Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_480 and Pf1N1B4_481 are separated by 100 nucleotides Pf1N1B4_481 and Pf1N1B4_482 are separated by 196 nucleotides Pf1N1B4_482 and Pf1N1B4_483 are separated by 135 nucleotides Pf1N1B4_483 and Pf1N1B4_484 are separated by 92 nucleotides
Pf1N1B4_480 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 570,930 to 573,119
_480
Pf1N1B4_481 - FIG00956241: hypothetical protein, at 573,220 to 573,627
_481
Pf1N1B4_482 - Transcriptional regulator, LysR family, at 573,824 to 574,822
_482
Pf1N1B4_483 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 574,958 to 575,539
_483
Pf1N1B4_484 - Hydrogenase maturation factor, at 575,632 to 576,402
_484
Position (kb) 573
574
575 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 573.095 kb on - strand at 573.153 kb on + strand at 573.153 kb on + strand at 573.153 kb on + strand at 573.154 kb on - strand at 573.154 kb on - strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.181 kb on + strand at 573.181 kb on + strand at 573.181 kb on + strand at 573.182 kb on - strand at 573.182 kb on - strand at 573.183 kb on + strand at 573.183 kb on + strand at 573.183 kb on + strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.284 kb on + strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.386 kb on - strand, within Pf1N1B4_481 at 573.466 kb on + strand, within Pf1N1B4_481 at 573.466 kb on + strand, within Pf1N1B4_481 at 573.466 kb on + strand at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.491 kb on - strand at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.517 kb on + strand, within Pf1N1B4_481 at 573.593 kb on + strand at 573.662 kb on + strand at 573.663 kb on - strand at 573.855 kb on + strand at 573.855 kb on + strand at 573.855 kb on + strand at 573.856 kb on - strand at 573.856 kb on - strand at 573.937 kb on - strand, within Pf1N1B4_482 at 574.083 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.727 kb on + strand at 574.727 kb on + strand at 574.728 kb on - strand at 574.728 kb on - strand at 574.728 kb on - strand at 574.882 kb on - strand at 574.888 kb on - strand at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.452 kb on - strand, within Pf1N1B4_483 at 575.526 kb on - strand at 575.526 kb on - strand at 575.526 kb on - strand at 575.526 kb on - strand at 575.615 kb on + strand at 575.615 kb on + strand at 575.615 kb on + strand at 575.625 kb on + strand at 575.625 kb on + strand at 575.625 kb on + strand at 575.626 kb on - strand at 575.669 kb on + strand at 575.669 kb on + strand at 575.669 kb on + strand at 575.670 kb on - strand at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.805 kb on + strand, within Pf1N1B4_484 at 575.805 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.822 kb on - strand, within Pf1N1B4_484
