Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5462 and Pf1N1B4_5463 are separated by 281 nucleotides Pf1N1B4_5463 and Pf1N1B4_5464 are separated by 325 nucleotides Pf1N1B4_5464 and Pf1N1B4_5465 are separated by 7 nucleotides
Pf1N1B4_5462 - hypothetical protein, at 209,255 to 210,247
_5462
Pf1N1B4_5463 - hypothetical protein, at 210,529 to 210,654
_5463
Pf1N1B4_5464 - Cointegrate resolution protein T, at 210,980 to 212,032
_5464
Pf1N1B4_5465 - hypothetical protein, at 212,040 to 213,158
_5465
Position (kb) 210
211
212
213 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 209.996 kb on + strand, within Pf1N1B4_5462 at 209.996 kb on + strand at 209.996 kb on + strand, within Pf1N1B4_5462 at 209.997 kb on - strand, within Pf1N1B4_5462 at 209.997 kb on - strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.004 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.079 kb on - strand, within Pf1N1B4_5462 at 210.079 kb on - strand at 210.133 kb on + strand, within Pf1N1B4_5462 at 210.136 kb on + strand, within Pf1N1B4_5462 at 210.136 kb on + strand, within Pf1N1B4_5462 at 210.137 kb on - strand, within Pf1N1B4_5462 at 210.141 kb on + strand, within Pf1N1B4_5462 at 210.146 kb on + strand, within Pf1N1B4_5462 at 210.146 kb on + strand at 210.228 kb on + strand at 210.229 kb on - strand at 210.243 kb on + strand at 210.245 kb on + strand at 210.245 kb on + strand at 210.245 kb on + strand at 210.250 kb on + strand at 210.250 kb on + strand at 210.250 kb on + strand at 210.261 kb on + strand at 210.303 kb on - strand at 210.313 kb on - strand at 210.313 kb on - strand at 210.313 kb on - strand at 210.315 kb on + strand at 210.316 kb on - strand at 210.316 kb on - strand at 210.330 kb on - strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.408 kb on - strand at 210.408 kb on - strand at 210.408 kb on - strand at 210.435 kb on + strand at 210.436 kb on - strand at 210.552 kb on - strand, within Pf1N1B4_5463 at 210.557 kb on + strand at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.690 kb on + strand at 210.759 kb on - strand at 210.760 kb on + strand at 210.761 kb on - strand at 210.761 kb on - strand at 210.761 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.822 kb on + strand at 210.823 kb on - strand at 210.826 kb on + strand at 210.826 kb on + strand at 210.827 kb on - strand at 211.342 kb on - strand, within Pf1N1B4_5464 at 211.342 kb on - strand, within Pf1N1B4_5464 at 211.354 kb on - strand, within Pf1N1B4_5464 at 211.373 kb on + strand, within Pf1N1B4_5464 at 211.458 kb on + strand, within Pf1N1B4_5464 at 211.458 kb on + strand, within Pf1N1B4_5464 at 211.809 kb on + strand, within Pf1N1B4_5464 at 211.809 kb on + strand, within Pf1N1B4_5464 at 211.