Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2720 and Pf1N1B4_2721 are separated by 185 nucleotides Pf1N1B4_2721 and Pf1N1B4_2722 are separated by 71 nucleotides Pf1N1B4_2722 and Pf1N1B4_2723 overlap by 6 nucleotides
Pf1N1B4_2720 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 3,058,059 to 3,059,708
_2720
Pf1N1B4_2721 - AMP nucleosidase (EC 3.2.2.4), at 3,059,894 to 3,061,357
_2721
Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064
_2722
Pf1N1B4_2723 - FIG00954142: hypothetical protein, at 3,062,059 to 3,062,268
_2723
Position (kb)
3059
3060
3061
3062 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.221 kb on - strand, within Pf1N1B4_2720 at 3059.221 kb on - strand, within Pf1N1B4_2720 at 3059.287 kb on - strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.566 kb on + strand at 3059.566 kb on + strand at 3059.566 kb on + strand at 3059.567 kb on - strand at 3059.640 kb on + strand at 3059.640 kb on + strand at 3059.640 kb on + strand at 3059.641 kb on - strand at 3059.733 kb on + strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.795 kb on + strand at 3059.796 kb on - strand at 3059.796 kb on - strand at 3059.908 kb on + strand at 3059.999 kb on + strand at 3060.000 kb on - strand at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.110 kb on + strand, within Pf1N1B4_2721 at 3060.111 kb on - strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.176 kb on + strand at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.177 kb on - strand, within Pf1N1B4_2721 at 3060.177 kb on - strand, within Pf1N1B4_2721 at 3060.263 kb on + strand, within Pf1N1B4_2721 at 3060.337 kb on + strand, within Pf1N1B4_2721 at 3060.380 kb on + strand, within Pf1N1B4_2721 at 3060.381 kb on - strand, within Pf1N1B4_2721 at 3060.464 kb on + strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.516 kb on - strand, within Pf1N1B4_2721 at 3060.689 kb on + strand at 3060.854 kb on + strand, within Pf1N1B4_2721 at 3060.854 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.235 kb on + strand at 3061.235 kb on + strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.390 kb on + strand at 3061.412 kb on + strand at 3061.413 kb on - strand at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.809 kb on + strand, within Pf1N1B4_2722 at 3062.060 kb on + strand at 3062.061 kb on - strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.063 kb on - strand at 3062.063 kb on - strand at 3062.131 kb on + strand, within Pf1N1B4_2723 at 3062.132 kb on - strand, within Pf1N1B4_2723 at 3062.294 kb on + strand at 3062.294 kb on + strand at 3062.295 kb on - strand at 3062.309 kb on + strand at 3062.309 kb on + strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.311 kb on + strand at 3062.311 kb on + strand at 3062.312 kb on - strand at 3062.