Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_233 and Pf1N1B4_234 are separated by 307 nucleotides Pf1N1B4_234 and Pf1N1B4_235 are separated by 65 nucleotides Pf1N1B4_235 and Pf1N1B4_236 are separated by 22 nucleotides Pf1N1B4_236 and Pf1N1B4_237 are separated by 81 nucleotides
Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536
_233
Pf1N1B4_234 - hypothetical protein, at 294,844 to 295,152
_234
Pf1N1B4_235 - Molybdopterin biosynthesis protein MoeA, at 295,218 to 296,405
_235
Pf1N1B4_236 - Molybdenum cofactor biosynthesis protein MoaB, at 296,428 to 296,967
_236
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Position (kb) 295
296
297 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 294.264 kb on + strand, within Pf1N1B4_233 at 294.265 kb on - strand at 294.351 kb on - strand, within Pf1N1B4_233 at 294.555 kb on + strand at 294.555 kb on + strand at 294.555 kb on + strand at 294.556 kb on - strand at 294.556 kb on - strand at 294.578 kb on + strand at 294.579 kb on - strand at 294.579 kb on - strand at 294.600 kb on + strand at 294.601 kb on - strand at 294.628 kb on + strand at 294.649 kb on - strand at 294.649 kb on - strand at 294.694 kb on + strand at 294.695 kb on - strand at 294.695 kb on - strand at 294.730 kb on + strand at 294.734 kb on + strand at 294.735 kb on - strand at 294.735 kb on - strand at 294.821 kb on + strand at 294.997 kb on + strand at 294.997 kb on + strand, within Pf1N1B4_234 at 294.997 kb on + strand, within Pf1N1B4_234 at 294.997 kb on + strand, within Pf1N1B4_234 at 294.998 kb on - strand, within Pf1N1B4_234 at 294.998 kb on - strand, within Pf1N1B4_234 at 295.150 kb on + strand at 295.150 kb on + strand at 295.150 kb on + strand at 295.216 kb on + strand at 295.217 kb on - strand at 295.275 kb on - strand at 295.348 kb on + strand, within Pf1N1B4_235 at 295.349 kb on - strand, within Pf1N1B4_235 at 295.387 kb on + strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.388 kb on - strand, within Pf1N1B4_235 at 295.483 kb on + strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.777 kb on - strand, within Pf1N1B4_235 at 295.812 kb on - strand, within Pf1N1B4_235 at 295.819 kb on + strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.949 kb on + strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.240 kb on - strand, within Pf1N1B4_235 at 296.638 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.838 kb on + strand, within Pf1N1B4_236 at 296.884 kb on - strand, within Pf1N1B4_236 at 296.947 kb on + strand at 296.948 kb on - strand at 296.948 kb on - strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.968 kb on - strand at 296.968 kb on - strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.984 kb on - strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.004 kb on - strand at 297.004 kb on - strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.006 kb on - strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 294,264 + Pf1N1B4_233 0.71 -0.3 294,265 - -1.4 294,351 - Pf1N1B4_233 0.80 +1.1 294,555 + +0.2 294,555 + -0.9 294,555 + -0.2 294,556 - -0.8 294,556 - -0.2 294,578 + +0.2 294,579 - -0.8 294,579 - +1.2 294,600 + +0.6 294,601 - -1.1 294,628 + -1.3 294,649 - -1.3 294,649 - +0.2 294,694 + -1.5 294,695 - +0.1 294,695 - -0.2 294,730 + -0.5 294,734 + -0.7 294,735 - -0.4 294,735 - -0.5 294,821 + +3.1 294,997 + -0.4 294,997 + Pf1N1B4_234 0.50 -0.8 294,997 + Pf1N1B4_234 0.50 +4.7 294,997 + Pf1N1B4_234 0.50 -1.2 294,998 - Pf1N1B4_234 0.50 +0.2 294,998 - Pf1N1B4_234 0.50 +0.8 295,150 + +0.9 295,150 + -1.1 295,150 + -0.8 295,216 + -0.2 295,217 - -0.4 295,275 - -1.0 295,348 + Pf1N1B4_235 0.11 -1.8 295,349 - Pf1N1B4_235 0.11 +0.8 295,387 + Pf1N1B4_235 0.14 -2.2 295,388 - Pf1N1B4_235 0.14 +0.2 295,388 - Pf1N1B4_235 0.14 -0.5 295,388 - Pf1N1B4_235 0.14 -1.0 295,483 + Pf1N1B4_235 0.22 -0.4 295,735 - Pf1N1B4_235 0.44 -1.2 295,735 - Pf1N1B4_235 0.44 -1.5 295,735 - Pf1N1B4_235 0.44 -2.3 295,777 - Pf1N1B4_235 0.47 -1.0 295,812 - Pf1N1B4_235 0.50 -0.2 295,819 + Pf1N1B4_235 0.51 -2.2 295,820 - Pf1N1B4_235 0.51 -0.8 295,820 - Pf1N1B4_235 0.51 +0.2 295,820 - Pf1N1B4_235 0.51 -1.2 295,820 - Pf1N1B4_235 0.51 -0.8 295,820 - Pf1N1B4_235 0.51 -1.3 295,949 + Pf1N1B4_235 0.62 -0.3 296,053 - Pf1N1B4_235 0.70 -1.0 296,053 - Pf1N1B4_235 0.70 +0.8 296,148 - Pf1N1B4_235 0.78 -0.7 296,148 - Pf1N1B4_235 0.78 -0.7 296,240 - Pf1N1B4_235 0.86 +0.1 296,638 + Pf1N1B4_236 0.39 -1.2 296,821 + Pf1N1B4_236 0.73 +0.6 296,821 + Pf1N1B4_236 0.73 +0.5 296,821 + Pf1N1B4_236 0.73 -1.2 296,838 + Pf1N1B4_236 0.76 -1.0 296,884 - Pf1N1B4_236 0.84 +0.3 296,947 + -0.7 296,948 - -0.4 296,948 - -0.5 296,967 + +0.3 296,967 + +1.7 296,967 + -1.4 296,968 - -1.9 296,968 - -0.7 296,983 + -1.8 296,983 + -0.6 296,983 + -2.2 296,984 - +1.2 297,003 + -1.3 297,003 + -1.0 297,003 + -0.8 297,003 + -1.5 297,003 + -2.2 297,003 + +0.8 297,003 + -0.7 297,003 + -0.9 297,003 + -1.0 297,004 - +0.2 297,004 - +0.9 297,005 + +0.5 297,005 + -2.2 297,005 + -0.9 297,006 - +0.9 297,045 + +3.0 297,045 + -0.5 297,045 + -0.5 297,045 + -1.4 297,045 + -0.4 297,046 - +0.2 297,046 - -0.8 297,060 + +0.1 297,060 + -0.5 297,061 - +0.4 297,061 - -1.5 297,061 - -1.1 297,238 + Pf1N1B4_237 0.31 +1.1 297,239 - Pf1N1B4_237 0.32 -0.5 297,239 - Pf1N1B4_237 0.32 -0.4 297,239 - Pf1N1B4_237 0.32 -1.7 297,341 + Pf1N1B4_237 0.48 -0.7 297,341 + Pf1N1B4_237 0.48 +0.1 297,341 + Pf1N1B4_237 0.48 -1.0 297,342 - Pf1N1B4_237 0.49 -1.5
Or see this region's nucleotide sequence