DUF484, at 197,157 to 197,891
_0184
Position (kb) 195
196
197 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 194.071 kb on + strand at 194.072 kb on - strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.258 kb on + strand, within Psyr_0179 at 194.258 kb on + strand, within Psyr_0179 at 194.259 kb on - strand, within Psyr_0179 at 194.449 kb on - strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.553 kb on - strand, within Psyr_0180 at 194.600 kb on + strand, within Psyr_0180 at 194.600 kb on + strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.612 kb on - strand, within Psyr_0180 at 194.694 kb on + strand at 194.694 kb on + strand at 194.694 kb on + strand at 194.694 kb on + strand at 194.712 kb on + strand at 194.741 kb on + strand at 194.741 kb on + strand at 194.741 kb on + strand at 194.771 kb on - strand at 194.771 kb on - strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 195.540 kb on + strand, within Psyr_0182 at 197.149 kb on + strand at 197.149 kb on + strand at 197.149 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.153 kb on + strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand at 197.154 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 194,071 + -0.2 194,072 - +0.7 194,075 + -0.7 194,075 + -0.4 194,075 + -0.3 194,075 + +0.6 194,075 + +1.2 194,076 - +0.4 194,076 - -0.6 194,076 - +0.0 194,076 - -0.9 194,076 - -1.0 194,076 - +0.3 194,076 - +0.1 194,076 - +0.4 194,076 - +1.0 194,076 - -0.0 194,076 - +0.1 194,258 + Psyr_0179 0.69 -0.4 194,258 + Psyr_0179 0.69 -0.6 194,259 - Psyr_0179 0.70 +0.1 194,449 - Psyr_0180 0.35 -0.5 194,518 + Psyr_0180 0.56 -0.5 194,518 + Psyr_0180 0.56 -1.2 194,518 + Psyr_0180 0.56 -0.2 194,518 + Psyr_0180 0.56 -0.1 194,519 - Psyr_0180 0.56 +0.2 194,519 - Psyr_0180 0.56 +0.9 194,519 - Psyr_0180 0.56 +0.4 194,553 - Psyr_0180 0.66 -0.3 194,600 + Psyr_0180 0.80 -1.4 194,600 + Psyr_0180 0.80 -0.8 194,601 - Psyr_0180 0.80 -0.5 194,601 - Psyr_0180 0.80 +0.4 194,601 - Psyr_0180 0.80 -1.0 194,612 - Psyr_0180 0.84 -0.2 194,694 + -0.6 194,694 + -0.2 194,694 + +0.7 194,694 + -0.7 194,712 + -0.3 194,741 + +0.6 194,741 + +0.3 194,741 + -3.2 194,771 - -0.1 194,771 - +0.3 194,786 + +0.6 194,786 + -1.3 194,786 + -0.4 194,786 + +0.2 194,786 + -0.7 194,787 - -1.9 194,787 - +0.1 194,787 - +0.1 194,787 - -0.2 194,787 - -0.4 194,787 - +1.3 194,787 - -1.9 194,787 - -1.1 195,540 + Psyr_0182 0.38 -0.4 197,149 + -2.1 197,149 + +0.5 197,149 + -1.1 197,153 + +0.6 197,153 + +0.6 197,153 + +0.4 197,153 + +0.1 197,154 - +0.4 197,154 - -0.7 197,154 - -0.2 197,154 - +0.1 197,154 - +0.5 197,154 - -0.5 197,154 - -0.4 197,154 - +0.2
Or see this region's nucleotide sequence