Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1443

Experiment: Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1442 and BT1443 are separated by 31 nucleotidesBT1443 and BT1444 overlap by 4 nucleotidesBT1444 and BT1445 are separated by 10 nucleotides BT1442: BT1442 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase (NCBI ptt file), at 1,775,436 to 1,777,850 BT1442 BT1443: BT1443 - 8-amino-7-oxononanoate synthase (NCBI ptt file), at 1,777,882 to 1,779,036 BT1443 BT1444: BT1444 - conserved hypothetical protein (NCBI ptt file), at 1,779,033 to 1,779,692 BT1444 BT1445: BT1445 - putative biotin synthesis protein bioC (NCBI ptt file), at 1,779,703 to 1,780,488 BT1445 Position (kb) 1777 1778 1779 1780Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1776.955 kb on - strand, within BT1442at 1777.242 kb on + strand, within BT1442at 1777.243 kb on - strand, within BT1442at 1777.292 kb on + strand, within BT1442at 1777.451 kb on - strand, within BT1442at 1777.457 kb on + strand, within BT1442at 1777.457 kb on + strand, within BT1442at 1777.458 kb on - strand, within BT1442at 1777.461 kb on - strand, within BT1442at 1777.488 kb on - strand, within BT1442at 1777.500 kb on - strand, within BT1442at 1777.661 kb on - strandat 1777.669 kb on + strandat 1777.669 kb on + strandat 1777.707 kb on + strandat 1777.736 kb on + strandat 1777.805 kb on - strandat 1777.829 kb on + strandat 1777.849 kb on - strandat 1777.853 kb on + strandat 1777.853 kb on + strandat 1777.854 kb on - strandat 1777.876 kb on + strandat 1777.937 kb on - strandat 1777.962 kb on + strandat 1778.048 kb on + strand, within BT1443at 1778.049 kb on - strand, within BT1443at 1778.166 kb on + strand, within BT1443at 1778.207 kb on - strand, within BT1443at 1778.225 kb on + strand, within BT1443at 1778.250 kb on + strand, within BT1443at 1778.277 kb on - strand, within BT1443at 1778.306 kb on + strand, within BT1443at 1778.386 kb on + strand, within BT1443at 1778.460 kb on - strand, within BT1443at 1778.541 kb on - strand, within BT1443at 1778.626 kb on + strand, within BT1443at 1778.627 kb on - strand, within BT1443at 1778.627 kb on - strand, within BT1443at 1778.683 kb on - strand, within BT1443at 1778.800 kb on + strand, within BT1443at 1778.821 kb on + strand, within BT1443at 1778.824 kb on - strand, within BT1443at 1778.824 kb on - strand, within BT1443at 1778.824 kb on - strand, within BT1443at 1778.917 kb on + strand, within BT1443at 1778.918 kb on - strand, within BT1443at 1779.017 kb on + strandat 1779.122 kb on + strand, within BT1444at 1779.190 kb on + strand, within BT1444at 1779.392 kb on - strand, within BT1444at 1779.451 kb on + strand, within BT1444at 1779.686 kb on + strandat 1779.708 kb on + strandat 1779.708 kb on + strandat 1779.923 kb on + strand, within BT1445at 1779.941 kb on - strand, within BT1445at 1779.956 kb on + strand, within BT1445at 1780.024 kb on - strand, within BT1445at 1780.032 kb on + strand, within BT1445at 1780.033 kb on - strand, within BT1445at 1780.033 kb on - strand, within BT1445

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM
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1,776,955 - BT1442 0.63 -2.8
1,777,242 + BT1442 0.75 -1.6
1,777,243 - BT1442 0.75 -1.6
1,777,292 + BT1442 0.77 -2.3
1,777,451 - BT1442 0.83 -3.3
1,777,457 + BT1442 0.84 -2.8
1,777,457 + BT1442 0.84 -1.6
1,777,458 - BT1442 0.84 -4.0
1,777,461 - BT1442 0.84 +0.0
1,777,488 - BT1442 0.85 -1.0
1,777,500 - BT1442 0.85 -1.0
1,777,661 - -3.9
1,777,669 + -1.0
1,777,669 + -2.6
1,777,707 + -1.0
1,777,736 + -2.0
1,777,805 - +0.0
1,777,829 + -1.6
1,777,849 - -2.6
1,777,853 + +1.3
1,777,853 + +0.8
1,777,854 - -2.8
1,777,876 + +0.0
1,777,937 - -1.6
1,777,962 + -2.6
1,778,048 + BT1443 0.14 -5.2
1,778,049 - BT1443 0.14 -2.3
1,778,166 + BT1443 0.25 -2.3
1,778,207 - BT1443 0.28 -1.6
1,778,225 + BT1443 0.30 -3.0
1,778,250 + BT1443 0.32 -1.0
1,778,277 - BT1443 0.34 -3.0
1,778,306 + BT1443 0.37 -2.7
1,778,386 + BT1443 0.44 -1.0
1,778,460 - BT1443 0.50 -2.8
1,778,541 - BT1443 0.57 -2.0
1,778,626 + BT1443 0.64 -0.4
1,778,627 - BT1443 0.65 +0.0
1,778,627 - BT1443 0.65 -1.6
1,778,683 - BT1443 0.69 -5.1
1,778,800 + BT1443 0.79 +0.0
1,778,821 + BT1443 0.81 -2.0
1,778,824 - BT1443 0.82 -1.6
1,778,824 - BT1443 0.82 -1.6
1,778,824 - BT1443 0.82 +0.0
1,778,917 + BT1443 0.90 -3.2
1,778,918 - BT1443 0.90 -1.6
1,779,017 + -1.6
1,779,122 + BT1444 0.13 -3.4
1,779,190 + BT1444 0.24 -0.6
1,779,392 - BT1444 0.54 -2.3
1,779,451 + BT1444 0.63 -2.3
1,779,686 + -1.0
1,779,708 + +0.0
1,779,708 + -2.8
1,779,923 + BT1445 0.28 -3.2
1,779,941 - BT1445 0.30 -2.6
1,779,956 + BT1445 0.32 -2.6
1,780,024 - BT1445 0.41 -1.0
1,780,032 + BT1445 0.42 -1.0
1,780,033 - BT1445 0.42 -1.0
1,780,033 - BT1445 0.42 -1.6

Or see this region's nucleotide sequence