Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4955 and Pf1N1B4_4956 are separated by 159 nucleotides
Pf1N1B4_4955 - Methyltransferase, at 5,494,872 to 5,495,744
_4955
Pf1N1B4_4956 - Thioredoxin reductase (EC 1.8.1.9), at 5,495,904 to 5,496,797
_4956
Position (kb)
5494
5495
5496 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5493.951 kb on + strand at 5493.952 kb on - strand at 5493.954 kb on + strand at 5494.329 kb on + strand at 5494.329 kb on + strand at 5494.331 kb on + strand at 5494.428 kb on + strand at 5494.428 kb on + strand at 5494.473 kb on + strand at 5494.522 kb on - strand at 5494.551 kb on + strand at 5494.554 kb on + strand at 5494.604 kb on + strand at 5494.640 kb on + strand at 5494.645 kb on - strand at 5494.750 kb on - strand at 5494.753 kb on + strand at 5494.754 kb on - strand at 5494.819 kb on + strand at 5494.828 kb on - strand at 5495.021 kb on + strand, within Pf1N1B4_4955 at 5495.021 kb on + strand, within Pf1N1B4_4955 at 5495.021 kb on + strand, within Pf1N1B4_4955 at 5495.022 kb on - strand, within Pf1N1B4_4955 at 5495.022 kb on - strand, within Pf1N1B4_4955 at 5495.022 kb on - strand, within Pf1N1B4_4955 at 5495.022 kb on - strand, within Pf1N1B4_4955 at 5495.022 kb on - strand, within Pf1N1B4_4955 at 5495.022 kb on - strand, within Pf1N1B4_4955 at 5495.159 kb on + strand, within Pf1N1B4_4955 at 5495.160 kb on - strand, within Pf1N1B4_4955 at 5495.397 kb on + strand, within Pf1N1B4_4955 at 5495.397 kb on + strand, within Pf1N1B4_4955 at 5495.397 kb on + strand, within Pf1N1B4_4955 at 5495.398 kb on - strand, within Pf1N1B4_4955 at 5495.456 kb on + strand, within Pf1N1B4_4955 at 5495.456 kb on + strand, within Pf1N1B4_4955 at 5495.508 kb on + strand, within Pf1N1B4_4955 at 5495.508 kb on + strand, within Pf1N1B4_4955 at 5495.509 kb on - strand, within Pf1N1B4_4955 at 5495.523 kb on + strand, within Pf1N1B4_4955 at 5495.771 kb on + strand at 5495.772 kb on - strand at 5495.772 kb on - strand at 5495.776 kb on - strand at 5495.776 kb on - strand at 5495.776 kb on - strand at 5495.776 kb on - strand at 5495.776 kb on - strand at 5495.779 kb on + strand at 5495.790 kb on + strand at 5495.791 kb on - strand at 5495.791 kb on - strand at 5495.849 kb on + strand at 5495.849 kb on + strand at 5496.007 kb on + strand, within Pf1N1B4_4956 at 5496.008 kb on - strand, within Pf1N1B4_4956 at 5496.325 kb on - strand, within Pf1N1B4_4956 at 5496.325 kb on - strand, within Pf1N1B4_4956 at 5496.334 kb on + strand at 5496.334 kb on + strand, within Pf1N1B4_4956 at 5496.334 