Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_491 and Pf1N1B4_492 overlap by 4 nucleotides Pf1N1B4_492 and Pf1N1B4_493 are separated by 18 nucleotides Pf1N1B4_493 and Pf1N1B4_494 are separated by 735 nucleotides
Pf1N1B4_491 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 583,248 to 584,471
_491
Pf1N1B4_492 - Flp pilus assembly protein TadD, contains TPR repeats, at 584,468 to 585,295
_492
Pf1N1B4_493 - hypothetical protein, at 585,314 to 585,583
_493
Pf1N1B4_494 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 586,319 to 587,281
_494
Position (kb) 585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 584.365 kb on + strand at 584.365 kb on + strand at 584.365 kb on + strand at 584.366 kb on - strand at 584.366 kb on - strand at 584.366 kb on - strand at 584.397 kb on + strand at 584.398 kb on - strand at 584.398 kb on - strand at 584.569 kb on - strand, within Pf1N1B4_492 at 584.663 kb on - strand, within Pf1N1B4_492 at 584.738 kb on + strand, within Pf1N1B4_492 at 584.739 kb on - strand, within Pf1N1B4_492 at 584.846 kb on + strand, within Pf1N1B4_492 at 584.846 kb on + strand, within Pf1N1B4_492 at 584.847 kb on - strand, within Pf1N1B4_492 at 585.008 kb on + strand, within Pf1N1B4_492 at 585.009 kb on - strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.026 kb on - strand, within Pf1N1B4_492 at 585.098 kb on - strand, within Pf1N1B4_492 at 585.116 kb on + strand, within Pf1N1B4_492 at 585.117 kb on - strand at 585.117 kb on - strand, within Pf1N1B4_492 at 585.254 kb on - strand at 585.254 kb on - strand at 585.293 kb on + strand at 585.294 kb on - strand at 585.294 kb on - strand at 585.294 kb on - strand at 585.301 kb on - strand at 585.319 kb on + strand at 585.429 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.619 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.664 kb on + strand at 585.665 kb on - strand at 585.665 kb on - strand at 585.710 kb on + strand at 585.799 kb on + strand at 585.799 kb on + strand at 585.913 kb on + strand at 585.927 kb on + strand at 585.927 kb on + strand at 585.927 kb on + strand at 585.928 kb on - strand at 586.012 kb on + strand at 586.012 kb on + strand at 586.013 kb on - strand at 586.034 kb on + strand at 586.034 kb on + strand at 586.035 kb on - strand at 586.035 kb on - strand at 586.124 kb on + strand at 586.124 kb on + strand at 586.124 kb on + strand at 586.124 kb on + strand at 586.125 kb on - strand at 586.125 kb on - strand at 586.125 kb on - strand at 586.125 kb on - strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.127 kb on - strand at 586.127 kb on - strand at 586.127 kb on - strand at 586.127 kb on - strand at 586.140 kb on + strand at 586.235 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.251 kb on + strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.252 kb on - strand at 586.294 kb on + strand at 586.294 kb on + strand at 586.406 kb on + strand at 586.407 kb on - strand at 586.407 kb on - strand at 586.453 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.479 kb on + strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.480 kb on - strand, within Pf1N1B4_494 at 586.520 kb on - strand, within Pf1N1B4_494 at 586.550 kb on + strand, within Pf1N1B4_494 at 586.550 kb on + strand, within Pf1N1B4_494 at 586.550 kb on + strand, within Pf1N1B4_494 at 586.551 kb on - strand at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494 at 586.551 kb on - strand, within Pf1N1B4_494
