Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5260 and Pf1N1B4_5261 are separated by 280 nucleotides Pf1N1B4_5261 and Pf1N1B4_5262 are separated by 372 nucleotides Pf1N1B4_5262 and Pf1N1B4_5263 are separated by 491 nucleotides Pf1N1B4_5263 and Pf1N1B4_5264 are separated by 10 nucleotides
Pf1N1B4_5260 - hypothetical protein, at 19,541 to 20,695
_5260
Pf1N1B4_5261 - hypothetical protein, at 20,976 to 21,173
_5261
Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034
_5262
Pf1N1B4_5263 - hypothetical protein, at 22,526 to 22,723
_5263
Pf1N1B4_5264 - hypothetical protein, at 22,734 to 22,868
_5264
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 20.627 kb on + strand at 20.627 kb on + strand at 20.630 kb on + strand at 20.657 kb on + strand at 20.758 kb on + strand at 20.977 kb on + strand at 20.977 kb on + strand at 21.099 kb on + strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.200 kb on + strand at 21.220 kb on + strand at 21.359 kb on + strand at 21.360 kb on - strand at 21.589 kb on + strand at 21.724 kb on + strand, within Pf1N1B4_5262 at 21.725 kb on - strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.943 kb on + strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.961 kb on + strand, within Pf1N1B4_5262 at 22.078 kb on - strand at 22.104 kb on + strand at 22.105 kb on - strand at 22.105 kb on - strand at 22.159 kb on + strand at 22.159 kb on + strand at 22.160 kb on - strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.527 kb on + strand at 22.528 kb on - strand at 22.541 kb on + strand at 22.541 kb on + strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.670 kb on + strand, within Pf1N1B4_5263 at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.671 kb on - strand at 22.671 kb on - strand at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.718 kb on - strand at 22.718 kb on - strand at 22.797 kb on + strand, within Pf1N1B4_5264 at 22.798 kb on - strand at 22.878 kb on + strand at 22.879 kb on - strand at 22.879 kb on - strand at 22.905 kb on - strand at 22.906 kb on + strand at 22.907 kb on - strand at 22.907 kb on - strand at 22.938 kb on - strand at 22.958 kb on - strand at 22.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 20,627 + -1.6 20,627 + -0.5 20,630 + +3.3 20,657 + -0.2 20,758 + +0.2 20,977 + -0.2 20,977 + +1.8 21,099 + Pf1N1B4_5261 0.62 -0.8 21,100 - Pf1N1B4_5261 0.63 +1.2 21,100 - Pf1N1B4_5261 0.63 -1.6 21,100 - Pf1N1B4_5261 0.63 -0.2 21,200 + -3.1 21,220 + +0.5 21,359 + +0.8 21,360 - -0.6 21,589 + -0.4 21,724 + Pf1N1B4_5262 0.36 -0.9 21,725 - Pf1N1B4_5262 0.37 -1.0 21,904 + Pf1N1B4_5262 0.73 -0.7 21,904 + Pf1N1B4_5262 0.73 -2.4 21,904 + Pf1N1B4_5262 0.73 -1.7 21,905 - Pf1N1B4_5262 0.73 +1.8 21,905 - Pf1N1B4_5262 0.73 -1.6 21,905 - Pf1N1B4_5262 0.73 -0.3 21,905 - Pf1N1B4_5262 0.73 +1.8 21,941 + Pf1N1B4_5262 0.81 -2.9 21,941 + Pf1N1B4_5262 0.81 +0.0 21,943 + Pf1N1B4_5262 0.81 -1.4 21,944 - Pf1N1B4_5262 0.81 +0.3 21,944 - Pf1N1B4_5262 0.81 -1.1 21,961 + Pf1N1B4_5262 0.85 -0.8 22,078 - +0.0 22,104 + -2.1 22,105 - -0.2 22,105 - +0.2 22,159 + -1.4 22,159 + +0.3 22,160 - -1.0 22,162 + -2.1 22,162 + -1.1 22,162 + -2.7 22,162 + +0.8 22,162 + -1.4 22,527 + +1.2 22,528 - -0.6 22,541 + +1.4 22,541 + -0.2 22,542 - -1.2 22,542 - -1.9 22,542 - -1.6 22,542 - -0.7 22,604 + Pf1N1B4_5263 0.39 -0.2 22,604 + Pf1N1B4_5263 0.39 +0.8 22,605 - Pf1N1B4_5263 0.40 -0.3 22,605 - Pf1N1B4_5263 0.40 +1.2 22,605 - Pf1N1B4_5263 0.40 +0.9 22,605 - Pf1N1B4_5263 0.40 +0.9 22,605 - Pf1N1B4_5263 0.40 -0.2 22,605 - Pf1N1B4_5263 0.40 -2.4 22,605 - Pf1N1B4_5263 0.40 -0.4 22,605 - Pf1N1B4_5263 0.40 -1.7 22,605 - Pf1N1B4_5263 0.40 -0.8 22,605 - Pf1N1B4_5263 0.40 +0.9 22,670 + Pf1N1B4_5263 0.73 +1.5 22,671 - Pf1N1B4_5263 0.73 +1.8 22,671 - -0.6 22,671 - -1.2 22,671 - Pf1N1B4_5263 0.73 -0.1 22,718 - -1.4 22,718 - +0.3 22,797 + Pf1N1B4_5264 0.47 +2.8 22,798 - +1.2 22,878 + -1.3 22,879 - +1.3 22,879 - -0.6 22,905 - -0.8 22,906 + -1.8 22,907 - +1.0 22,907 - -1.8 22,938 - +1.8 22,958 - +0.8 22,974 - -1.1
Or see this region's nucleotide sequence