Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_551 and Pf1N1B4_552 are separated by 123 nucleotides Pf1N1B4_552 and Pf1N1B4_553 are separated by 101 nucleotides Pf1N1B4_553 and Pf1N1B4_554 are separated by 66 nucleotides Pf1N1B4_554 and Pf1N1B4_555 are separated by 137 nucleotides
Pf1N1B4_551 - Lipid A export ATP-binding/permease protein MsbA, at 647,752 to 649,584
_551
Pf1N1B4_552 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 649,708 to 650,196
_552
Pf1N1B4_553 - Bacterial transcription activator, effector binding protein, at 650,298 to 650,786
_553
Pf1N1B4_554 - Putative threonine efflux protein, at 650,853 to 651,515
_554
Pf1N1B4_555 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 651,653 to 652,870
_555
Position (kb) 650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 649.534 kb on + strand at 649.535 kb on - strand at 649.772 kb on + strand, within Pf1N1B4_552 at 649.773 kb on - strand, within Pf1N1B4_552 at 649.773 kb on - strand, within Pf1N1B4_552 at 649.773 kb on - strand, within Pf1N1B4_552 at 649.793 kb on + strand, within Pf1N1B4_552 at 649.793 kb on + strand, within Pf1N1B4_552 at 649.793 kb on + strand, within Pf1N1B4_552 at 649.794 kb on - strand, within Pf1N1B4_552 at 649.803 kb on + strand, within Pf1N1B4_552 at 649.804 kb on - strand, within Pf1N1B4_552 at 649.905 kb on - strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.147 kb on + strand, within Pf1N1B4_552 at 650.148 kb on - strand at 650.148 kb on - strand at 650.148 kb on - strand at 650.233 kb on + strand at 650.233 kb on + strand at 650.233 kb on + strand at 650.233 kb on + strand at 650.299 kb on + strand at 650.300 kb on - strand at 650.300 kb on - strand at 650.300 kb on - strand at 650.307 kb on + strand at 650.365 kb on + strand, within Pf1N1B4_553 at 650.366 kb on - strand, within Pf1N1B4_553 at 650.431 kb on + strand, within Pf1N1B4_553 at 650.431 kb on + strand, within Pf1N1B4_553 at 650.431 kb on + strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.432 kb on - strand, within Pf1N1B4_553 at 650.486 kb on - strand, within Pf1N1B4_553 at 650.486 kb on - strand, within Pf1N1B4_553 at 650.488 kb on + strand, within Pf1N1B4_553 at 650.489 kb on - strand, within Pf1N1B4_553 at 650.499 kb on + strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.500 kb on - strand, within Pf1N1B4_553 at 650.515 kb on + strand, within Pf1N1B4_553 at 650.563 kb on + strand, within Pf1N1B4_553 at 650.564 kb on - strand, within Pf1N1B4_553 at 650.592 kb on - strand, within Pf1N1B4_553 at 650.605 kb on + strand, within Pf1N1B4_553 at 650.605 kb on + strand, within Pf1N1B4_553 at 650.606 kb on - strand, within Pf1N1B4_553 at 650.827 kb on - strand at 650.827 kb on - strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.828 kb on + strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.829 kb on - strand at 650.855 kb on - strand at 650.855 kb on - strand at 650.855 kb on - strand at 650.900 kb on + strand at 650.900 kb on + strand at 650.901 kb on - strand at 650.901 kb on - strand at 650.933 kb on - strand, within Pf1N1B4_554 at 650.966 kb on - strand, within Pf1N1B4_554 at 651.254 kb on - strand, within Pf1N1B4_554 at 651.254 kb on - strand, within Pf1N1B4_554 at 651.275 kb on - strand, within Pf1N1B4_554 at 651.275 kb on - strand, within Pf1N1B4_554 at 651.275 kb on - strand, within Pf1N1B4_554 at 651.337 kb on - strand, within Pf1N1B4_554 at 651.337 kb on - strand, within Pf1N1B4_554 at 651.418 kb on + strand, within Pf1N1B4_554 at 