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 584,365 + +0.8 584,365 + -0.1 584,365 + +0.2 584,366 - -1.4 584,366 - -2.0 584,366 - -0.7 584,397 + +0.8 584,398 - -2.5 584,398 - -2.2 584,569 - Pf1N1B4_492 0.12 -0.0 584,663 - Pf1N1B4_492 0.24 +0.2 584,738 + Pf1N1B4_492 0.33 -1.8 584,739 - Pf1N1B4_492 0.33 +0.5 584,846 + Pf1N1B4_492 0.46 +1.3 584,846 + Pf1N1B4_492 0.46 -1.0 584,847 - Pf1N1B4_492 0.46 -0.6 585,008 + Pf1N1B4_492 0.65 -2.5 585,009 - Pf1N1B4_492 0.65 -2.7 585,025 + Pf1N1B4_492 0.67 +0.5 585,025 + Pf1N1B4_492 0.67 +0.4 585,025 + Pf1N1B4_492 0.67 +1.6 585,026 - Pf1N1B4_492 0.67 +0.6 585,098 - Pf1N1B4_492 0.76 -0.0 585,116 + Pf1N1B4_492 0.78 +0.2 585,117 - -1.7 585,117 - Pf1N1B4_492 0.78 +0.3 585,254 - -0.8 585,254 - -1.2 585,293 + -0.7 585,294 - -0.5 585,294 - -0.8 585,294 - -0.2 585,301 - -1.0 585,319 + -0.0 585,429 + Pf1N1B4_493 0.43 +0.0 585,444 + Pf1N1B4_493 0.48 +0.2 585,444 + Pf1N1B4_493 0.48 -1.0 585,444 + Pf1N1B4_493 0.48 +2.4 585,444 + +0.8 585,444 + Pf1N1B4_493 0.48 +0.5 585,444 + Pf1N1B4_493 0.48 +3.2 585,445 - Pf1N1B4_493 0.49 -2.1 585,445 - Pf1N1B4_493 0.49 +0.7 585,445 - Pf1N1B4_493 0.49 -0.8 585,619 + +1.4 585,664 + -1.9 585,664 + +2.6 585,664 + +0.6 585,664 + -1.2 585,664 + +1.8 585,665 - -0.5 585,665 - +1.8 585,710 + -0.8 585,799 + +1.5 585,799 + -0.5 585,913 + +0.2 585,927 + -0.2 585,927 + +0.5 585,927 + -0.2 585,928 - +1.8 586,012 + -1.2 586,012 + -0.2 586,013 - -2.7 586,034 + +1.8 586,034 + +3.4 586,035 - -0.5 586,035 - -0.9 586,124 + +3.5 586,124 + -0.8 586,124 + -3.0 586,124 + +1.8 586,125 - -0.2 586,125 - -0.4 586,125 - +1.6 586,125 - -0.2 586,126 + +0.2 586,126 + -1.9 586,126 + +0.6 586,126 + +0.2 586,126 + +1.5 586,126 + +1.4 586,126 + -0.8 586,126 + -1.0 586,126 + +0.6 586,127 - +2.8 586,127 - -0.8 586,127 - -0.4 586,127 - -0.8 586,140 + +1.4 586,235 + +1.8 586,251 + -0.6 586,251 + +1.2 586,251 + -0.2 586,251 + -0.1 586,251 + +1.5 586,251 + +2.4 586,251 + -0.9 586,251 + +0.1 586,251 + -1.3 586,251 + -1.5 586,251 + +0.9 586,252 - -2.6 586,252 - -0.8 586,252 - -1.7 586,252 - +0.9 586,252 - +1.8 586,252 - -1.2 586,252 - -1.0 586,294 + -1.0 586,294 + -0.5 586,406 + +0.1 586,407 - +2.3 586,407 - -0.4 586,453 + Pf1N1B4_494 0.14 -0.3 586,479 + Pf1N1B4_494 0.17 -1.5 586,479 + Pf1N1B4_494 0.17 -2.1 586,479 + Pf1N1B4_494 0.17 -0.5 586,479 + Pf1N1B4_494 0.17 +2.5 586,480 - Pf1N1B4_494 0.17 -0.1 586,480 - Pf1N1B4_494 0.17 +2.8 586,480 - Pf1N1B4_494 0.17 +1.0 586,480 - Pf1N1B4_494 0.17 -0.5 586,480 - Pf1N1B4_494 0.17 -1.5 586,520 - Pf1N1B4_494 0.21 -0.0 586,550 + Pf1N1B4_494 0.24 +0.3 586,550 + Pf1N1B4_494 0.24 -1.0 586,550 + Pf1N1B4_494 0.24 -0.0 586,551 - +1.8 586,551 - Pf1N1B4_494 0.24 -2.1 586,551 - Pf1N1B4_494 0.24 -1.1 586,551 - Pf1N1B4_494 0.24 -1.4 586,551 - Pf1N1B4_494 0.24 -0.8 586,551 - Pf1N1B4_494 0.24 -1.5 586,551 - Pf1N1B4_494 0.24 -0.8
Or see this region's nucleotide sequence