Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_77

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
80,281	-		Pf1N1B4_76	0.70	-0.7
80,281	-		Pf1N1B4_76	0.70	+0.7
80,344	-		Pf1N1B4_76	0.74	-1.8
80,374	-		Pf1N1B4_76	0.77	+1.4
80,449	-		Pf1N1B4_76	0.82	-0.7
80,503	-		Pf1N1B4_76	0.86	-2.1
80,659	-				-0.4
80,705	+				-2.5
80,752	+				-0.3
80,752	+				-0.5
80,752	+				-0.0
80,752	+				-1.7
80,753	-				-0.3
80,753	-				+0.4
80,753	-				+0.3
80,753	-				+0.0
80,824	+				+3.0
80,824	+				+1.8
80,824	+				-0.6
80,825	-				+1.2
80,825	-				+1.0
80,877	-				-1.4
80,877	-				-0.8
80,911	+				+1.0
80,927	-				-0.8
81,030	+				-0.3
81,030	+				-1.0
81,031	-				-1.4
81,071	+		Pf1N1B4_77	0.22	-2.4
81,071	+		Pf1N1B4_77	0.22	-0.8
81,071	+		Pf1N1B4_77	0.22	-1.7
81,137	+		Pf1N1B4_77	0.43	-0.8
81,162	+		Pf1N1B4_77	0.51	-0.8
81,220	+		Pf1N1B4_77	0.69	-0.5
81,289	+				-1.0
81,290	-				+0.2
81,290	-				-1.7
81,315	+				-0.2
81,316	-				+0.2
81,334	+				+0.2
81,335	-				-2.3
81,335	-				-1.5
81,403	-				+1.8
81,403	-				-1.3
81,426	+				-1.0
81,778	-		Pf1N1B4_78	0.59	-1.4
81,778	-		Pf1N1B4_78	0.59	-2.9
81,778	-		Pf1N1B4_78	0.59	+0.9
81,778	-		Pf1N1B4_78	0.59	-1.4
81,967	+				+0.1
81,968	-				-1.6
82,022	+				-1.0
82,064	+				-0.2
82,064	+				+0.4
82,064	+				+1.4
82,065	-				+0.4
82,065	-				-1.0
82,065	-				+0.8
82,065	-				-1.5
82,065	-				-0.1
82,070	+				-0.8
82,071	-				+0.6
82,071	-				+0.8
82,071	-				+0.5
82,090	+				-0.2
82,090	+				+0.2
82,091	-				-1.7
82,091	-				-0.9
82,091	-				+1.0
82,124	+				+0.5
82,125	-				+1.2