Experiment: Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_195 and Pf1N1B4_196 are separated by 335 nucleotides Pf1N1B4_196 and Pf1N1B4_197 overlap by 4 nucleotides Pf1N1B4_197 and Pf1N1B4_198 overlap by 4 nucleotides Pf1N1B4_198 and Pf1N1B4_199 overlap by 4 nucleotides
Pf1N1B4_195 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 243,777 to 244,235
_195
Pf1N1B4_196 - FIG137594: Putative iron-regulated membrane protein, at 244,571 to 245,776
_196
Pf1N1B4_197 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 245,773 to 246,315
_197
Pf1N1B4_198 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 246,312 to 246,632
_198
Pf1N1B4_199 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 246,629 to 247,204
_199
Position (kb) 244
245
246 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 243.617 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.719 kb on + strand at 243.719 kb on + strand at 243.784 kb on + strand at 243.804 kb on + strand at 243.822 kb on + strand at 243.826 kb on + strand, within Pf1N1B4_195 at 243.826 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.939 kb on - strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.055 kb on + strand, within Pf1N1B4_195 at 244.111 kb on - strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195 at 244.223 kb on + strand at 244.224 kb on - strand at 244.224 kb on - strand at 244.260 kb on + strand at 244.261 kb on - strand at 244.295 kb on + strand at 244.295 kb on + strand at 244.400 kb on + strand at 244.418 kb on - strand at 244.453 kb on + strand at 244.454 kb on - strand at 244.464 kb on + strand at 244.567 kb on - strand at 244.612 kb on + strand at 244.613 kb on - strand at 244.644 kb on + strand at 244.757 kb on + strand, within Pf1N1B4_196 at 244.758 kb on - strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.890 kb on - strand at 244.890 kb on - strand, within Pf1N1B4_196 at 244.890 kb on - strand, within Pf1N1B4_196 at 245.255 kb on - strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.483 kb on + strand, within Pf1N1B4_196 at 245.483 kb on + strand, within Pf1N1B4_196 at 245.484 kb on - strand, within Pf1N1B4_196 at 245.887 kb on + strand, within Pf1N1B4_197 at 245.888 kb on - strand, within Pf1N1B4_197 at 245.888 kb on - strand, within Pf1N1B4_197 at 246.025 kb on + strand, within Pf1N1B4_197 at 246.025 kb on + strand at 246.337 kb on + strand at 246.338 kb on - strand at 246.338 kb on - strand at 246.338 kb on - strand at 246.341 kb on - strand at 246.564 kb on + strand, within Pf1N1B4_198 at 246.564 kb on + strand, within Pf1N1B4_198 at 246.564 kb on + strand at 246.564 kb on + strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.634 kb on + strand at 246.634 kb on + strand at 246.635 kb on - strand at 246.635 kb on - strand at 246.635 kb on - strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199
