Experiment: Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1747 and BT1748 are separated by 124 nucleotides BT1748 and BT1749 are separated by 10 nucleotides BT1749 and BT1750 are separated by 54 nucleotides BT1750 and BT1751 overlap by 4 nucleotides
BT1747: BT1747 - pyruvate-flavodoxin oxidoreductase (NCBI ptt file), at 2,155,855 to 2,159,412
BT1747
BT1748: BT1748 - hypothetical protein (NCBI ptt file), at 2,159,537 to 2,159,725
BT1748
BT1749: BT1749 - glycine betaine-binding protein precursor (NCBI ptt file), at 2,159,736 to 2,160,569
BT1749
BT1750: BT1750 - glycine betaine/L-proline transport system permease (NCBI ptt file), at 2,160,624 to 2,161,454
BT1750
BT1751: BT1751 - Glycine betaine transport ATP-binding protein (NCBI ptt file), at 2,161,451 to 2,162,677
BT1751
Position (kb)
2159
2160
2161 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2158.774 kb on + strand, within BT1747 at 2158.881 kb on + strand, within BT1747 at 2158.882 kb on - strand, within BT1747 at 2158.963 kb on - strand, within BT1747 at 2158.965 kb on + strand, within BT1747 at 2158.976 kb on - strand, within BT1747 at 2158.979 kb on + strand, within BT1747 at 2159.089 kb on - strand at 2159.119 kb on - strand at 2159.346 kb on + strand at 2159.400 kb on + strand at 2159.410 kb on + strand at 2159.414 kb on - strand at 2159.435 kb on - strand at 2159.437 kb on + strand at 2159.487 kb on + strand at 2159.487 kb on + strand at 2159.488 kb on - strand at 2159.488 kb on - strand at 2159.488 kb on - strand at 2159.493 kb on + strand at 2159.515 kb on + strand at 2159.619 kb on + strand, within BT1748 at 2159.633 kb on - strand, within BT1748 at 2159.633 kb on - strand, within BT1748 at 2159.633 kb on - strand, within BT1748 at 2159.634 kb on + strand, within BT1748 at 2159.681 kb on + strand, within BT1748 at 2159.685 kb on - strand, within BT1748 at 2159.685 kb on - strand, within BT1748 at 2159.691 kb on - strand, within BT1748 at 2159.695 kb on + strand, within BT1748 at 2159.929 kb on - strand, within BT1749 at 2159.929 kb on - strand, within BT1749 at 2159.929 kb on - strand, within BT1749 at 2159.948 kb on + strand, within BT1749 at 2159.956 kb on + strand, within BT1749 at 2159.958 kb on + strand, within BT1749 at 2160.105 kb on - strand, within BT1749 at 2160.107 kb on - strand, within BT1749 at 2160.118 kb on + strand, within BT1749 at 2160.274 kb on + strand, within BT1749 at 2160.275 kb on - strand, within BT1749 at 2160.275 kb on - strand, within BT1749 at 2160.278 kb on + strand, within BT1749 at 2160.279 kb on - strand, within BT1749 at 2160.279 kb on - strand, within BT1749 at 2160.320 kb on - strand, within BT1749 at 2160.329 kb on + strand, within BT1749 at 2160.354 kb on - strand, within BT1749 at 2160.386 kb on + strand, within BT1749 at 2160.387 kb on - strand, within BT1749 at 2160.387 kb on - strand, within BT1749 at 2160.391 kb on - strand, within BT1749 at 2160.421 kb on - strand, within BT1749 at 2160.505 kb on - strand at 2160.553 kb on - strand at 2160.590 kb on + strand at 2160.591 kb on - strand at 2160.591 kb on - strand at 2160.591 kb on - strand at 2160.601 kb on + strand at 2160.798 kb on + strand, within BT1750 at 2160.798 kb on + strand, within BT1750 at 2160.848 kb on + strand, within BT1750 at 2160.896 kb on + strand, within BT1750 at 2160.921 kb on + strand, within BT1750 at 2160.924 kb on + strand, within BT1750 at 2161.098 kb on + strand, within BT1750 at 2161.099 kb on - strand, within BT1750 at 2161.106 kb on + strand, within BT1750 at 2161.179 kb on + strand, within BT1750 at 2161.180 kb on - strand, within BT1750 at 2161.237 kb on - strand, within BT1750 at 2161.254 kb on + strand, within BT1750 at 2161.254 kb on + strand, within BT1750 at 2161.255 kb on - strand, within BT1750 at 2161.277 kb on + strand, within BT1750 at 2161.336 kb on - strand, within BT1750 at 2161.336 kb on - strand, within BT1750 at 2161.353 kb on + strand, within BT1750 at 2161.418 kb on + strand at 2161.419 kb on - strand at 2161.454 kb on + strand at 2161.455 kb on - strand at 2161.460 kb on + strand at 2161.538 kb on + strand at 2161.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM remove 2,158,774 + BT1747 0.82 -2.1 2,158,881 + BT1747 0.85 -1.1 2,158,882 - BT1747 0.85 -1.9 2,158,963 - BT1747 0.87 -0.9 2,158,965 + BT1747 0.87 +0.8 2,158,976 - BT1747 0.88 -1.6 2,158,979 + BT1747 0.88 +0.9 2,159,089 - +0.5 2,159,119 - -3.4 2,159,346 + +0.4 2,159,400 + -0.4 2,159,410 + +1.5 2,159,414 - -4.0 2,159,435 - +3.4 2,159,437 + -3.9 2,159,487 + -1.6 2,159,487 + +0.5 2,159,488 - -1.6 2,159,488 - -2.9 2,159,488 - -2.1 2,159,493 + -1.1 2,159,515 + +1.9 2,159,619 + BT1748 0.43 -2.9 2,159,633 - BT1748 0.51 -0.6 2,159,633 - BT1748 0.51 +3.1 2,159,633 - BT1748 0.51 -0.8 2,159,634 + BT1748 0.51 -2.6 2,159,681 + BT1748 0.76 +2.3 2,159,685 - BT1748 0.78 -0.2 2,159,685 - BT1748 0.78 -0.1 2,159,691 - BT1748 0.81 -1.6 2,159,695 + BT1748 0.84 -0.9 2,159,929 - BT1749 0.23 +0.2 2,159,929 - BT1749 0.23 +0.9 2,159,929 - BT1749 0.23 +2.3 2,159,948 + BT1749 0.25 -0.6 2,159,956 + BT1749 0.26 +2.1 2,159,958 + BT1749 0.27 +1.5 2,160,105 - BT1749 0.44 -2.4 2,160,107 - BT1749 0.44 -2.9 2,160,118 + BT1749 0.46 -2.4 2,160,274 + BT1749 0.65 -1.1 2,160,275 - BT1749 0.65 -3.8 2,160,275 - BT1749 0.65 -2.9 2,160,278 + BT1749 0.65 +0.2 2,160,279 - BT1749 0.65 -2.1 2,160,279 - BT1749 0.65 -1.9 2,160,320 - BT1749 0.70 -3.1 2,160,329 + BT1749 0.71 -1.1 2,160,354 - BT1749 0.74 +0.5 2,160,386 + BT1749 0.78 -3.2 2,160,387 - BT1749 0.78 -0.1 2,160,387 - BT1749 0.78 -1.3 2,160,391 - BT1749 0.79 -2.6 2,160,421 - BT1749 0.82 -1.6 2,160,505 - -3.9 2,160,553 - +0.2 2,160,590 + +0.4 2,160,591 - -1.1 2,160,591 - -1.0 2,160,591 - -3.1 2,160,601 + +1.0 2,160,798 + BT1750 0.21 -1.0 2,160,798 + BT1750 0.21 +1.1 2,160,848 + BT1750 0.27 -1.0 2,160,896 + BT1750 0.33 +0.4 2,160,921 + BT1750 0.36 -1.4 2,160,924 + BT1750 0.36 -3.0 2,161,098 + BT1750 0.57 -0.6 2,161,099 - BT1750 0.57 -1.6 2,161,106 + BT1750 0.58 -1.6 2,161,179 + BT1750 0.67 -1.6 2,161,180 - BT1750 0.67 -0.6 2,161,237 - BT1750 0.74 +4.3 2,161,254 + BT1750 0.76 -4.1 2,161,254 + BT1750 0.76 +0.5 2,161,255 - BT1750 0.76 -0.0 2,161,277 + BT1750 0.79 -1.5 2,161,336 - BT1750 0.86 +0.8 2,161,336 - BT1750 0.86 -0.6 2,161,353 + BT1750 0.88 -3.2 2,161,418 + +0.2 2,161,419 - -0.0 2,161,454 + -0.4 2,161,455 - +1.0 2,161,460 + -3.2 2,161,538 + +3.0 2,161,538 + +0.0
Or see this region's nucleotide sequence