Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5262

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5260 and Pf1N1B4_5261 are separated by 280 nucleotidesPf1N1B4_5261 and Pf1N1B4_5262 are separated by 372 nucleotidesPf1N1B4_5262 and Pf1N1B4_5263 are separated by 491 nucleotidesPf1N1B4_5263 and Pf1N1B4_5264 are separated by 10 nucleotides Pf1N1B4_5260 - hypothetical protein, at 19,541 to 20,695 _5260 Pf1N1B4_5261 - hypothetical protein, at 20,976 to 21,173 _5261 Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034 _5262 Pf1N1B4_5263 - hypothetical protein, at 22,526 to 22,723 _5263 Pf1N1B4_5264 - hypothetical protein, at 22,734 to 22,868 _5264 Position (kb) 21 22 23Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 20.627 kb on + strandat 20.627 kb on + strandat 20.630 kb on + strandat 20.657 kb on + strandat 20.758 kb on + strandat 20.977 kb on + strandat 20.977 kb on + strandat 21.099 kb on + strand, within Pf1N1B4_5261at 21.100 kb on - strand, within Pf1N1B4_5261at 21.100 kb on - strand, within Pf1N1B4_5261at 21.100 kb on - strand, within Pf1N1B4_5261at 21.200 kb on + strandat 21.220 kb on + strandat 21.359 kb on + strandat 21.360 kb on - strandat 21.589 kb on + strandat 21.724 kb on + strand, within Pf1N1B4_5262at 21.725 kb on - strand, within Pf1N1B4_5262at 21.904 kb on + strand, within Pf1N1B4_5262at 21.904 kb on + strand, within Pf1N1B4_5262at 21.904 kb on + strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.941 kb on + strand, within Pf1N1B4_5262at 21.941 kb on + strand, within Pf1N1B4_5262at 21.943 kb on + strand, within Pf1N1B4_5262at 21.944 kb on - strand, within Pf1N1B4_5262at 21.944 kb on - strand, within Pf1N1B4_5262at 21.961 kb on + strand, within Pf1N1B4_5262at 22.078 kb on - strandat 22.104 kb on + strandat 22.105 kb on - strandat 22.105 kb on - strandat 22.159 kb on + strandat 22.159 kb on + strandat 22.160 kb on - strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.527 kb on + strandat 22.528 kb on - strandat 22.541 kb on + strandat 22.541 kb on + strandat 22.542 kb on - strandat 22.542 kb on - strandat 22.542 kb on - strandat 22.542 kb on - strandat 22.604 kb on + strand, within Pf1N1B4_5263at 22.604 kb on + strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.670 kb on + strand, within Pf1N1B4_5263at 22.671 kb on - strand, within Pf1N1B4_5263at 22.671 kb on - strandat 22.671 kb on - strandat 22.671 kb on - strand, within Pf1N1B4_5263at 22.718 kb on - strandat 22.718 kb on - strandat 22.797 kb on + strand, within Pf1N1B4_5264at 22.798 kb on - strandat 22.878 kb on + strandat 22.879 kb on - strandat 22.879 kb on - strandat 22.905 kb on - strandat 22.906 kb on + strandat 22.907 kb on - strandat 22.907 kb on - strandat 22.938 kb on - strandat 22.958 kb on - strandat 22.974 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
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20,627 + -1.6
20,627 + -0.5
20,630 + +3.3
20,657 + -0.2
20,758 + +0.2
20,977 + -0.2
20,977 + +1.8
21,099 + Pf1N1B4_5261 0.62 -0.8
21,100 - Pf1N1B4_5261 0.63 +1.2
21,100 - Pf1N1B4_5261 0.63 -1.6
21,100 - Pf1N1B4_5261 0.63 -0.2
21,200 + -3.1
21,220 + +0.5
21,359 + +0.8
21,360 - -0.6
21,589 + -0.4
21,724 + Pf1N1B4_5262 0.36 -0.9
21,725 - Pf1N1B4_5262 0.37 -1.0
21,904 + Pf1N1B4_5262 0.73 -0.7
21,904 + Pf1N1B4_5262 0.73 -2.4
21,904 + Pf1N1B4_5262 0.73 -1.7
21,905 - Pf1N1B4_5262 0.73 +1.8
21,905 - Pf1N1B4_5262 0.73 -1.6
21,905 - Pf1N1B4_5262 0.73 -0.3
21,905 - Pf1N1B4_5262 0.73 +1.8
21,941 + Pf1N1B4_5262 0.81 -2.9
21,941 + Pf1N1B4_5262 0.81 +0.0
21,943 + Pf1N1B4_5262 0.81 -1.4
21,944 - Pf1N1B4_5262 0.81 +0.3
21,944 - Pf1N1B4_5262 0.81 -1.1
21,961 + Pf1N1B4_5262 0.85 -0.8
22,078 - +0.0
22,104 + -2.1
22,105 - -0.2
22,105 - +0.2
22,159 + -1.4
22,159 + +0.3
22,160 - -1.0
22,162 + -2.1
22,162 + -1.1
22,162 + -2.7
22,162 + +0.8
22,162 + -1.4
22,527 + +1.2
22,528 - -0.6
22,541 + +1.4
22,541 + -0.2
22,542 - -1.2
22,542 - -1.9
22,542 - -1.6
22,542 - -0.7
22,604 + Pf1N1B4_5263 0.39 -0.2
22,604 + Pf1N1B4_5263 0.39 +0.8
22,605 - Pf1N1B4_5263 0.40 -0.3
22,605 - Pf1N1B4_5263 0.40 +1.2
22,605 - Pf1N1B4_5263 0.40 +0.9
22,605 - Pf1N1B4_5263 0.40 +0.9
22,605 - Pf1N1B4_5263 0.40 -0.2
22,605 - Pf1N1B4_5263 0.40 -2.4
22,605 - Pf1N1B4_5263 0.40 -0.4
22,605 - Pf1N1B4_5263 0.40 -1.7
22,605 - Pf1N1B4_5263 0.40 -0.8
22,605 - Pf1N1B4_5263 0.40 +0.9
22,670 + Pf1N1B4_5263 0.73 +1.5
22,671 - Pf1N1B4_5263 0.73 +1.8
22,671 - -0.6
22,671 - -1.2
22,671 - Pf1N1B4_5263 0.73 -0.1
22,718 - -1.4
22,718 - +0.3
22,797 + Pf1N1B4_5264 0.47 +2.8
22,798 - +1.2
22,878 + -1.3
22,879 - +1.3
22,879 - -0.6
22,905 - -0.8
22,906 + -1.8
22,907 - +1.0
22,907 - -1.8
22,938 - +1.8
22,958 - +0.8
22,974 - -1.1

Or see this region's nucleotide sequence