Experiment: Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1250 and BT1251 overlap by 20 nucleotides BT1251 and BT1252 are separated by 248 nucleotides BT1252 and BT1253 are separated by 0 nucleotides BT1253 and BT1254 are separated by 24 nucleotides
BT1250: BT1250 - conserved hypothetical protein, putative outer membrane efflux protein (NCBI ptt file), at 1,556,809 to 1,558,128
BT1250
BT1251: BT1251 - transcriptional regulator, MarR family (NCBI ptt file), at 1,558,109 to 1,558,552
BT1251
BT1252: BT1252 - hypothetical protein (NCBI ptt file), at 1,558,801 to 1,559,352
BT1252
BT1253: BT1253 - conserved hypothetical protein (NCBI ptt file), at 1,559,353 to 1,559,649
BT1253
BT1254: BT1254 - inner membrane protein (NCBI ptt file), at 1,559,674 to 1,561,029
BT1254
Position (kb)
1558
1559
1560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1557.831 kb on + strand, within BT1250 at 1558.106 kb on - strand at 1558.113 kb on - strand at 1558.131 kb on + strand at 1558.132 kb on - strand at 1558.132 kb on - strand at 1558.200 kb on + strand, within BT1251 at 1558.591 kb on - strand at 1558.591 kb on - strand at 1558.772 kb on + strand at 1558.822 kb on - strand at 1558.845 kb on + strand at 1558.879 kb on + strand, within BT1252 at 1558.879 kb on + strand, within BT1252 at 1558.880 kb on - strand, within BT1252 at 1558.917 kb on - strand, within BT1252 at 1558.958 kb on + strand, within BT1252 at 1558.958 kb on + strand, within BT1252 at 1558.988 kb on + strand, within BT1252 at 1559.094 kb on - strand, within BT1252 at 1559.120 kb on - strand, within BT1252 at 1559.123 kb on - strand, within BT1252 at 1559.223 kb on + strand, within BT1252 at 1559.413 kb on - strand, within BT1253 at 1559.668 kb on + strand at 1559.961 kb on - strand, within BT1254 at 1560.107 kb on + strand, within BT1254 at 1560.175 kb on - strand, within BT1254
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM remove 1,557,831 + BT1250 0.77 -1.8 1,558,106 - +2.0 1,558,113 - -2.2 1,558,131 + +0.6 1,558,132 - -2.5 1,558,132 - -0.2 1,558,200 + BT1251 0.20 -1.2 1,558,591 - -0.2 1,558,591 - +1.5 1,558,772 + +1.6 1,558,822 - -1.2 1,558,845 + +1.9 1,558,879 + BT1252 0.14 -2.2 1,558,879 + BT1252 0.14 -3.8 1,558,880 - BT1252 0.14 -0.5 1,558,917 - BT1252 0.21 -2.2 1,558,958 + BT1252 0.28 -1.8 1,558,958 + BT1252 0.28 -4.3 1,558,988 + BT1252 0.34 -1.4 1,559,094 - BT1252 0.53 -0.2 1,559,120 - BT1252 0.58 +1.0 1,559,123 - BT1252 0.58 +1.4 1,559,223 + BT1252 0.76 -2.5 1,559,413 - BT1253 0.20 -3.0 1,559,668 + +0.4 1,559,961 - BT1254 0.21 -0.3 1,560,107 + BT1254 0.32 +1.4 1,560,175 - BT1254 0.37 -0.2
Or see this region's nucleotide sequence