Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3124

Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3123 and Pf1N1B4_3124 are separated by 67 nucleotidesPf1N1B4_3124 and Pf1N1B4_3125 overlap by 4 nucleotidesPf1N1B4_3125 and Pf1N1B4_3126 are separated by 127 nucleotides Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084 _3123 Pf1N1B4_3124 - Phage tail tube protein, at 3,510,152 to 3,510,499 _3124 Pf1N1B4_3125 - FIG00954242: hypothetical protein, at 3,510,496 to 3,510,792 _3125 Pf1N1B4_3126 - Phage tail length tape-measure protein, at 3,510,920 to 3,512,692 _3126 Position (kb) 3510 3511Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3509.374 kb on + strand, within Pf1N1B4_3123at 3509.374 kb on + strand, within Pf1N1B4_3123at 3509.374 kb on + strand, within Pf1N1B4_3123at 3509.633 kb on - strand, within Pf1N1B4_3123at 3509.633 kb on - strand, within Pf1N1B4_3123at 3509.650 kb on + strand, within Pf1N1B4_3123at 3509.650 kb on + strand, within Pf1N1B4_3123at 3509.651 kb on - strand, within Pf1N1B4_3123at 3509.651 kb on - strand, within Pf1N1B4_3123at 3509.653 kb on + strand, within Pf1N1B4_3123at 3509.653 kb on + strand, within Pf1N1B4_3123at 3509.666 kb on - strand, within Pf1N1B4_3123at 3509.666 kb on - strand, within Pf1N1B4_3123at 3509.695 kb on + strand, within Pf1N1B4_3123at 3509.695 kb on + strand, within Pf1N1B4_3123at 3509.695 kb on + strand, within Pf1N1B4_3123at 3509.696 kb on - strand, within Pf1N1B4_3123at 3509.696 kb on - strand, within Pf1N1B4_3123at 3509.731 kb on + strand, within Pf1N1B4_3123at 3509.732 kb on - strand, within Pf1N1B4_3123at 3509.732 kb on - strand, within Pf1N1B4_3123at 3509.799 kb on + strand, within Pf1N1B4_3123at 3509.803 kb on + strand, within Pf1N1B4_3123at 3509.803 kb on + strand, within Pf1N1B4_3123at 3509.803 kb on + strand, within Pf1N1B4_3123at 3509.804 kb on - strand, within Pf1N1B4_3123at 3509.804 kb on - strand, within Pf1N1B4_3123at 3510.016 kb on + strandat 3510.016 kb on + strandat 3510.016 kb on + strandat 3510.017 kb on - strandat 3510.017 kb on - strandat 3510.017 kb on - strandat 3510.017 kb on - strandat 3510.017 kb on - strandat 3510.067 kb on + strandat 3510.067 kb on + strandat 3510.067 kb on + strandat 3510.067 kb on + strandat 3510.068 kb on - strandat 3510.132 kb on + strandat 3510.132 kb on + strandat 3510.133 kb on - strandat 3510.133 kb on - strandat 3510.179 kb on + strandat 3510.179 kb on + strandat 3510.179 kb on + strandat 3510.180 kb on - strandat 3510.209 kb on - strand, within Pf1N1B4_3124at 3510.272 kb on + strand, within Pf1N1B4_3124at 3510.272 kb on + strand, within Pf1N1B4_3124at 3510.272 kb on + strand, within Pf1N1B4_3124at 3510.273 kb on - strand, within Pf1N1B4_3124at 3510.273 kb on - strand, within Pf1N1B4_3124at 3510.273 kb on - strand, within Pf1N1B4_3124at 3510.273 kb on - strand, within Pf1N1B4_3124at 3510.273 kb on - strand, within Pf1N1B4_3124at 3510.273 kb on - strand, within Pf1N1B4_3124at 3510.273 kb on - strand, within Pf1N1B4_3124at 3510.398 kb on + strand, within Pf1N1B4_3124at 3510.416 kb on + strand, within Pf1N1B4_3124at 3510.611 kb on - strand, within Pf1N1B4_3125at 3510.664 kb on + strand, within Pf1N1B4_3125at 3510.664 kb on + strand, within Pf1N1B4_3125at 3510.664 kb on + strand, within Pf1N1B4_3125at 3510.664 kb on + strand, within Pf1N1B4_3125at 3510.664 kb on + strand, within Pf1N1B4_3125at 3510.664 kb on + strand, within Pf1N1B4_3125at 3510.665 kb on - strand, within Pf1N1B4_3125at 3510.665 kb on - strand, within Pf1N1B4_3125at 3510.665 kb on - strand, within Pf1N1B4_3125at 3510.805 kb on - strandat 3510.805 kb on - strandat 3510.805 kb on - strandat 3511.063 kb on + strandat 3511.422 kb on + strand, within Pf1N1B4_3126at 3511.422 kb on + strand, within Pf1N1B4_3126at 3511.422 kb on + strand, within Pf1N1B4_3126