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 3,059,220 + Pf1N1B4_2720 0.70 -0.3 3,059,220 + Pf1N1B4_2720 0.70 -1.4 3,059,220 + Pf1N1B4_2720 0.70 -0.3 3,059,221 - Pf1N1B4_2720 0.70 +0.1 3,059,221 - Pf1N1B4_2720 0.70 +0.3 3,059,287 - Pf1N1B4_2720 0.74 -1.5 3,059,377 + Pf1N1B4_2720 0.80 -0.5 3,059,377 + Pf1N1B4_2720 0.80 +2.2 3,059,377 + Pf1N1B4_2720 0.80 -0.7 3,059,377 + Pf1N1B4_2720 0.80 +0.1 3,059,378 - Pf1N1B4_2720 0.80 +0.9 3,059,378 - Pf1N1B4_2720 0.80 +0.9 3,059,378 - Pf1N1B4_2720 0.80 -0.1 3,059,566 + +0.9 3,059,566 + +0.1 3,059,566 + +1.1 3,059,567 - -0.1 3,059,640 + +2.9 3,059,640 + +0.4 3,059,640 + +0.7 3,059,641 - +0.3 3,059,733 + -0.7 3,059,734 - -1.5 3,059,734 - +1.3 3,059,734 - -0.3 3,059,734 - +0.7 3,059,795 + -0.0 3,059,796 - -0.7 3,059,796 - +1.0 3,059,908 + -0.1 3,059,999 + -1.3 3,060,000 - +2.3 3,060,072 - Pf1N1B4_2721 0.12 +0.3 3,060,072 - Pf1N1B4_2721 0.12 -0.5 3,060,072 - Pf1N1B4_2721 0.12 +1.9 3,060,072 - Pf1N1B4_2721 0.12 +0.1 3,060,072 - Pf1N1B4_2721 0.12 -0.7 3,060,072 - Pf1N1B4_2721 0.12 -1.5 3,060,110 + Pf1N1B4_2721 0.15 -0.9 3,060,111 - Pf1N1B4_2721 0.15 +0.4 3,060,137 + Pf1N1B4_2721 0.17 -0.8 3,060,137 + Pf1N1B4_2721 0.17 +0.3 3,060,137 + Pf1N1B4_2721 0.17 -0.3 3,060,137 + Pf1N1B4_2721 0.17 -0.6 3,060,138 - Pf1N1B4_2721 0.17 -0.3 3,060,138 - Pf1N1B4_2721 0.17 +0.2 3,060,138 - Pf1N1B4_2721 0.17 -1.7 3,060,138 - Pf1N1B4_2721 0.17 -0.7 3,060,176 + -0.9 3,060,176 + Pf1N1B4_2721 0.19 +0.5 3,060,176 + Pf1N1B4_2721 0.19 +0.3 3,060,176 + Pf1N1B4_2721 0.19 -1.3 3,060,177 - Pf1N1B4_2721 0.19 -1.0 3,060,177 - Pf1N1B4_2721 0.19 -2.6 3,060,263 + Pf1N1B4_2721 0.25 -0.4 3,060,337 + Pf1N1B4_2721 0.30 +0.1 3,060,380 + Pf1N1B4_2721 0.33 +1.9 3,060,381 - Pf1N1B4_2721 0.33 -1.0 3,060,464 + Pf1N1B4_2721 0.39 -0.1 3,060,465 - Pf1N1B4_2721 0.39 +1.3 3,060,465 - Pf1N1B4_2721 0.39 +1.5 3,060,465 - Pf1N1B4_2721 0.39 +0.3 3,060,515 + Pf1N1B4_2721 0.42 +2.3 3,060,515 + Pf1N1B4_2721 0.42 +0.3 3,060,515 + Pf1N1B4_2721 0.42 -2.9 3,060,516 - Pf1N1B4_2721 0.42 -0.5 3,060,689 + +1.9 3,060,854 + Pf1N1B4_2721 0.66 +1.3 3,060,854 + Pf1N1B4_2721 0.66 +1.1 3,060,872 + Pf1N1B4_2721 0.67 +0.4 3,060,872 + Pf1N1B4_2721 0.67 -0.7 3,060,872 + Pf1N1B4_2721 0.67 -0.9 3,060,872 + Pf1N1B4_2721 0.67 +0.3 3,060,872 + Pf1N1B4_2721 0.67 +0.5 3,060,872 + Pf1N1B4_2721 0.67 -0.1 3,060,872 + Pf1N1B4_2721 0.67 +0.1 3,060,873 - Pf1N1B4_2721 0.67 -0.1 3,060,873 - Pf1N1B4_2721 0.67 +1.6 3,060,873 - Pf1N1B4_2721 0.67 +0.8 3,060,873 - Pf1N1B4_2721 0.67 +0.3 3,061,034 + Pf1N1B4_2721 0.78 -1.3 3,061,034 + Pf1N1B4_2721 0.78 +1.2 3,061,034 + Pf1N1B4_2721 0.78 +1.1 3,061,034 + Pf1N1B4_2721 0.78 -1.3 3,061,035 - Pf1N1B4_2721 0.78 +0.7 3,061,035 - Pf1N1B4_2721 0.78 -1.7 3,061,035 - Pf1N1B4_2721 0.78 -0.1 3,061,235 + -2.2 3,061,235 + -0.1 3,061,236 - +0.3 3,061,236 - +0.1 3,061,236 - +0.5 3,061,236 - +1.9 3,061,236 - +2.1 3,061,390 + +0.8 3,061,412 + -0.5 3,061,413 - -1.9 3,061,732 + Pf1N1B4_2722 0.48 +1.9 3,061,732 + Pf1N1B4_2722 0.48 -0.9 3,061,732 + Pf1N1B4_2722 0.48 -0.8 3,061,732 + Pf1N1B4_2722 0.48 +0.8 3,061,733 - Pf1N1B4_2722 0.48 +2.3 3,061,733 - Pf1N1B4_2722 0.48 +0.4 3,061,733 - Pf1N1B4_2722 0.48 -1.8 3,061,733 - Pf1N1B4_2722 0.48 -0.5 3,061,733 - Pf1N1B4_2722 0.48 +1.6 3,061,733 - Pf1N1B4_2722 0.48 -1.7 3,061,809 + Pf1N1B4_2722 0.60 -1.9 3,062,060 + -1.1 3,062,061 - +1.9 3,062,062 + +1.0 3,062,062 + -0.9 3,062,062 + +2.2 3,062,062 + +0.5 3,062,062 + -0.9 3,062,062 + -1.3 3,062,063 - +0.1 3,062,063 - -1.8 3,062,131 + Pf1N1B4_2723 0.34 -0.0 3,062,132 - Pf1N1B4_2723 0.35 +0.8 3,062,294 + +0.6 3,062,294 + +0.5 3,062,295 - -1.5 3,062,309 + +0.9 3,062,309 + +0.6 3,062,310 - -0.7 3,062,310 - +0.3 3,062,310 - -0.0 3,062,311 + -0.7 3,062,311 + -0.1 3,062,312 - +1.9 3,062,312 - +0.3
Or see this region's nucleotide sequence