Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4453

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4452 and Pf1N1B4_4453 are separated by 428 nucleotidesPf1N1B4_4453 and Pf1N1B4_4454 overlap by 4 nucleotidesPf1N1B4_4454 and Pf1N1B4_4455 are separated by 11 nucleotides Pf1N1B4_4452 - hypothetical protein, at 4,913,292 to 4,913,438 _4452 Pf1N1B4_4453 - Sensory histidine kinase QseC, at 4,913,867 to 4,915,270 _4453 Pf1N1B4_4454 - Two-component system response regulator QseB, at 4,915,267 to 4,915,929 _4454 Pf1N1B4_4455 - FIG00953528: hypothetical protein, at 4,915,941 to 4,916,585 _4455 Position (kb) 4913 4914 4915 4916Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4912.966 kb on + strandat 4913.123 kb on - strandat 4913.162 kb on + strandat 4913.162 kb on + strandat 4913.258 kb on + strandat 4913.284 kb on - strandat 4913.439 kb on + strandat 4913.440 kb on - strandat 4913.440 kb on - strandat 4913.500 kb on + strandat 4913.531 kb on + strandat 4913.531 kb on + strandat 4913.531 kb on + strandat 4913.559 kb on + strandat 4913.559 kb on + strandat 4913.560 kb on - strandat 4913.601 kb on + strandat 4913.691 kb on - strandat 4914.114 kb on + strand, within Pf1N1B4_4453at 4914.566 kb on + strand, within Pf1N1B4_4453at 4914.567 kb on - strand, within Pf1N1B4_4453at 4914.778 kb on + strand, within Pf1N1B4_4453at 4914.786 kb on - strand, within Pf1N1B4_4453at 4914.786 kb on - strand, within Pf1N1B4_4453at 4914.786 kb on - strand, within Pf1N1B4_4453at 4914.786 kb on - strand, within Pf1N1B4_4453at 4914.968 kb on + strand, within Pf1N1B4_4453at 4914.969 kb on - strand, within Pf1N1B4_4453at 4914.969 kb on - strand, within Pf1N1B4_4453at 4915.014 kb on + strand, within Pf1N1B4_4453at 4915.015 kb on - strand, within Pf1N1B4_4453at 4915.015 kb on - strand, within Pf1N1B4_4453at 4915.071 kb on + strand, within Pf1N1B4_4453at 4915.155 kb on + strandat 4915.156 kb on - strandat 4915.305 kb on + strandat 4915.351 kb on - strand, within Pf1N1B4_4454at 4915.502 kb on + strand, within Pf1N1B4_4454at 4915.502 kb on + strand, within Pf1N1B4_4454at 4915.919 kb on - strandat 4915.922 kb on - strandat 4916.107 kb on + strand, within Pf1N1B4_4455at 4916.108 kb on - strand, within Pf1N1B4_4455at 4916.212 kb on + strand, within Pf1N1B4_4455at 4916.212 kb on + strand, within Pf1N1B4_4455at 4916.231 kb on + strand, within Pf1N1B4_4455

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Per-strain Table

Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
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4,912,966 + -0.6
4,913,123 - -1.7
4,913,162 + +0.0
4,913,162 + -0.8
4,913,258 + -0.2
4,913,284 - +2.8
4,913,439 + -1.6
4,913,440 - -1.0
4,913,440 - +2.5
4,913,500 + -1.0
4,913,531 + -0.8
4,913,531 + -1.6
4,913,531 + +1.7
4,913,559 + -0.1
4,913,559 + -0.2
4,913,560 - -0.8
4,913,601 + +2.3
4,913,691 - +1.8
4,914,114 + Pf1N1B4_4453 0.18 -1.7
4,914,566 + Pf1N1B4_4453 0.50 -1.6
4,914,567 - Pf1N1B4_4453 0.50 -0.8
4,914,778 + Pf1N1B4_4453 0.65 +1.0
4,914,786 - Pf1N1B4_4453 0.65 +1.8
4,914,786 - Pf1N1B4_4453 0.65 -2.4
4,914,786 - Pf1N1B4_4453 0.65 +1.2
4,914,786 - Pf1N1B4_4453 0.65 -0.9
4,914,968 + Pf1N1B4_4453 0.78 -2.0
4,914,969 - Pf1N1B4_4453 0.78 -1.0
4,914,969 - Pf1N1B4_4453 0.78 -2.2
4,915,014 + Pf1N1B4_4453 0.82 -0.0
4,915,015 - Pf1N1B4_4453 0.82 -1.6
4,915,015 - Pf1N1B4_4453 0.82 +0.6
4,915,071 + Pf1N1B4_4453 0.86 +0.2
4,915,155 + +0.2
4,915,156 - +0.4
4,915,305 + +1.8
4,915,351 - Pf1N1B4_4454 0.13 -0.6
4,915,502 + Pf1N1B4_4454 0.35 +0.5
4,915,502 + Pf1N1B4_4454 0.35 -2.0
4,915,919 - +0.1
4,915,922 - -1.4
4,916,107 + Pf1N1B4_4455 0.26 -0.4
4,916,108 - Pf1N1B4_4455 0.26 +0.6
4,916,212 + Pf1N1B4_4455 0.42 -1.6
4,916,212 + Pf1N1B4_4455 0.42 -0.7
4,916,231 + Pf1N1B4_4455 0.45 -0.7

Or see this region's nucleotide sequence