Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_305 and Pf1N1B4_306 are separated by 217 nucleotides Pf1N1B4_306 and Pf1N1B4_307 are separated by 0 nucleotides Pf1N1B4_307 and Pf1N1B4_308 are separated by 247 nucleotides
Pf1N1B4_305 - Methyl-accepting chemotaxis protein, at 358,767 to 360,248
_305
Pf1N1B4_306 - Phage terminase, small subunit, at 360,466 to 360,834
_306
Pf1N1B4_307 - TesB-like acyl-CoA thioesterase 1, at 360,835 to 361,632
_307
Pf1N1B4_308 - FIG00956090: hypothetical protein, at 361,880 to 362,647
_308
Position (kb) 360
361
362 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 359.970 kb on + strand, within Pf1N1B4_305 at 359.970 kb on + strand, within Pf1N1B4_305 at 359.970 kb on + strand, within Pf1N1B4_305 at 359.971 kb on - strand, within Pf1N1B4_305 at 360.063 kb on - strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.229 kb on + strand at 360.230 kb on - strand at 360.230 kb on - strand at 360.291 kb on - strand at 360.291 kb on - strand at 360.309 kb on + strand at 360.309 kb on + strand at 360.309 kb on + strand at 360.310 kb on - strand at 360.370 kb on + strand at 360.370 kb on + strand at 360.370 kb on + strand at 360.371 kb on - strand at 360.376 kb on + strand at 360.376 kb on + strand at 360.459 kb on - strand at 360.519 kb on + strand, within Pf1N1B4_306 at 360.520 kb on - strand, within Pf1N1B4_306 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 361.043 kb on + strand, within Pf1N1B4_307 at 361.044 kb on - strand, within Pf1N1B4_307 at 361.086 kb on + strand, within Pf1N1B4_307 at 361.087 kb on - strand, within Pf1N1B4_307 at 361.237 kb on - strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.518 kb on - strand, within Pf1N1B4_307 at 361.578 kb on + strand at 361.578 kb on + strand at 361.579 kb on - strand at 361.579 kb on - strand at 361.699 kb on + strand at 361.700 kb on - strand at 362.115 kb on + strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 359,970 + Pf1N1B4_305 0.81 -1.5 359,970 + Pf1N1B4_305 0.81 +0.5 359,970 + Pf1N1B4_305 0.81 -0.4 359,971 - Pf1N1B4_305 0.81 +0.2 360,063 - +3.4 360,229 + -0.0 360,229 + -2.4 360,229 + +2.5 360,229 + -0.3 360,229 + -0.5 360,229 + -3.1 360,229 + -1.3 360,229 + +1.8 360,229 + -0.9 360,230 - -0.5 360,230 - +0.3 360,291 - +1.8 360,291 - +0.7 360,309 + -0.2 360,309 + -1.4 360,309 + -1.7 360,310 - -0.3 360,370 + -1.4 360,370 + -0.8 360,370 + -0.3 360,371 - -2.3 360,376 + +1.0 360,376 + +0.8 360,459 - -0.2 360,519 + Pf1N1B4_306 0.14 -0.4 360,520 - Pf1N1B4_306 0.15 -0.5 360,950 + Pf1N1B4_307 0.14 -1.4 360,950 + Pf1N1B4_307 0.14 -0.0 360,950 + Pf1N1B4_307 0.14 +2.5 360,950 + Pf1N1B4_307 0.14 -0.4 360,951 - Pf1N1B4_307 0.15 -1.5 360,951 - Pf1N1B4_307 0.15 +2.6 360,951 - Pf1N1B4_307 0.15 -0.5 360,951 - Pf1N1B4_307 0.15 -1.4 361,043 + Pf1N1B4_307 0.26 +0.9 361,044 - Pf1N1B4_307 0.26 -2.1 361,086 + Pf1N1B4_307 0.31 -1.0 361,087 - Pf1N1B4_307 0.32 +0.9 361,237 - Pf1N1B4_307 0.50 -0.1 361,238 + Pf1N1B4_307 0.51 +0.7 361,238 + Pf1N1B4_307 0.51 +0.1 361,238 + Pf1N1B4_307 0.51 +0.7 361,238 + Pf1N1B4_307 0.51 -0.2 361,239 - Pf1N1B4_307 0.51 -1.0 361,239 - Pf1N1B4_307 0.51 +0.6 361,239 - Pf1N1B4_307 0.51 -0.3 361,239 - Pf1N1B4_307 0.51 +2.0 361,239 - Pf1N1B4_307 0.51 -0.1 361,518 - Pf1N1B4_307 0.86 -1.0 361,578 + -0.0 361,578 + -0.0 361,579 - +1.2 361,579 - +1.4 361,699 + -1.5 361,700 - -1.2 362,115 + Pf1N1B4_308 0.31 -1.9 362,398 - Pf1N1B4_308 0.67 -0.1 362,398 - Pf1N1B4_308 0.67 -0.7 362,398 - Pf1N1B4_308 0.67 +0.1 362,534 + Pf1N1B4_308 0.85 -0.3 362,534 + Pf1N1B4_308 0.85 +1.0 362,535 - Pf1N1B4_308 0.85 -0.2 362,535 - Pf1N1B4_308 0.85 +1.3 362,535 - Pf1N1B4_308 0.85 +2.0 362,535 - Pf1N1B4_308 0.85 +0.2 362,535 - Pf1N1B4_308 0.85 +0.1 362,535 - Pf1N1B4_308 0.85 -1.9
Or see this region's nucleotide sequence