Per-strain Table
Position Strand Gene LocusTag Fraction Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 remove 243,617 + -2.5 243,713 + +0.5 243,713 + -0.6 243,713 + -1.9 243,713 + -0.5 243,713 + -1.7 243,713 + +0.7 243,713 + -1.1 243,715 + +1.2 243,715 + +0.5 243,715 + -0.5 243,715 + -1.1 243,715 + +3.4 243,719 + -1.0 243,719 + +0.1 243,784 + +1.5 243,804 + -0.2 243,822 + -0.4 243,826 + Pf1N1B4_195 0.11 -1.2 243,826 + Pf1N1B4_195 0.11 +1.8 243,938 + Pf1N1B4_195 0.35 +0.6 243,938 + Pf1N1B4_195 0.35 +0.2 243,939 - Pf1N1B4_195 0.35 -0.6 244,029 + Pf1N1B4_195 0.55 +1.5 244,029 + Pf1N1B4_195 0.55 -0.8 244,029 + Pf1N1B4_195 0.55 -0.2 244,029 + Pf1N1B4_195 0.55 +1.1 244,030 - Pf1N1B4_195 0.55 +1.2 244,030 - Pf1N1B4_195 0.55 +2.3 244,030 - Pf1N1B4_195 0.55 +1.6 244,030 - Pf1N1B4_195 0.55 -1.4 244,055 + Pf1N1B4_195 0.61 -1.7 244,111 - Pf1N1B4_195 0.73 -0.9 244,153 + Pf1N1B4_195 0.82 -0.8 244,153 + +0.4 244,153 + Pf1N1B4_195 0.82 +2.0 244,153 + +1.4 244,153 + Pf1N1B4_195 0.82 -0.4 244,153 + Pf1N1B4_195 0.82 +0.9 244,153 + Pf1N1B4_195 0.82 +2.3 244,154 - Pf1N1B4_195 0.82 -2.0 244,154 - Pf1N1B4_195 0.82 -2.0 244,223 + +0.6 244,224 - +1.6 244,224 - +0.3 244,260 + +2.1 244,261 - -0.8 244,295 + -1.1 244,295 + -1.2 244,400 + +0.5 244,418 - +0.1 244,453 + -1.3 244,454 - -0.2 244,464 + +0.6 244,567 - -0.4 244,612 + -0.1 244,613 - +2.1 244,644 + +0.8 244,757 + Pf1N1B4_196 0.15 -0.9 244,758 - Pf1N1B4_196 0.16 -0.7 244,889 + Pf1N1B4_196 0.26 -0.8 244,889 + Pf1N1B4_196 0.26 -1.4 244,889 + Pf1N1B4_196 0.26 +0.6 244,890 - -0.2 244,890 - Pf1N1B4_196 0.26 -0.9 244,890 - Pf1N1B4_196 0.26 -0.8 245,255 - Pf1N1B4_196 0.57 -1.0 245,414 + Pf1N1B4_196 0.70 -1.8 245,414 + Pf1N1B4_196 0.70 -0.4 245,414 + Pf1N1B4_196 0.70 -3.2 245,483 + Pf1N1B4_196 0.76 -1.6 245,483 + Pf1N1B4_196 0.76 -1.8 245,484 - Pf1N1B4_196 0.76 -2.7 245,887 + Pf1N1B4_197 0.21 -0.5 245,888 - Pf1N1B4_197 0.21 -2.1 245,888 - Pf1N1B4_197 0.21 -2.3 246,025 + Pf1N1B4_197 0.46 -1.6 246,025 + -1.0 246,337 + +0.8 246,338 - -1.0 246,338 - -2.3 246,338 - -0.3 246,341 - -0.5 246,564 + Pf1N1B4_198 0.79 -0.6 246,564 + Pf1N1B4_198 0.79 +0.1 246,564 + -0.8 246,564 + Pf1N1B4_198 0.79 +1.2 246,565 - Pf1N1B4_198 0.79 +0.1 246,565 - Pf1N1B4_198 0.79 +0.2 246,565 - Pf1N1B4_198 0.79 +0.5 246,565 - Pf1N1B4_198 0.79 -2.6 246,634 + -1.7 246,634 + +0.3 246,635 - +1.0 246,635 - +2.1 246,635 - -1.0 246,686 + -0.3 246,686 + -1.2 246,686 + +0.8 246,686 + -0.5 246,686 + -0.2 246,686 + +0.9 246,686 + +0.0 246,687 - Pf1N1B4_199 0.10 -0.0 246,687 - Pf1N1B4_199 0.10 +0.7 246,687 - Pf1N1B4_199 0.10 -1.3 246,687 - Pf1N1B4_199 0.10 +2.4 246,687 - Pf1N1B4_199 0.10 -1.5 246,687 - Pf1N1B4_199 0.10 -1.5 246,687 - Pf1N1B4_199 0.10 +1.8
Or see this region's nucleotide sequence