Experiment: Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1442 and BT1443 are separated by 31 nucleotides BT1443 and BT1444 overlap by 4 nucleotides BT1444 and BT1445 are separated by 10 nucleotides
BT1442: BT1442 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase (NCBI ptt file), at 1,775,436 to 1,777,850
BT1442
BT1443: BT1443 - 8-amino-7-oxononanoate synthase (NCBI ptt file), at 1,777,882 to 1,779,036
BT1443
BT1444: BT1444 - conserved hypothetical protein (NCBI ptt file), at 1,779,033 to 1,779,692
BT1444
BT1445: BT1445 - putative biotin synthesis protein bioC (NCBI ptt file), at 1,779,703 to 1,780,488
BT1445
Position (kb)
1777
1778
1779
1780 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1776.955 kb on - strand, within BT1442 at 1777.242 kb on + strand, within BT1442 at 1777.243 kb on - strand, within BT1442 at 1777.292 kb on + strand, within BT1442 at 1777.451 kb on - strand, within BT1442 at 1777.457 kb on + strand, within BT1442 at 1777.457 kb on + strand, within BT1442 at 1777.458 kb on - strand, within BT1442 at 1777.461 kb on - strand, within BT1442 at 1777.488 kb on - strand, within BT1442 at 1777.500 kb on - strand, within BT1442 at 1777.661 kb on - strand at 1777.669 kb on + strand at 1777.669 kb on + strand at 1777.707 kb on + strand at 1777.736 kb on + strand at 1777.805 kb on - strand at 1777.829 kb on + strand at 1777.849 kb on - strand at 1777.853 kb on + strand at 1777.853 kb on + strand at 1777.854 kb on - strand at 1777.876 kb on + strand at 1777.937 kb on - strand at 1777.962 kb on + strand at 1778.048 kb on + strand, within BT1443 at 1778.049 kb on - strand, within BT1443 at 1778.166 kb on + strand, within BT1443 at 1778.207 kb on - strand, within BT1443 at 1778.225 kb on + strand, within BT1443 at 1778.250 kb on + strand, within BT1443 at 1778.277 kb on - strand, within BT1443 at 1778.306 kb on + strand, within BT1443 at 1778.386 kb on + strand, within BT1443 at 1778.460 kb on - strand, within BT1443 at 1778.541 kb on - strand, within BT1443 at 1778.626 kb on + strand, within BT1443 at 1778.627 kb on - strand, within BT1443 at 1778.627 kb on - strand, within BT1443 at 1778.683 kb on - strand, within BT1443 at 1778.800 kb on + strand, within BT1443 at 1778.821 kb on + strand, within BT1443 at 1778.824 kb on - strand, within BT1443 at 1778.824 kb on - strand, within BT1443 at 1778.824 kb on - strand, within BT1443 at 1778.917 kb on + strand, within BT1443 at 1778.918 kb on - strand, within BT1443 at 1779.017 kb on + strand at 1779.122 kb on + strand, within BT1444 at 1779.190 kb on + strand, within BT1444 at 1779.392 kb on - strand, within BT1444 at 1779.451 kb on + strand, within BT1444 at 1779.686 kb on + strand at 1779.708 kb on + strand at 1779.708 kb on + strand at 1779.923 kb on + strand, within BT1445 at 1779.941 kb on - strand, within BT1445 at 1779.956 kb on + strand, within BT1445 at 1780.024 kb on - strand, within BT1445 at 1780.032 kb on + strand, within BT1445 at 1780.033 kb on - strand, within BT1445 at 1780.033 kb on - strand, within BT1445
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM remove 1,776,955 - BT1442 0.63 -2.8 1,777,242 + BT1442 0.75 -1.6 1,777,243 - BT1442 0.75 -1.6 1,777,292 + BT1442 0.77 -2.3 1,777,451 - BT1442 0.83 -3.3 1,777,457 + BT1442 0.84 -2.8 1,777,457 + BT1442 0.84 -1.6 1,777,458 - BT1442 0.84 -4.0 1,777,461 - BT1442 0.84 +0.0 1,777,488 - BT1442 0.85 -1.0 1,777,500 - BT1442 0.85 -1.0 1,777,661 - -3.9 1,777,669 + -1.0 1,777,669 + -2.6 1,777,707 + -1.0 1,777,736 + -2.0 1,777,805 - +0.0 1,777,829 + -1.6 1,777,849 - -2.6 1,777,853 + +1.3 1,777,853 + +0.8 1,777,854 - -2.8 1,777,876 + +0.0 1,777,937 - -1.6 1,777,962 + -2.6 1,778,048 + BT1443 0.14 -5.2 1,778,049 - BT1443 0.14 -2.3 1,778,166 + BT1443 0.25 -2.3 1,778,207 - BT1443 0.28 -1.6 1,778,225 + BT1443 0.30 -3.0 1,778,250 + BT1443 0.32 -1.0 1,778,277 - BT1443 0.34 -3.0 1,778,306 + BT1443 0.37 -2.7 1,778,386 + BT1443 0.44 -1.0 1,778,460 - BT1443 0.50 -2.8 1,778,541 - BT1443 0.57 -2.0 1,778,626 + BT1443 0.64 -0.4 1,778,627 - BT1443 0.65 +0.0 1,778,627 - BT1443 0.65 -1.6 1,778,683 - BT1443 0.69 -5.1 1,778,800 + BT1443 0.79 +0.0 1,778,821 + BT1443 0.81 -2.0 1,778,824 - BT1443 0.82 -1.6 1,778,824 - BT1443 0.82 -1.6 1,778,824 - BT1443 0.82 +0.0 1,778,917 + BT1443 0.90 -3.2 1,778,918 - BT1443 0.90 -1.6 1,779,017 + -1.6 1,779,122 + BT1444 0.13 -3.4 1,779,190 + BT1444 0.24 -0.6 1,779,392 - BT1444 0.54 -2.3 1,779,451 + BT1444 0.63 -2.3 1,779,686 + -1.0 1,779,708 + +0.0 1,779,708 + -2.8 1,779,923 + BT1445 0.28 -3.2 1,779,941 - BT1445 0.30 -2.6 1,779,956 + BT1445 0.32 -2.6 1,780,024 - BT1445 0.41 -1.0 1,780,032 + BT1445 0.42 -1.0 1,780,033 - BT1445 0.42 -1.0 1,780,033 - BT1445 0.42 -1.6
Or see this region's nucleotide sequence