Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4711 and Pf1N1B4_4712 are separated by 3 nucleotides Pf1N1B4_4712 and Pf1N1B4_4713 are separated by 55 nucleotides Pf1N1B4_4713 and Pf1N1B4_4714 overlap by 47 nucleotides Pf1N1B4_4714 and Pf1N1B4_4715 are separated by 175 nucleotides
Pf1N1B4_4711 - hypothetical protein, at 5,207,898 to 5,209,475
_4711
Pf1N1B4_4712 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,209,479 to 5,209,952
_4712
Pf1N1B4_4713 - Xanthine permease, at 5,210,008 to 5,211,483
_4713
Pf1N1B4_4714 - hypothetical protein, at 5,211,437 to 5,211,553
_4714
Pf1N1B4_4715 - Transcriptional regulator, at 5,211,729 to 5,212,622
_4715
Position (kb)
5210
5211
5212 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5209.186 kb on - strand at 5209.281 kb on + strand, within Pf1N1B4_4711 at 5209.411 kb on + strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.579 kb on + strand, within Pf1N1B4_4712 at 5209.579 kb on + strand, within Pf1N1B4_4712 at 5209.579 kb on + strand, within Pf1N1B4_4712 at 5209.580 kb on - strand, within Pf1N1B4_4712 at 5209.765 kb on + strand at 5210.021 kb on - strand at 5210.154 kb on + strand at 5210.155 kb on - strand at 5210.155 kb on - strand at 5210.236 kb on + strand, within Pf1N1B4_4713 at 5210.606 kb on + strand, within Pf1N1B4_4713 at 5210.606 kb on + strand, within Pf1N1B4_4713 at 5210.606 kb on + strand, within Pf1N1B4_4713 at 5210.606 kb on + strand, within Pf1N1B4_4713 at 5210.606 kb on + strand, within Pf1N1B4_4713 at 5210.607 kb on - strand, within Pf1N1B4_4713 at 5210.607 kb on - strand, within Pf1N1B4_4713 at 5210.607 kb on - strand at 5210.607 kb on - strand, within Pf1N1B4_4713 at 5210.607 kb on - strand, within Pf1N1B4_4713 at 5210.607 kb on - strand, within Pf1N1B4_4713 at 5211.299 kb on + strand, within Pf1N1B4_4713 at 5211.376 kb on + strand at 5211.376 kb on + strand at 5211.377 kb on - strand at 5211.377 kb on - strand at 5211.562 kb on + strand at 5211.563 kb on - strand at 5211.563 kb on - strand at 5211.563 kb on - strand at 5211.727 kb on - strand at 5211.727 kb on - strand at 5211.727 kb on - strand at 5212.091 kb on - strand, within Pf1N1B4_4715 at 5212.154 kb on - strand, within Pf1N1B4_4715 at 5212.154 kb on - strand, within Pf1N1B4_4715
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 5,209,186 - -1.6 5,209,281 + Pf1N1B4_4711 0.88 +0.5 5,209,411 + +0.2 5,209,412 - -1.1 5,209,412 - -0.6 5,209,412 - -0.9 5,209,412 - -0.3 5,209,412 - +1.7 5,209,412 - +0.4 5,209,412 - -1.5 5,209,579 + Pf1N1B4_4712 0.21 +1.1 5,209,579 + Pf1N1B4_4712 0.21 -0.1 5,209,579 + Pf1N1B4_4712 0.21 -1.7 5,209,580 - Pf1N1B4_4712 0.21 -0.1 5,209,765 + +0.7 5,210,021 - +0.1 5,210,154 + -1.9 5,210,155 - -0.3 5,210,155 - +0.2 5,210,236 + Pf1N1B4_4713 0.15 -2.7 5,210,606 + Pf1N1B4_4713 0.41 -0.4 5,210,606 + Pf1N1B4_4713 0.41 +1.1 5,210,606 + Pf1N1B4_4713 0.41 -0.6 5,210,606 + Pf1N1B4_4713 0.41 +0.1 5,210,606 + Pf1N1B4_4713 0.41 -2.8 5,210,607 - Pf1N1B4_4713 0.41 -1.5 5,210,607 - Pf1N1B4_4713 0.41 -1.0 5,210,607 - -0.9 5,210,607 - Pf1N1B4_4713 0.41 -2.5 5,210,607 - Pf1N1B4_4713 0.41 -0.9 5,210,607 - Pf1N1B4_4713 0.41 +0.6 5,211,299 + Pf1N1B4_4713 0.87 -2.0 5,211,376 + +1.6 5,211,376 + -1.5 5,211,377 - -0.6 5,211,377 - -0.5 5,211,562 + -1.0 5,211,563 - +0.7 5,211,563 - -0.8 5,211,563 - -0.2 5,211,727 - -1.8 5,211,727 - +0.0 5,211,727 - -0.3 5,212,091 - Pf1N1B4_4715 0.40 -0.7 5,212,154 - Pf1N1B4_4715 0.48 +1.4 5,212,154 - Pf1N1B4_4715 0.48 +0.1
Or see this region's nucleotide sequence