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 573,095 - -0.0 573,153 + -1.3 573,153 + -1.8 573,153 + -1.9 573,154 - -1.7 573,154 - -0.5 573,156 + -0.4 573,156 + +0.8 573,156 + +3.8 573,156 + +0.8 573,156 + +3.0 573,156 + +0.5 573,157 - +1.5 573,157 - +2.0 573,157 - +0.6 573,157 - +1.8 573,157 - -1.0 573,157 - -0.3 573,157 - +2.4 573,181 + +0.2 573,181 + +0.6 573,181 + +0.8 573,182 - +0.5 573,182 - +0.0 573,183 + -1.8 573,183 + -2.3 573,183 + -1.9 573,184 - -0.2 573,184 - -0.5 573,184 - +0.5 573,184 - -1.6 573,184 - -0.7 573,214 + +0.2 573,214 + +1.8 573,214 + +0.8 573,214 + +0.4 573,215 - -0.9 573,215 - -1.8 573,215 - +1.7 573,215 - -0.5 573,284 + Pf1N1B4_481 0.16 -1.0 573,285 - Pf1N1B4_481 0.16 -1.0 573,285 - Pf1N1B4_481 0.16 +0.8 573,285 - Pf1N1B4_481 0.16 +1.0 573,386 - Pf1N1B4_481 0.41 +1.8 573,466 + Pf1N1B4_481 0.60 +0.8 573,466 + Pf1N1B4_481 0.60 +1.3 573,466 + +0.8 573,490 + Pf1N1B4_481 0.66 +0.6 573,490 + Pf1N1B4_481 0.66 +0.2 573,490 + Pf1N1B4_481 0.66 -0.2 573,490 + Pf1N1B4_481 0.66 -1.2 573,490 + Pf1N1B4_481 0.66 -0.1 573,490 + Pf1N1B4_481 0.66 -0.1 573,490 + Pf1N1B4_481 0.66 +2.5 573,490 + Pf1N1B4_481 0.66 -0.5 573,491 - -0.5 573,491 - Pf1N1B4_481 0.66 +1.3 573,491 - Pf1N1B4_481 0.66 +0.8 573,491 - Pf1N1B4_481 0.66 -0.0 573,491 - Pf1N1B4_481 0.66 -2.0 573,491 - Pf1N1B4_481 0.66 -0.7 573,491 - Pf1N1B4_481 0.66 +0.1 573,517 + Pf1N1B4_481 0.73 +2.0 573,593 + -1.2 573,662 + -0.3 573,663 - -2.2 573,855 + +0.2 573,855 + +0.5 573,855 + +2.2 573,856 - +1.3 573,856 - +1.1 573,937 - Pf1N1B4_482 0.11 +2.0 574,083 + Pf1N1B4_482 0.26 -0.5 574,233 + Pf1N1B4_482 0.41 +1.4 574,233 + Pf1N1B4_482 0.41 +0.0 574,234 - Pf1N1B4_482 0.41 +0.2 574,234 - Pf1N1B4_482 0.41 -0.8 574,237 + Pf1N1B4_482 0.41 -0.8 574,237 + Pf1N1B4_482 0.41 +1.4 574,238 - Pf1N1B4_482 0.41 -0.4 574,238 - Pf1N1B4_482 0.41 -0.5 574,238 - Pf1N1B4_482 0.41 +1.2 574,238 - Pf1N1B4_482 0.41 +1.8 574,238 - Pf1N1B4_482 0.41 -0.5 574,238 - Pf1N1B4_482 0.41 +1.4 574,238 - Pf1N1B4_482 0.41 -0.4 574,238 - Pf1N1B4_482 0.41 -0.2 574,313 + Pf1N1B4_482 0.49 -0.7 574,313 + Pf1N1B4_482 0.49 -1.4 574,314 - Pf1N1B4_482 0.49 -0.1 574,314 - Pf1N1B4_482 0.49 -1.0 574,314 - Pf1N1B4_482 0.49 +1.0 574,727 + +0.5 574,727 + +1.4 574,728 - +0.4 574,728 - -0.5 574,728 - +1.8 574,882 - +1.1 574,888 - -1.9 575,451 + Pf1N1B4_483 0.85 -0.5 575,451 + Pf1N1B4_483 0.85 -0.7 575,451 + Pf1N1B4_483 0.85 +2.8 575,451 + Pf1N1B4_483 0.85 +1.2 575,452 - Pf1N1B4_483 0.85 +0.4 575,526 - +1.0 575,526 - -2.5 575,526 - +1.2 575,526 - -1.1 575,615 + +0.8 575,615 + +2.4 575,615 + -0.6 575,625 + -2.3 575,625 + +1.8 575,625 + -2.6 575,626 - +1.4 575,669 + +0.8 575,669 + -0.2 575,669 + +0.0 575,670 - -1.9 575,779 + Pf1N1B4_484 0.19 -0.2 575,779 + Pf1N1B4_484 0.19 +1.4 575,779 + Pf1N1B4_484 0.19 -1.1 575,779 + Pf1N1B4_484 0.19 -1.3 575,779 + Pf1N1B4_484 0.19 -0.2 575,780 - Pf1N1B4_484 0.19 +1.0 575,780 - Pf1N1B4_484 0.19 +0.8 575,780 - Pf1N1B4_484 0.19 -0.2 575,805 + Pf1N1B4_484 0.22 +0.8 575,805 + Pf1N1B4_484 0.22 -1.7 575,821 + Pf1N1B4_484 0.25 +0.9 575,821 + Pf1N1B4_484 0.25 +0.4 575,821 + Pf1N1B4_484 0.25 -1.1 575,822 - Pf1N1B4_484 0.25 +0.2
Or see this region's nucleotide sequence