Experiment: Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0233 and BT0234 are separated by 128 nucleotides BT0234 and BT0235 are separated by 457 nucleotides
BT0233: BT0233 - integrase (NCBI ptt file), at 253,724 to 254,845
BT0233
BT0234: BT0234 - putative transposase (NCBI ptt file), at 254,974 to 255,924
BT0234
BT0235: BT0235 - conserved hypothetical protein (NCBI ptt file), at 256,382 to 257,509
BT0235
Position (kb) 254
255
256 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 253.990 kb on + strand, within BT0233 at 253.991 kb on - strand, within BT0233 at 254.033 kb on - strand, within BT0233 at 254.080 kb on - strand, within BT0233 at 254.101 kb on - strand, within BT0233 at 254.109 kb on - strand, within BT0233 at 254.156 kb on + strand, within BT0233 at 254.371 kb on + strand, within BT0233 at 254.389 kb on + strand, within BT0233 at 254.418 kb on + strand, within BT0233 at 254.465 kb on - strand, within BT0233 at 254.465 kb on - strand, within BT0233 at 254.497 kb on + strand, within BT0233 at 254.499 kb on + strand, within BT0233 at 254.500 kb on - strand, within BT0233 at 254.698 kb on - strand, within BT0233 at 254.704 kb on - strand, within BT0233 at 254.704 kb on - strand, within BT0233 at 254.766 kb on + strand at 254.778 kb on + strand at 254.778 kb on + strand at 254.822 kb on + strand at 254.822 kb on + strand at 254.907 kb on + strand at 254.985 kb on + strand at 255.052 kb on + strand at 255.058 kb on - strand at 255.081 kb on + strand, within BT0234 at 255.112 kb on + strand, within BT0234 at 255.113 kb on - strand, within BT0234 at 255.113 kb on - strand, within BT0234 at 255.141 kb on - strand, within BT0234 at 255.141 kb on - strand, within BT0234 at 255.141 kb on - strand, within BT0234 at 255.157 kb on - strand, within BT0234 at 255.201 kb on - strand, within BT0234 at 255.204 kb on + strand, within BT0234 at 255.204 kb on + strand, within BT0234 at 255.234 kb on + strand, within BT0234 at 255.242 kb on + strand, within BT0234 at 255.277 kb on - strand, within BT0234 at 255.277 kb on - strand, within BT0234 at 255.290 kb on - strand, within BT0234 at 255.357 kb on + strand, within BT0234 at 255.428 kb on + strand, within BT0234 at 255.623 kb on - strand, within BT0234 at 255.703 kb on - strand, within BT0234 at 255.704 kb on + strand, within BT0234 at 255.705 kb on - strand, within BT0234 at 255.725 kb on - strand, within BT0234 at 255.747 kb on + strand, within BT0234 at 255.753 kb on - strand, within BT0234 at 255.925 kb on - strand at 255.940 kb on + strand at 255.940 kb on + strand at 256.241 kb on - strand at 256.288 kb on - strand at 256.307 kb on + strand at 256.436 kb on - strand at 256.539 kb on + strand, within BT0235 at 256.651 kb on - strand, within BT0235 at 256.660 kb on + strand, within BT0235 at 256.685 kb on - strand, within BT0235 at 256.718 kb on + strand, within BT0235 at 256.743 kb on - strand, within BT0235 at 256.770 kb on - strand, within BT0235 at 256.770 kb on - strand, within BT0235 at 256.801 kb on - strand, within BT0235 at 256.880 kb on - strand, within BT0235
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM remove 253,990 + BT0233 0.24 +2.3 253,991 - BT0233 0.24 -2.2 254,033 - BT0233 0.28 -0.2 254,080 - BT0233 0.32 -0.2 254,101 - BT0233 0.34 -1.5 254,109 - BT0233 0.34 -3.0 254,156 + BT0233 0.39 -1.7 254,371 + BT0233 0.58 -1.2 254,389 + BT0233 0.59 -0.5 254,418 + BT0233 0.62 -0.2 254,465 - BT0233 0.66 -2.2 254,465 - BT0233 0.66 -2.2 254,497 + BT0233 0.69 -1.2 254,499 + BT0233 0.69 -0.2 254,500 - BT0233 0.69 -0.4 254,698 - BT0233 0.87 -1.2 254,704 - BT0233 0.87 -1.8 254,704 - BT0233 0.87 -1.7 254,766 + -0.2 254,778 + -2.5 254,778 + +0.8 254,822 + +2.3 254,822 + +0.6 254,907 + -4.0 254,985 + -1.2 255,052 + -1.3 255,058 - -2.5 255,081 + BT0234 0.11 -1.7 255,112 + BT0234 0.15 +0.9 255,113 - BT0234 0.15 -1.6 255,113 - BT0234 0.15 -2.5 255,141 - BT0234 0.18 -0.2 255,141 - BT0234 0.18 -3.3 255,141 - BT0234 0.18 -2.5 255,157 - BT0234 0.19 -2.6 255,201 - BT0234 0.24 -1.2 255,204 + BT0234 0.24 -1.7 255,204 + BT0234 0.24 +2.3 255,234 + BT0234 0.27 -2.7 255,242 + BT0234 0.28 -1.3 255,277 - BT0234 0.32 -2.2 255,277 - BT0234 0.32 -3.6 255,290 - BT0234 0.33 +0.3 255,357 + BT0234 0.40 -1.7 255,428 + BT0234 0.48 -2.2 255,623 - BT0234 0.68 -1.0 255,703 - BT0234 0.77 -0.2 255,704 + BT0234 0.77 -1.8 255,705 - BT0234 0.77 -1.2 255,725 - BT0234 0.79 +1.4 255,747 + BT0234 0.81 -1.6 255,753 - BT0234 0.82 -1.2 255,925 - +1.1 255,940 + +2.2 255,940 + -1.2 256,241 - -2.5 256,288 - -1.2 256,307 + -4.4 256,436 - -1.8 256,539 + BT0235 0.14 +2.8 256,651 - BT0235 0.24 -1.2 256,660 + BT0235 0.25 -2.1 256,685 - BT0235 0.27 -1.8 256,718 + BT0235 0.30 -2.5 256,743 - BT0235 0.32 -1.2 256,770 - BT0235 0.34 -0.9 256,770 - BT0235 0.34 +0.2 256,801 - BT0235 0.37 -2.2 256,880 - BT0235 0.44 -2.0
Or see this region's nucleotide sequence