Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0060 and Psyr_0061 overlap by 1 nucleotides Psyr_0061 and Psyr_0062 overlap by 4 nucleotides Psyr_0062 and Psyr_0063 are separated by 232 nucleotides Psyr_0063 and Psyr_0064 overlap by 4 nucleotides
Psyr_0060: Psyr_0060 - Protein of unknown function DUF513, at 59,526 to 60,701
_0060
Psyr_0061: Psyr_0061 - uroporphyrinogen-III synthase, at 60,701 to 61,474
_0061
Psyr_0062: Psyr_0062 - hydroxymethylbilane synthase, at 61,471 to 62,412
_0062
Psyr_0063: Psyr_0063 - Two-component response regulator AlgR, at 62,645 to 63,391
_0063
Psyr_0064: Psyr_0064 - Sensory transduction protein kinase AlgZ, at 63,388 to 64,470
_0064
Position (kb)
61
62
63 Strain fitness (log2 ratio)
-2
-1
0
1 at 60.627 kb on - strand at 60.650 kb on - strand at 60.650 kb on - strand at 60.673 kb on + strand at 60.677 kb on - strand at 60.677 kb on - strand at 60.682 kb on - strand at 60.682 kb on - strand at 60.702 kb on + strand at 61.494 kb on - strand at 61.494 kb on - strand at 62.448 kb on - strand at 62.448 kb on - strand at 62.448 kb on - strand at 62.448 kb on - strand at 62.505 kb on + strand at 62.506 kb on - strand at 62.506 kb on - strand at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.892 kb on + strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.893 kb on - strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.913 kb on + strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.914 kb on - strand, within Psyr_0063 at 62.968 kb on + strand, within Psyr_0063 at 62.969 kb on - strand, within Psyr_0063 at 63.097 kb on - strand, within Psyr_0063 at 63.097 kb on - strand, within Psyr_0063 at 63.097 kb on - strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.242 kb on + strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.243 kb on - strand, within Psyr_0063 at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.393 kb on + strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand at 63.394 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 60,627 - +0.2 60,650 - +0.6 60,650 - -0.4 60,673 + -0.1 60,677 - -2.2 60,677 - -0.3 60,682 - +0.9 60,682 - -1.1 60,702 + -0.6 61,494 - -0.2 61,494 - +0.4 62,448 - -0.5 62,448 - +0.8 62,448 - +0.5 62,448 - +0.0 62,505 + +0.2 62,506 - +0.4 62,506 - -1.0 62,892 + Psyr_0063 0.33 -0.4 62,892 + Psyr_0063 0.33 +0.3 62,892 + Psyr_0063 0.33 -1.1 62,892 + Psyr_0063 0.33 -0.5 62,892 + Psyr_0063 0.33 -0.3 62,892 + Psyr_0063 0.33 -0.9 62,892 + Psyr_0063 0.33 -0.5 62,892 + Psyr_0063 0.33 -0.2 62,893 - Psyr_0063 0.33 -1.3 62,893 - Psyr_0063 0.33 -0.7 62,893 - Psyr_0063 0.33 +0.2 62,893 - Psyr_0063 0.33 +1.5 62,893 - Psyr_0063 0.33 +0.2 62,893 - Psyr_0063 0.33 +0.1 62,893 - Psyr_0063 0.33 +0.4 62,913 + Psyr_0063 0.36 +0.5 62,913 + Psyr_0063 0.36 -0.7 62,913 + Psyr_0063 0.36 +0.3 62,913 + Psyr_0063 0.36 -0.7 62,914 - Psyr_0063 0.36 -0.5 62,914 - Psyr_0063 0.36 -1.9 62,914 - Psyr_0063 0.36 -1.6 62,914 - Psyr_0063 0.36 -0.1 62,914 - Psyr_0063 0.36 -0.8 62,914 - Psyr_0063 0.36 -1.1 62,968 + Psyr_0063 0.43 -1.0 62,969 - Psyr_0063 0.43 +0.1 63,097 - Psyr_0063 0.61 -0.4 63,097 - Psyr_0063 0.61 -0.2 63,097 - Psyr_0063 0.61 +0.4 63,242 + Psyr_0063 0.80 -0.6 63,242 + Psyr_0063 0.80 +0.0 63,242 + Psyr_0063 0.80 -0.6 63,242 + Psyr_0063 0.80 -0.4 63,242 + Psyr_0063 0.80 +0.0 63,242 + Psyr_0063 0.80 +0.1 63,243 - Psyr_0063 0.80 -0.3 63,243 - Psyr_0063 0.80 -0.8 63,243 - Psyr_0063 0.80 +0.6 63,243 - Psyr_0063 0.80 -2.7 63,391 + +0.4 63,391 + -0.5 63,391 + +0.3 63,391 + -0.8 63,391 + -1.5 63,391 + +0.1 63,392 - +0.5 63,392 - +0.1 63,392 - +0.3 63,392 - +0.2 63,393 + +0.4 63,393 + -0.1 63,393 + +0.1 63,393 + -0.4 63,393 + +0.1 63,393 + -0.4 63,393 + -0.2 63,393 + +0.4 63,393 + -1.2 63,393 + -0.1 63,393 + +0.0 63,393 + +0.0 63,393 + +1.6 63,393 + +0.1 63,394 - +1.5 63,394 - -0.0 63,394 - -0.2 63,394 - -0.2 63,394 - -0.3 63,394 - +0.1 63,394 - -0.1 63,394 - +0.5 63,394 - +0.7 63,394 - -0.5 63,394 - -0.2 63,394 - -1.3 63,394 - +0.6 63,394 - -0.4 63,394 - +0.4 63,394 - -0.2 63,394 - +1.4 63,394 - +1.3 63,394 - -0.6
Or see this region's nucleotide sequence