Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3412 and Pf1N1B4_3413 are separated by 78 nucleotides Pf1N1B4_3413 and Pf1N1B4_3414 are separated by 6 nucleotides Pf1N1B4_3414 and Pf1N1B4_3415 are separated by 141 nucleotides Pf1N1B4_3415 and Pf1N1B4_3416 are separated by 215 nucleotides
Pf1N1B4_3412 - CTP synthase (EC 6.3.4.2), at 3,802,988 to 3,803,695
_3412
Pf1N1B4_3413 - Transcriptional regulator, LysR family, at 3,803,774 to 3,804,652
_3413
Pf1N1B4_3414 - PROBABLE MEMBRANE PROTEIN, at 3,804,659 to 3,804,907
_3414
Pf1N1B4_3415 - Transcriptional regulator, TetR family, at 3,805,049 to 3,805,657
_3415
Pf1N1B4_3416 - Outer membrane protein romA, at 3,805,873 to 3,806,931
_3416
Position (kb)
3804
3805 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.808 kb on - strand at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.439 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand at 3804.817 kb on + strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.944 kb on + strand at 3804.945 kb on - strand at 3804.946 kb on + strand at 3804.947 kb on - strand at 3804.990 kb on + strand at 3804.991 kb on - strand at 3804.991 kb on - strand at 3804.995 kb on - strand at 3805.060 kb on - strand at 3805.060 kb on - strand at 3805.248 kb on - strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.528 kb on + strand, within Pf1N1B4_3415 at 3805.657 kb on + strand at 3805.657 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.704 kb on - strand at 3805.737 kb on - strand at 3805.737 kb on - strand at 3805.750 kb on + strand at 3805.761 kb on + strand at 3805.818 kb on + strand at 3805.819 kb on - strand at 3805.819 kb on - strand at 3805.884 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 3,803,734 + +0.4 3,803,734 + +1.0 3,803,734 + +0.2 3,803,734 + +1.0 3,803,734 + +0.3 3,803,735 - +4.0 3,803,735 - -0.1 3,803,735 - -0.8 3,803,735 - -0.5 3,803,735 - +0.2 3,803,735 - +0.8 3,803,735 - -1.5 3,803,735 - +1.4 3,803,808 - -0.2 3,804,308 + Pf1N1B4_3413 0.61 -0.4 3,804,308 + Pf1N1B4_3413 0.61 -1.4 3,804,439 + Pf1N1B4_3413 0.76 -2.1 3,804,465 + Pf1N1B4_3413 0.79 -2.0 3,804,465 + +1.1 3,804,817 + Pf1N1B4_3414 0.63 -1.5 3,804,818 - Pf1N1B4_3414 0.64 -1.5 3,804,818 - Pf1N1B4_3414 0.64 -0.8 3,804,818 - Pf1N1B4_3414 0.64 -1.9 3,804,944 + -1.1 3,804,945 - +1.8 3,804,946 + +1.4 3,804,947 - -0.5 3,804,990 + -0.4 3,804,991 - -1.0 3,804,991 - +0.0 3,804,995 - -2.7 3,805,060 - -2.0 3,805,060 - -1.2 3,805,248 - Pf1N1B4_3415 0.33 -0.7 3,805,515 + Pf1N1B4_3415 0.77 -0.5 3,805,515 + Pf1N1B4_3415 0.77 -2.1 3,805,515 + Pf1N1B4_3415 0.77 +0.8 3,805,516 - Pf1N1B4_3415 0.77 -1.3 3,805,516 - Pf1N1B4_3415 0.77 +0.1 3,805,516 - Pf1N1B4_3415 0.77 -1.2 3,805,528 + Pf1N1B4_3415 0.79 -0.8 3,805,657 + -0.5 3,805,657 + -1.4 3,805,661 + +0.2 3,805,661 + -1.4 3,805,661 + -1.0 3,805,704 - -0.2 3,805,737 - -0.2 3,805,737 - +0.2 3,805,750 + -2.4 3,805,761 + -1.1 3,805,818 + -0.8 3,805,819 - +0.8 3,805,819 - -0.5 3,805,884 - -1.1
Or see this region's nucleotide sequence