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Per-strain Table

Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
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3,509,374 + Pf1N1B4_3123 0.53 +2.1
3,509,374 + Pf1N1B4_3123 0.53 +0.5
3,509,374 + Pf1N1B4_3123 0.53 +3.7
3,509,633 - Pf1N1B4_3123 0.70 -0.4
3,509,633 - Pf1N1B4_3123 0.70 +0.2
3,509,650 + Pf1N1B4_3123 0.71 +2.5
3,509,650 + Pf1N1B4_3123 0.71 +0.2
3,509,651 - Pf1N1B4_3123 0.71 +0.2
3,509,651 - Pf1N1B4_3123 0.71 -1.4
3,509,653 + Pf1N1B4_3123 0.71 -0.4
3,509,653 + Pf1N1B4_3123 0.71 +1.0
3,509,666 - Pf1N1B4_3123 0.72 +0.8
3,509,666 - Pf1N1B4_3123 0.72 +1.6
3,509,695 + Pf1N1B4_3123 0.74 +1.8
3,509,695 + Pf1N1B4_3123 0.74 -0.5
3,509,695 + Pf1N1B4_3123 0.74 +1.8
3,509,696 - Pf1N1B4_3123 0.74 -0.3
3,509,696 - Pf1N1B4_3123 0.74 -1.6
3,509,731 + Pf1N1B4_3123 0.76 -1.5
3,509,732 - Pf1N1B4_3123 0.76 -2.1
3,509,732 - Pf1N1B4_3123 0.76 -0.5
3,509,799 + Pf1N1B4_3123 0.81 -1.9
3,509,803 + Pf1N1B4_3123 0.81 +0.2
3,509,803 + Pf1N1B4_3123 0.81 -1.0
3,509,803 + Pf1N1B4_3123 0.81 -1.0
3,509,804 - Pf1N1B4_3123 0.81 -0.3
3,509,804 - Pf1N1B4_3123 0.81 -2.4
3,510,016 + -0.8
3,510,016 + +0.2
3,510,016 + +2.5
3,510,017 - -0.7
3,510,017 - -1.5
3,510,017 - -1.4
3,510,017 - -1.8
3,510,017 - -1.0
3,510,067 + +0.8
3,510,067 + -1.1
3,510,067 + +0.4
3,510,067 + +0.7
3,510,068 - -1.0
3,510,132 + +0.2
3,510,132 + +0.2
3,510,133 - -1.7
3,510,133 - -1.2
3,510,179 + -0.0
3,510,179 + -1.9
3,510,179 + +1.3
3,510,180 - -1.9
3,510,209 - Pf1N1B4_3124 0.16 -0.7
3,510,272 + Pf1N1B4_3124 0.34 +2.8
3,510,272 + Pf1N1B4_3124 0.34 +1.2
3,510,272 + Pf1N1B4_3124 0.34 +1.4
3,510,273 - Pf1N1B4_3124 0.35 -2.1
3,510,273 - Pf1N1B4_3124 0.35 -1.2
3,510,273 - Pf1N1B4_3124 0.35 +1.4
3,510,273 - Pf1N1B4_3124 0.35 -0.8
3,510,273 - Pf1N1B4_3124 0.35 -0.8
3,510,273 - Pf1N1B4_3124 0.35 -1.0
3,510,273 - Pf1N1B4_3124 0.35 +0.6
3,510,398 + Pf1N1B4_3124 0.71 -1.0
3,510,416 + Pf1N1B4_3124 0.76 -1.9
3,510,611 - Pf1N1B4_3125 0.39 -0.1
3,510,664 + Pf1N1B4_3125 0.57 +0.8
3,510,664 + Pf1N1B4_3125 0.57 +0.6
3,510,664 + Pf1N1B4_3125 0.57 -0.4
3,510,664 + Pf1N1B4_3125 0.57 -0.5
3,510,664 + Pf1N1B4_3125 0.57 -1.8
3,510,664 + Pf1N1B4_3125 0.57 -1.2
3,510,665 - Pf1N1B4_3125 0.57 +1.7
3,510,665 - Pf1N1B4_3125 0.57 -0.5
3,510,665 - Pf1N1B4_3125 0.57 +2.4
3,510,805 - +0.5
3,510,805 - +1.8
3,510,805 - +0.5
3,511,063 + -0.5
3,511,422 + Pf1N1B4_3126 0.28 -2.4
3,511,422 + Pf1N1B4_3126 0.28 +1.2
3,511,422 + Pf1N1B4_3126 0.28 +0.4

Or see this region's nucleotide sequence