Experiment: Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT_2008 and BT2009 are separated by 28 nucleotides BT2009 and BT2010 overlap by 4 nucleotides BT2010 and BT2011 are separated by 95 nucleotides BT2011 and BT2012 overlap by 38 nucleotides
BT_2008: BT_2008 - hypothetical protein (RefSeq), at 2,524,219 to 2,525,097
_2008
BT2009: BT2009 - guanylate kinase (GMP kinase) (NCBI ptt file), at 2,525,126 to 2,525,740
BT2009
BT2010: BT2010 - hypothetical protein (NCBI ptt file), at 2,525,737 to 2,525,991
BT2010
BT2011: BT2011 - putative nicotinate-nucleotide adenylyltransferase (NCBI ptt file), at 2,526,087 to 2,526,695
BT2011
BT2012: BT2012 - conserved hypothetical protein, putative ATPase (NCBI ptt file), at 2,526,658 to 2,527,947
BT2012
Position (kb)
2526
2527 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2525.831 kb on + strand, within BT2010 at 2525.835 kb on + strand, within BT2010 at 2526.041 kb on + strand at 2526.659 kb on + strand at 2526.659 kb on + strand at 2526.660 kb on - strand at 2526.668 kb on + strand at 2526.669 kb on - strand at 2526.693 kb on + strand at 2526.693 kb on + strand at 2526.694 kb on - strand at 2526.736 kb on + strand at 2526.871 kb on - strand, within BT2012 at 2526.891 kb on + strand, within BT2012 at 2526.922 kb on + strand, within BT2012 at 2526.923 kb on - strand at 2526.925 kb on + strand, within BT2012 at 2526.940 kb on - strand, within BT2012 at 2526.940 kb on - strand, within BT2012 at 2526.951 kb on + strand, within BT2012 at 2526.952 kb on - strand, within BT2012 at 2527.008 kb on + strand, within BT2012 at 2527.009 kb on - strand, within BT2012 at 2527.009 kb on - strand, within BT2012 at 2527.045 kb on - strand, within BT2012 at 2527.109 kb on + strand at 2527.109 kb on + strand, within BT2012 at 2527.114 kb on - strand, within BT2012 at 2527.192 kb on - strand, within BT2012 at 2527.296 kb on - strand, within BT2012 at 2527.296 kb on - strand, within BT2012 at 2527.350 kb on + strand, within BT2012 at 2527.388 kb on + strand, within BT2012 at 2527.388 kb on + strand, within BT2012 at 2527.463 kb on - strand, within BT2012 at 2527.522 kb on + strand, within BT2012 at 2527.566 kb on + strand, within BT2012 at 2527.567 kb on - strand, within BT2012 at 2527.634 kb on + strand, within BT2012 at 2527.687 kb on + strand, within BT2012
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM remove 2,525,831 + BT2010 0.37 +2.3 2,525,835 + BT2010 0.38 -1.9 2,526,041 + +0.0 2,526,659 + -0.8 2,526,659 + -0.4 2,526,660 - -2.3 2,526,668 + +1.8 2,526,669 - -1.0 2,526,693 + -0.8 2,526,693 + -1.0 2,526,694 - -1.0 2,526,736 + +1.6 2,526,871 - BT2012 0.17 +3.5 2,526,891 + BT2012 0.18 -0.6 2,526,922 + BT2012 0.20 +1.1 2,526,923 - -1.0 2,526,925 + BT2012 0.21 -1.0 2,526,940 - BT2012 0.22 -0.4 2,526,940 - BT2012 0.22 -3.2 2,526,951 + BT2012 0.23 +1.4 2,526,952 - BT2012 0.23 +2.6 2,527,008 + BT2012 0.27 -4.2 2,527,009 - BT2012 0.27 -0.5 2,527,009 - BT2012 0.27 -0.0 2,527,045 - BT2012 0.30 -0.5 2,527,109 + -1.0 2,527,109 + BT2012 0.35 -0.7 2,527,114 - BT2012 0.35 -0.0 2,527,192 - BT2012 0.41 -0.5 2,527,296 - BT2012 0.49 -2.3 2,527,296 - BT2012 0.49 -0.0 2,527,350 + BT2012 0.54 -0.0 2,527,388 + BT2012 0.57 -0.7 2,527,388 + BT2012 0.57 -2.8 2,527,463 - BT2012 0.62 -1.0 2,527,522 + BT2012 0.67 -0.0 2,527,566 + BT2012 0.70 -0.3 2,527,567 - BT2012 0.70 -1.6 2,527,634 + BT2012 0.76 +0.8 2,527,687 + BT2012 0.80 -0.2
Or see this region's nucleotide sequence