Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0008 and Psyr_0009 are separated by 140 nucleotides Psyr_0009 and Psyr_0010 are separated by 65 nucleotides Psyr_0010 and Psyr_0011 are separated by 12 nucleotides
Psyr_0008: Psyr_0008 - transcriptional regulator, AraC family, at 9,410 to 10,384
_0008
Psyr_0009: Psyr_0009 - Phospholipid/glycerol acyltransferase, at 10,525 to 11,295
_0009
Psyr_0010: Psyr_0010 - D-alpha,beta-D-heptose 1,7-bisphosphate phosphatase, at 11,361 to 11,903
_0010
Psyr_0011: Psyr_0011 - glycyl-tRNA synthetase beta chain, at 11,916 to 13,970
_0011
Position (kb)
11
12 Strain fitness (log2 ratio)
-1
0
1 at 10.366 kb on + strand at 10.382 kb on + strand at 10.399 kb on + strand at 10.399 kb on + strand at 10.400 kb on - strand at 10.414 kb on + strand at 10.455 kb on - strand at 10.456 kb on + strand at 10.506 kb on - strand at 10.506 kb on - strand at 10.656 kb on - strand, within Psyr_0009 at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 10,366 + +0.1 10,382 + -0.7 10,399 + -0.6 10,399 + -1.3 10,400 - -0.3 10,414 + +0.0 10,455 - -0.9 10,456 + -0.9 10,506 - +0.2 10,506 - -0.3 10,656 - Psyr_0009 0.17 +0.1 11,296 - -0.4 11,296 - +0.0 11,296 - +1.6 11,296 - -1.6 11,296 - +0.0 11,296 - -1.7 11,296 - -0.3 11,300 - +0.2 11,300 - +0.0 11,300 - -1.1 11,300 - -0.3 11,300 - -1.2 11,300 - -1.0 11,300 - -1.3 11,300 - -1.1 11,300 - -1.3 11,300 - -0.3 11,300 - +0.5 11,300 - +1.6
Or see this region's nucleotide sequence