Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4449 and Pf1N1B4_4450 are separated by 179 nucleotides Pf1N1B4_4450 and Pf1N1B4_4451 are separated by 133 nucleotides
Pf1N1B4_4449 - Permeases of the major facilitator superfamily, at 4,908,027 to 4,909,649
_4449
Pf1N1B4_4450 - Choline dehydrogenase (EC 1.1.99.1), at 4,909,829 to 4,911,478
_4450
Pf1N1B4_4451 - Transcriptional regulator, LysR family, at 4,911,612 to 4,912,496
_4451
Position (kb)
4909
4910
4911
4912 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4908.838 kb on + strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4909.097 kb on - strand, within Pf1N1B4_4449 at 4909.207 kb on + strand, within Pf1N1B4_4449 at 4909.207 kb on + strand at 4909.208 kb on - strand, within Pf1N1B4_4449 at 4909.304 kb on - strand, within Pf1N1B4_4449 at 4909.369 kb on + strand, within Pf1N1B4_4449 at 4909.528 kb on + strand at 4909.729 kb on - strand at 4909.729 kb on - strand at 4909.740 kb on + strand at 4909.740 kb on + strand at 4909.740 kb on + strand at 4909.741 kb on - strand at 4909.741 kb on - strand at 4909.754 kb on + strand at 4909.755 kb on - strand at 4909.830 kb on + strand at 4909.830 kb on + strand at 4909.830 kb on + strand at 4909.936 kb on + strand at 4909.937 kb on - strand at 4910.140 kb on - strand, within Pf1N1B4_4450 at 4910.213 kb on + strand, within Pf1N1B4_4450 at 4910.241 kb on + strand, within Pf1N1B4_4450 at 4910.241 kb on + strand, within Pf1N1B4_4450 at 4910.242 kb on - strand, within Pf1N1B4_4450 at 4910.242 kb on - strand at 4910.397 kb on + strand, within Pf1N1B4_4450 at 4910.488 kb on - strand, within Pf1N1B4_4450 at 4910.493 kb on + strand, within Pf1N1B4_4450 at 4910.804 kb on + strand, within Pf1N1B4_4450 at 4911.160 kb on - strand, within Pf1N1B4_4450 at 4911.160 kb on - strand, within Pf1N1B4_4450 at 4911.183 kb on + strand at 4911.401 kb on + strand at 4911.450 kb on + strand at 4911.451 kb on - strand at 4911.451 kb on - strand at 4912.104 kb on + strand, within Pf1N1B4_4451 at 4912.104 kb on + strand, within Pf1N1B4_4451 at 4912.104 kb on + strand, within Pf1N1B4_4451 at 4912.119 kb on + strand, within Pf1N1B4_4451 at 4912.120 kb on - strand at 4912.326 kb on + strand, within Pf1N1B4_4451 at 4912.326 kb on + strand, within Pf1N1B4_4451 at 4912.326 kb on + strand, within Pf1N1B4_4451 at 4912.327 kb on - strand, within Pf1N1B4_4451 at 4912.327 kb on - strand, within Pf1N1B4_4451 at 4912.400 kb on + strand, within Pf1N1B4_4451
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 4,908,838 + Pf1N1B4_4449 0.50 +0.1 4,908,839 - Pf1N1B4_4449 0.50 -2.2 4,908,839 - Pf1N1B4_4449 0.50 -1.4 4,908,839 - Pf1N1B4_4449 0.50 -1.6 4,909,097 - Pf1N1B4_4449 0.66 -0.2 4,909,207 + Pf1N1B4_4449 0.73 -0.4 4,909,207 + +0.8 4,909,208 - Pf1N1B4_4449 0.73 +0.8 4,909,304 - Pf1N1B4_4449 0.79 -1.6 4,909,369 + Pf1N1B4_4449 0.83 +0.2 4,909,528 + +0.3 4,909,729 - -1.1 4,909,729 - +0.3 4,909,740 + +0.9 4,909,740 + -1.1 4,909,740 + +0.5 4,909,741 - -1.8 4,909,741 - +0.4 4,909,754 + -0.2 4,909,755 - -1.4 4,909,830 + -1.9 4,909,830 + +0.2 4,909,830 + -2.0 4,909,936 + +0.5 4,909,937 - +0.5 4,910,140 - Pf1N1B4_4450 0.19 +0.8 4,910,213 + Pf1N1B4_4450 0.23 -0.4 4,910,241 + Pf1N1B4_4450 0.25 +0.0 4,910,241 + Pf1N1B4_4450 0.25 +0.4 4,910,242 - Pf1N1B4_4450 0.25 -1.0 4,910,242 - -1.2 4,910,397 + Pf1N1B4_4450 0.34 +0.3 4,910,488 - Pf1N1B4_4450 0.40 -0.2 4,910,493 + Pf1N1B4_4450 0.40 -1.6 4,910,804 + Pf1N1B4_4450 0.59 -2.6 4,911,160 - Pf1N1B4_4450 0.81 -1.6 4,911,160 - Pf1N1B4_4450 0.81 +0.5 4,911,183 + -0.8 4,911,401 + -2.5 4,911,450 + +0.2 4,911,451 - +0.9 4,911,451 - -0.6 4,912,104 + Pf1N1B4_4451 0.56 -1.2 4,912,104 + Pf1N1B4_4451 0.56 +0.3 4,912,104 + Pf1N1B4_4451 0.56 +0.1 4,912,119 + Pf1N1B4_4451 0.57 +0.2 4,912,120 - +0.3 4,912,326 + Pf1N1B4_4451 0.81 +0.6 4,912,326 + Pf1N1B4_4451 0.81 +1.4 4,912,326 + Pf1N1B4_4451 0.81 +1.0 4,912,327 - Pf1N1B4_4451 0.81 +2.2 4,912,327 - Pf1N1B4_4451 0.81 +1.3 4,912,400 + Pf1N1B4_4451 0.89 +0.2
Or see this region's nucleotide sequence