Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5749 and Pf1N1B4_5750 are separated by 240 nucleotides Pf1N1B4_5750 and Pf1N1B4_5751 are separated by 78 nucleotides Pf1N1B4_5751 and Pf1N1B4_5752 are separated by 154 nucleotides Pf1N1B4_5752 and Pf1N1B4_5753 are separated by 75 nucleotides
Pf1N1B4_5749 - Molybdenum cofactor biosynthesis protein MoaB, at 189,990 to 190,544
_5749
Pf1N1B4_5750 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 190,785 to 191,432
_5750
Pf1N1B4_5751 - Functional role page for Anaerobic nitric oxide reductase transcription regulator NorR, at 191,511 to 193,040
_5751
Pf1N1B4_5752 - Nitric oxide-dependent regulator DnrN or NorA, at 193,195 to 193,869
_5752
Pf1N1B4_5753 - putative membrane protein, at 193,945 to 194,382
_5753
Position (kb) 191
192
193
194 Strain fitness (log2 ratio) -2
-1
0
1 at 190.624 kb on - strand at 190.640 kb on - strand at 190.773 kb on + strand at 190.813 kb on - strand at 190.813 kb on - strand at 191.057 kb on - strand, within Pf1N1B4_5750 at 191.188 kb on + strand, within Pf1N1B4_5750 at 191.189 kb on - strand, within Pf1N1B4_5750 at 191.399 kb on - strand at 191.681 kb on + strand, within Pf1N1B4_5751 at 191.852 kb on + strand, within Pf1N1B4_5751 at 191.854 kb on + strand at 191.854 kb on + strand, within Pf1N1B4_5751 at 191.855 kb on - strand, within Pf1N1B4_5751 at 192.014 kb on - strand, within Pf1N1B4_5751 at 192.320 kb on + strand, within Pf1N1B4_5751 at 192.321 kb on - strand at 192.327 kb on + strand, within Pf1N1B4_5751 at 192.438 kb on + strand at 192.694 kb on + strand, within Pf1N1B4_5751 at 192.694 kb on + strand, within Pf1N1B4_5751 at 192.694 kb on + strand, within Pf1N1B4_5751 at 192.695 kb on - strand, within Pf1N1B4_5751 at 192.763 kb on + strand, within Pf1N1B4_5751 at 192.952 kb on + strand at 192.953 kb on - strand at 193.044 kb on - strand at 193.044 kb on - strand at 193.047 kb on - strand at 193.047 kb on - strand at 193.058 kb on - strand at 193.755 kb on + strand, within Pf1N1B4_5752 at 193.942 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 190,624 - -0.9 190,640 - -1.3 190,773 + +0.1 190,813 - +1.2 190,813 - -1.6 191,057 - Pf1N1B4_5750 0.42 +1.2 191,188 + Pf1N1B4_5750 0.62 -0.6 191,189 - Pf1N1B4_5750 0.62 -2.4 191,399 - -0.1 191,681 + Pf1N1B4_5751 0.11 -2.0 191,852 + Pf1N1B4_5751 0.22 -1.0 191,854 + -1.0 191,854 + Pf1N1B4_5751 0.22 -1.7 191,855 - Pf1N1B4_5751 0.22 -0.7 192,014 - Pf1N1B4_5751 0.33 -1.6 192,320 + Pf1N1B4_5751 0.53 -1.4 192,321 - -1.0 192,327 + Pf1N1B4_5751 0.53 -1.0 192,438 + -0.0 192,694 + Pf1N1B4_5751 0.77 -0.8 192,694 + Pf1N1B4_5751 0.77 +0.8 192,694 + Pf1N1B4_5751 0.77 +0.5 192,695 - Pf1N1B4_5751 0.77 -1.1 192,763 + Pf1N1B4_5751 0.82 -1.7 192,952 + -1.0 192,953 - +0.1 193,044 - +0.4 193,044 - +1.1 193,047 - -0.2 193,047 - -0.5 193,058 - -0.7 193,755 + Pf1N1B4_5752 0.83 +1.2 193,942 + -1.8
Or see this region's nucleotide sequence