651.419 kb on - strand, within Pf1N1B4_554 at 651.507 kb on - strand at 651.543 kb on + strand at 651.544 kb on - strand at 651.544 kb on - strand at 651.544 kb on - strand at 651.547 kb on + strand at 651.547 kb on + strand at 651.547 kb on + strand at 651.548 kb on - strand at 651.548 kb on - strand at 651.548 kb on - strand at 651.578 kb on + strand at 651.578 kb on + strand at 651.579 kb on - strand at 651.634 kb on + strand at 651.634 kb on + strand at 651.634 kb on + strand at 651.634 kb on + strand at 651.634 kb on + strand at 651.635 kb on - strand at 651.635 kb on - strand at 651.635 kb on - strand at 651.635 kb on - strand at 651.690 kb on + strand at 651.691 kb on - strand at 651.691 kb on - strand at 651.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 649,534 + -1.5 649,535 - -1.4 649,772 + Pf1N1B4_552 0.13 +0.5 649,773 - Pf1N1B4_552 0.13 +0.1 649,773 - Pf1N1B4_552 0.13 +1.6 649,773 - Pf1N1B4_552 0.13 +2.2 649,793 + Pf1N1B4_552 0.17 -1.7 649,793 + Pf1N1B4_552 0.17 +1.2 649,793 + Pf1N1B4_552 0.17 +0.8 649,794 - Pf1N1B4_552 0.18 +0.2 649,803 + Pf1N1B4_552 0.19 -0.7 649,804 - Pf1N1B4_552 0.20 -3.1 649,905 - Pf1N1B4_552 0.40 +0.8 650,147 + Pf1N1B4_552 0.90 -0.5 650,147 + Pf1N1B4_552 0.90 +1.8 650,147 + Pf1N1B4_552 0.90 -0.8 650,147 + Pf1N1B4_552 0.90 +1.2 650,148 - -0.5 650,148 - -1.4 650,148 - -0.7 650,233 + +0.5 650,233 + -0.7 650,233 + -0.5 650,233 + +0.9 650,299 + +2.0 650,300 - -0.8 650,300 - -1.6 650,300 - -0.5 650,307 + -2.2 650,365 + Pf1N1B4_553 0.14 -1.2 650,366 - Pf1N1B4_553 0.14 +1.2 650,431 + Pf1N1B4_553 0.27 +0.4 650,431 + Pf1N1B4_553 0.27 -1.3 650,431 + Pf1N1B4_553 0.27 -1.1 650,432 - Pf1N1B4_553 0.27 -1.0 650,432 - Pf1N1B4_553 0.27 +0.4 650,432 - Pf1N1B4_553 0.27 +3.6 650,432 - Pf1N1B4_553 0.27 -2.0 650,432 - Pf1N1B4_553 0.27 -2.7 650,432 - Pf1N1B4_553 0.27 +0.8 650,432 - Pf1N1B4_553 0.27 +3.3 650,486 - Pf1N1B4_553 0.38 +2.1 650,486 - Pf1N1B4_553 0.38 +0.2 650,488 + Pf1N1B4_553 0.39 -2.7 650,489 - Pf1N1B4_553 0.39 -1.7 650,499 + Pf1N1B4_553 0.41 +0.2 650,500 - Pf1N1B4_553 0.41 -1.6 650,500 - Pf1N1B4_553 0.41 -1.0 650,500 - Pf1N1B4_553 0.41 -1.9 650,500 - Pf1N1B4_553 0.41 -1.0 650,515 + Pf1N1B4_553 0.44 +0.1 650,563 + Pf1N1B4_553 0.54 +2.1 650,564 - Pf1N1B4_553 0.54 -1.8 650,592 - Pf1N1B4_553 0.60 +0.5 650,605 + Pf1N1B4_553 0.63 -1.2 650,605 + Pf1N1B4_553 0.63 +1.4 650,606 - Pf1N1B4_553 0.63 -0.8 650,827 - -2.1 650,827 - -1.8 650,828 + +0.6 650,828 + -0.2 650,828 + +1.7 650,828 + -0.5 650,828 + +0.6 650,828 + -0.8 650,828 + -2.5 650,829 - +1.1 650,829 - -2.3 650,829 - -0.8 650,829 - -2.0 650,829 - -1.0 650,829 - -0.8 650,829 - +0.6 650,855 - +1.2 650,855 - +0.3 650,855 - -0.8 650,900 + -0.8 650,900 + +1.1 650,901 - -1.2 650,901 - -2.8 650,933 - Pf1N1B4_554 0.12 -1.6 650,966 - Pf1N1B4_554 0.17 -1.2 651,254 - Pf1N1B4_554 0.60 +1.2 651,254 - Pf1N1B4_554 0.60 -0.3 651,275 - Pf1N1B4_554 0.64 -0.6 651,275 - Pf1N1B4_554 0.64 +1.2 651,275 - Pf1N1B4_554 0.64 -0.5 651,337 - Pf1N1B4_554 0.73 -2.3 651,337 - Pf1N1B4_554 0.73 -0.2 651,418 + Pf1N1B4_554 0.85 +0.6 651,419 - Pf1N1B4_554 0.85 -1.2 651,507 - +0.7 651,543 + +0.2 651,544 - -0.8 651,544 - -1.2 651,544 - -0.2 651,547 + +0.1 651,547 + -0.3 651,547 + -3.0 651,548 - +0.2 651,548 - +0.2 651,548 - -0.2 651,578 + +0.0 651,578 + -1.6 651,579 - +0.1 651,634 + -0.8 651,634 + +0.1 651,634 + -0.6 651,634 + +0.6 651,634 + +0.1 651,635 - +1.1 651,635 - +0.1 651,635 - -1.6 651,635 - -1.6 651,690 + -1.5 651,691 - +3.0 651,691 - +0.2 651,691 - +0.8
Or see this region's nucleotide sequence