kb on + strand, within Pf1N1B4_4956 at 5496.334 kb on + strand, within Pf1N1B4_4956 at 5496.334 kb on + strand, within Pf1N1B4_4956 at 5496.334 kb on + strand, within Pf1N1B4_4956 at 5496.334 kb on + strand, within Pf1N1B4_4956 at 5496.334 kb on + strand at 5496.335 kb on - strand, within Pf1N1B4_4956 at 5496.557 kb on + strand, within Pf1N1B4_4956 at 5496.558 kb on - strand, within Pf1N1B4_4956 at 5496.558 kb on - strand, within Pf1N1B4_4956 at 5496.591 kb on - strand, within Pf1N1B4_4956 at 5496.661 kb on - strand, within Pf1N1B4_4956
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 5,493,951 + -0.8 5,493,952 - +1.4 5,493,954 + -1.1 5,494,329 + +0.0 5,494,329 + -0.2 5,494,331 + -0.3 5,494,428 + -0.3 5,494,428 + +0.8 5,494,473 + -2.3 5,494,522 - -0.2 5,494,551 + +0.0 5,494,554 + -0.3 5,494,604 + +0.8 5,494,640 + -1.8 5,494,645 - -1.3 5,494,750 - -1.0 5,494,753 + -1.5 5,494,754 - +0.4 5,494,819 + -0.6 5,494,828 - -1.4 5,495,021 + Pf1N1B4_4955 0.17 -0.6 5,495,021 + Pf1N1B4_4955 0.17 +0.7 5,495,021 + Pf1N1B4_4955 0.17 -0.4 5,495,022 - Pf1N1B4_4955 0.17 +0.4 5,495,022 - Pf1N1B4_4955 0.17 -0.8 5,495,022 - Pf1N1B4_4955 0.17 -2.2 5,495,022 - Pf1N1B4_4955 0.17 +0.1 5,495,022 - Pf1N1B4_4955 0.17 -1.1 5,495,022 - Pf1N1B4_4955 0.17 +1.6 5,495,159 + Pf1N1B4_4955 0.33 -0.5 5,495,160 - Pf1N1B4_4955 0.33 +0.3 5,495,397 + Pf1N1B4_4955 0.60 +3.9 5,495,397 + Pf1N1B4_4955 0.60 -0.2 5,495,397 + Pf1N1B4_4955 0.60 +2.2 5,495,398 - Pf1N1B4_4955 0.60 +0.4 5,495,456 + Pf1N1B4_4955 0.67 +0.8 5,495,456 + Pf1N1B4_4955 0.67 -1.5 5,495,508 + Pf1N1B4_4955 0.73 -0.8 5,495,508 + Pf1N1B4_4955 0.73 +0.7 5,495,509 - Pf1N1B4_4955 0.73 -1.3 5,495,523 + Pf1N1B4_4955 0.75 -0.9 5,495,771 + +1.5 5,495,772 - -2.3 5,495,772 - -1.3 5,495,776 - -0.2 5,495,776 - -1.9 5,495,776 - -0.5 5,495,776 - -0.7 5,495,776 - -1.1 5,495,779 + -1.3 5,495,790 + -1.2 5,495,791 - +0.6 5,495,791 - -1.8 5,495,849 + -0.2 5,495,849 + +0.3 5,496,007 + Pf1N1B4_4956 0.12 +1.3 5,496,008 - Pf1N1B4_4956 0.12 +0.8 5,496,325 - Pf1N1B4_4956 0.47 +0.2 5,496,325 - Pf1N1B4_4956 0.47 +1.5 5,496,334 + -0.5 5,496,334 + Pf1N1B4_4956 0.48 -0.2 5,496,334 + Pf1N1B4_4956 0.48 +3.6 5,496,334 + Pf1N1B4_4956 0.48 -0.2 5,496,334 + Pf1N1B4_4956 0.48 -1.0 5,496,334 + Pf1N1B4_4956 0.48 +0.3 5,496,334 + Pf1N1B4_4956 0.48 +1.3 5,496,334 + -0.5 5,496,335 - Pf1N1B4_4956 0.48 -0.1 5,496,557 + Pf1N1B4_4956 0.73 -0.6 5,496,558 - Pf1N1B4_4956 0.73 -1.0 5,496,558 - Pf1N1B4_4956 0.73 +0.5 5,496,591 - Pf1N1B4_4956 0.77 +0.5 5,496,661 - Pf1N1B4_4956 0.85 +1.6
Or see this region's nucleotide sequence