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 209,996 + Pf1N1B4_5462 0.75 +0.7 209,996 + +0.4 209,996 + Pf1N1B4_5462 0.75 +0.8 209,997 - Pf1N1B4_5462 0.75 -1.0 209,997 - Pf1N1B4_5462 0.75 +1.9 210,000 + Pf1N1B4_5462 0.75 -2.2 210,000 + Pf1N1B4_5462 0.75 -1.1 210,000 + Pf1N1B4_5462 0.75 +0.8 210,000 + Pf1N1B4_5462 0.75 +1.3 210,000 + +0.5 210,000 + Pf1N1B4_5462 0.75 +0.8 210,000 + Pf1N1B4_5462 0.75 +0.2 210,001 - Pf1N1B4_5462 0.75 -0.2 210,001 - Pf1N1B4_5462 0.75 +0.8 210,001 - Pf1N1B4_5462 0.75 +1.0 210,001 - Pf1N1B4_5462 0.75 -0.9 210,004 + Pf1N1B4_5462 0.75 +0.7 210,041 + Pf1N1B4_5462 0.79 +3.2 210,041 + Pf1N1B4_5462 0.79 -2.2 210,041 + Pf1N1B4_5462 0.79 +0.2 210,041 + Pf1N1B4_5462 0.79 -0.8 210,041 + Pf1N1B4_5462 0.79 +0.2 210,042 - Pf1N1B4_5462 0.79 +0.2 210,042 - Pf1N1B4_5462 0.79 -0.4 210,042 - Pf1N1B4_5462 0.79 -2.6 210,042 - Pf1N1B4_5462 0.79 +2.8 210,078 + Pf1N1B4_5462 0.83 -0.8 210,078 + Pf1N1B4_5462 0.83 +1.0 210,078 + Pf1N1B4_5462 0.83 +0.3 210,078 + Pf1N1B4_5462 0.83 -0.6 210,079 - Pf1N1B4_5462 0.83 -2.9 210,079 - -0.2 210,133 + Pf1N1B4_5462 0.88 -2.2 210,136 + Pf1N1B4_5462 0.89 +0.5 210,136 + Pf1N1B4_5462 0.89 -2.2 210,137 - Pf1N1B4_5462 0.89 +1.8 210,141 + Pf1N1B4_5462 0.89 +0.2 210,146 + Pf1N1B4_5462 0.90 +1.4 210,146 + +0.4 210,228 + -1.3 210,229 - +2.5 210,243 + +1.0 210,245 + -0.6 210,245 + -1.1 210,245 + -0.4 210,250 + -2.2 210,250 + +0.2 210,250 + +1.0 210,261 + -0.1 210,303 - -1.2 210,313 - +1.2 210,313 - -0.1 210,313 - +0.8 210,315 + -2.2 210,316 - -0.3 210,316 - +1.0 210,330 - +0.8 210,343 + -1.4 210,343 + +2.2 210,343 + +0.8 210,343 + -0.1 210,343 + -0.2 210,344 - +0.4 210,344 - -0.6 210,344 - -0.6 210,344 - -0.2 210,344 - +0.3 210,344 - +0.8 210,344 - +0.7 210,344 - -1.2 210,344 - -1.8 210,408 - +0.2 210,408 - +0.1 210,408 - -2.1 210,435 + -1.7 210,436 - +0.4 210,552 - Pf1N1B4_5463 0.18 +1.0 210,557 + +0.4 210,566 - Pf1N1B4_5463 0.29 -1.7 210,566 - Pf1N1B4_5463 0.29 -0.8 210,566 - Pf1N1B4_5463 0.29 -2.1 210,566 - Pf1N1B4_5463 0.29 +0.4 210,566 - Pf1N1B4_5463 0.29 +1.2 210,690 + -3.6 210,759 - +0.2 210,760 + -0.1 210,761 - +0.4 210,761 - +2.3 210,761 - -0.2 210,790 - -0.8 210,790 - -2.2 210,790 - -0.8 210,790 - -1.7 210,790 - -1.8 210,822 + +0.8 210,823 - +2.1 210,826 + -1.9 210,826 + -1.1 210,827 - +1.6 211,342 - Pf1N1B4_5464 0.34 -2.1 211,342 - Pf1N1B4_5464 0.34 -1.5 211,354 - Pf1N1B4_5464 0.36 -2.2 211,373 + Pf1N1B4_5464 0.37 -0.2 211,458 + Pf1N1B4_5464 0.45 -1.6 211,458 + Pf1N1B4_5464 0.45 -0.2 211,809 + Pf1N1B4_5464 0.79 +0.5 211,809 + Pf1N1B4_5464 0.79 +2.2 211,943 - +1.8
Or see this region's nucleotide sequence