Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3123 and Pf1N1B4_3124 are separated by 67 nucleotides Pf1N1B4_3124 and Pf1N1B4_3125 overlap by 4 nucleotides Pf1N1B4_3125 and Pf1N1B4_3126 are separated by 127 nucleotides Pf1N1B4_3126 and Pf1N1B4_3127 overlap by 14 nucleotides
Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084
_3123
Pf1N1B4_3124 - Phage tail tube protein, at 3,510,152 to 3,510,499
_3124
Pf1N1B4_3125 - FIG00954242: hypothetical protein, at 3,510,496 to 3,510,792
_3125
Pf1N1B4_3126 - Phage tail length tape-measure protein, at 3,510,920 to 3,512,692
_3126
Pf1N1B4_3127 - Phage tail/DNA circulation protein, at 3,512,679 to 3,513,917
_3127
Position (kb)
3510
3511
3512
3513 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.016 kb on + strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.017 kb on - strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.067 kb on + strand at 3510.068 kb on - strand at 3510.132 kb on + strand at 3510.132 kb on + strand at 3510.133 kb on - strand at 3510.133 kb on - strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.179 kb on + strand at 3510.180 kb on - strand at 3510.209 kb on - strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.272 kb on + strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.273 kb on - strand, within Pf1N1B4_3124 at 3510.398 kb on + strand, within Pf1N1B4_3124 at 3510.416 kb on + strand, within Pf1N1B4_3124 at 3510.611 kb on - strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.664 kb on + strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.665 kb on - strand, within Pf1N1B4_3125 at 3510.805 kb on - strand at 3510.805 kb on - strand at 3510.805 kb on - strand at 3511.063 kb on + strand at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.422 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.756 kb on + strand, within Pf1N1B4_3126 at 3511.964 kb on - strand, within Pf1N1B4_3126 at 3511.964 kb on - strand, within Pf1N1B4_3126 at 3511.964 kb on - strand at 3511.964 kb on - strand, within Pf1N1B4_3126 at 3512.126 kb on + strand, within Pf1N1B4_3126 at 3513.198 kb on + strand, within Pf1N1B4_3127 at 3513.199 kb on - strand, within Pf1N1B4_3127 at 3513.199 kb on - strand, within Pf1N1B4_3127 at 3513.199 kb on - strand, within Pf1N1B4_3127 at 3513.324 kb on - strand, within Pf1N1B4_3127 at 3513.357 kb on + strand, within Pf1N1B4_3127 at 3513.690 kb on + strand, within Pf1N1B4_3127
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 3,510,016 + -0.8 3,510,016 + +0.2 3,510,016 + +2.5 3,510,017 - -0.7 3,510,017 - -1.5 3,510,017 - -1.4 3,510,017 - -1.8 3,510,017 - -1.0 3,510,067 + +0.8 3,510,067 + -1.1 3,510,067 + +0.4 3,510,067 + +0.7 3,510,068 - -1.0 3,510,132 + +0.2 3,510,132 + +0.2 3,510,133 - -1.7 3,510,133 - -1.2 3,510,179 + -0.0 3,510,179 + -1.9 3,510,179 + +1.3 3,510,180 - -1.9 3,510,209 - Pf1N1B4_3124 0.16 -0.7 3,510,272 + Pf1N1B4_3124 0.34 +2.8 3,510,272 + Pf1N1B4_3124 0.34 +1.2 3,510,272 + Pf1N1B4_3124 0.34 +1.4 3,510,273 - Pf1N1B4_3124 0.35 -2.1 3,510,273 - Pf1N1B4_3124 0.35 -1.2 3,510,273 - Pf1N1B4_3124 0.35 +1.4 3,510,273 - Pf1N1B4_3124 0.35 -0.8 3,510,273 - Pf1N1B4_3124 0.35 -0.8 3,510,273 - Pf1N1B4_3124 0.35 -1.0 3,510,273 - Pf1N1B4_3124 0.35 +0.6 3,510,398 + Pf1N1B4_3124 0.71 -1.0 3,510,416 + Pf1N1B4_3124 0.76 -1.9 3,510,611 - Pf1N1B4_3125 0.39 -0.1 3,510,664 + Pf1N1B4_3125 0.57 +0.8 3,510,664 + Pf1N1B4_3125 0.57 +0.6 3,510,664 + Pf1N1B4_3125 0.57 -0.4 3,510,664 + Pf1N1B4_3125 0.57 -0.5 3,510,664 + Pf1N1B4_3125 0.57 -1.8 3,510,664 + Pf1N1B4_3125 0.57 -1.2 3,510,665 - Pf1N1B4_3125 0.57 +1.7 3,510,665 - Pf1N1B4_3125 0.57 -0.5 3,510,665 - Pf1N1B4_3125 0.57 +2.4 3,510,805 - +0.5 3,510,805 - +1.8 3,510,805 - +0.5 3,511,063 + -0.5 3,511,422 + Pf1N1B4_3126 0.28 -2.4 3,511,422 + Pf1N1B4_3126 0.28 +1.2 3,511,422 + Pf1N1B4_3126 0.28 +0.4 3,511,756 + Pf1N1B4_3126 0.47 -1.0 3,511,756 + Pf1N1B4_3126 0.47 +0.2 3,511,756 + Pf1N1B4_3126 0.47 -0.0 3,511,964 - Pf1N1B4_3126 0.59 -0.9 3,511,964 - Pf1N1B4_3126 0.59 +1.3 3,511,964 - +1.8 3,511,964 - Pf1N1B4_3126 0.59 -0.2 3,512,126 + Pf1N1B4_3126 0.68 -1.0 3,513,198 + Pf1N1B4_3127 0.42 -0.5 3,513,199 - Pf1N1B4_3127 0.42 -0.7 3,513,199 - Pf1N1B4_3127 0.42 -1.8 3,513,199 - Pf1N1B4_3127 0.42 -1.0 3,513,324 - Pf1N1B4_3127 0.52 +0.3 3,513,357 + Pf1N1B4_3127 0.55 -0.5 3,513,690 + Pf1N1B4_3127 0.82 -1.0
Or see this region's nucleotide sequence