Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_98 and Pf1N1B4_99 are separated by 24 nucleotides Pf1N1B4_99 and Pf1N1B4_100 are separated by 308 nucleotides Pf1N1B4_100 and Pf1N1B4_101 are separated by 64 nucleotides
Pf1N1B4_98 - Nucleoside-specific channel-forming protein Tsx precursor, at 103,531 to 104,487
_98
Pf1N1B4_99 - Purine nucleoside permease, at 104,512 to 105,546
_99
Pf1N1B4_100 - FIG00953437: hypothetical protein, at 105,855 to 106,082
_100
Pf1N1B4_101 - hypothetical protein, at 106,147 to 106,311
_101
Position (kb)
104
105
106 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 103.529 kb on + strand at 103.705 kb on + strand, within Pf1N1B4_98 at 103.789 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.799 kb on - strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand at 103.834 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand at 103.993 kb on + strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98 at 104.014 kb on + strand, within Pf1N1B4_98 at 104.014 kb on + strand, within Pf1N1B4_98 at 104.015 kb on - strand, within Pf1N1B4_98 at 104.015 kb on - strand, within Pf1N1B4_98 at 104.056 kb on + strand, within Pf1N1B4_98 at 104.056 kb on + strand at 104.056 kb on + strand, within Pf1N1B4_98 at 104.057 kb on - strand, within Pf1N1B4_98 at 104.116 kb on + strand, within Pf1N1B4_98 at 104.159 kb on - strand, within Pf1N1B4_98 at 104.191 kb on + strand, within Pf1N1B4_98 at 104.191 kb on + strand, within Pf1N1B4_98 at 104.191 kb on + strand at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.237 kb on - strand, within Pf1N1B4_98 at 104.332 kb on + strand, within Pf1N1B4_98 at 104.461 kb on + strand at 104.485 kb on + strand at 104.485 kb on + strand at 104.512 kb on - strand at 104.715 kb on + strand, within Pf1N1B4_99 at 104.830 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.903 kb on - strand, within Pf1N1B4_99 at 104.903 kb on - strand at 105.020 kb on - strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.255 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.269 kb on - strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.302 kb on - strand at 105.302 kb on - strand, within Pf1N1B4_99 at 105.428 kb on - strand at 105.657 kb on + strand at 105.657 kb on + strand at 105.657 kb on + strand at 105.658 kb on - strand at 105.791 kb on - strand at 105.828 kb on - strand at 105.828 kb on - strand at 105.829 kb on + strand at 105.830 kb on - strand at 105.830 kb on - strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.845 kb on + strand at 105.846 kb on - strand at 105.854 kb on + strand at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.886 kb on - strand, within Pf1N1B4_100 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.227 kb on - strand, within Pf1N1B4_101 at 106.293 kb on + strand, within Pf1N1B4_101 at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.428 kb on - strand at 106.428 kb on - strand at 106.501 kb on + strand at 106.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 103,529 + +0.3 103,705 + Pf1N1B4_98 0.18 -2.1 103,789 + Pf1N1B4_98 0.27 -0.1 103,798 + Pf1N1B4_98 0.28 -1.2 103,798 + Pf1N1B4_98 0.28 -1.0 103,799 - Pf1N1B4_98 0.28 +1.1 103,804 + Pf1N1B4_98 0.29 +1.0 103,804 + Pf1N1B4_98 0.29 +1.7 103,804 + Pf1N1B4_98 0.29 +0.9 103,805 - Pf1N1B4_98 0.29 -1.2 103,805 - Pf1N1B4_98 0.29 -1.1 103,805 - Pf1N1B4_98 0.29 +0.0 103,805 - Pf1N1B4_98 0.29 +0.6 103,805 - -0.7 103,834 + Pf1N1B4_98 0.32 +0.4 103,993 + Pf1N1B4_98 0.48 +2.2 103,993 + Pf1N1B4_98 0.48 +1.6 103,993 + -0.4 103,993 + Pf1N1B4_98 0.48 +0.6 103,994 - Pf1N1B4_98 0.48 -0.7 103,994 - Pf1N1B4_98 0.48 -0.6 104,014 + Pf1N1B4_98 0.50 -0.6 104,014 + Pf1N1B4_98 0.50 -1.9 104,015 - Pf1N1B4_98 0.51 +0.5 104,015 - Pf1N1B4_98 0.51 +0.4 104,056 + Pf1N1B4_98 0.55 -0.7 104,056 + +1.6 104,056 + Pf1N1B4_98 0.55 +0.4 104,057 - Pf1N1B4_98 0.55 +0.8 104,116 + Pf1N1B4_98 0.61 +0.6 104,159 - Pf1N1B4_98 0.66 +1.3 104,191 + Pf1N1B4_98 0.69 +1.3 104,191 + Pf1N1B4_98 0.69 +0.1 104,191 + -0.7 104,192 - Pf1N1B4_98 0.69 +1.6 104,192 - Pf1N1B4_98 0.69 +0.5 104,192 - Pf1N1B4_98 0.69 -1.7 104,192 - Pf1N1B4_98 0.69 +1.6 104,192 - Pf1N1B4_98 0.69 -1.7 104,236 + Pf1N1B4_98 0.74 +1.1 104,236 + Pf1N1B4_98 0.74 +0.0 104,236 + Pf1N1B4_98 0.74 -0.9 104,236 + Pf1N1B4_98 0.74 -0.9 104,237 - Pf1N1B4_98 0.74 -1.5 104,332 + Pf1N1B4_98 0.84 -0.4 104,461 + -0.4 104,485 + +1.4 104,485 + -1.1 104,512 - -0.7 104,715 + Pf1N1B4_99 0.20 -1.8 104,830 + Pf1N1B4_99 0.31 -0.5 104,902 + Pf1N1B4_99 0.38 -0.1 104,902 + Pf1N1B4_99 0.38 -0.5 104,903 - Pf1N1B4_99 0.38 -2.0 104,903 - +1.2 105,020 - Pf1N1B4_99 0.49 +0.0 105,192 + Pf1N1B4_99 0.66 -1.8 105,192 + Pf1N1B4_99 0.66 -0.9 105,255 + Pf1N1B4_99 0.72 -0.1 105,268 + Pf1N1B4_99 0.73 -0.1 105,268 + Pf1N1B4_99 0.73 -0.7 105,269 - Pf1N1B4_99 0.73 -1.1 105,301 + Pf1N1B4_99 0.76 -0.7 105,301 + Pf1N1B4_99 0.76 +2.1 105,301 + Pf1N1B4_99 0.76 -1.6 105,301 + Pf1N1B4_99 0.76 -1.2 105,301 + Pf1N1B4_99 0.76 -0.7 105,302 - -0.7 105,302 - Pf1N1B4_99 0.76 +0.3 105,428 - +0.0 105,657 + +1.3 105,657 + +1.4 105,657 + -1.1 105,658 - +0.2 105,791 - -0.3 105,828 - -0.3 105,828 - -0.2 105,829 + -1.3 105,830 - -0.3 105,830 - +1.2 105,833 + +0.0 105,833 + -1.0 105,833 + +0.0 105,834 - +1.2 105,834 - +0.9 105,834 - +0.2 105,834 - +0.6 105,834 - +0.3 105,834 - -0.2 105,834 - -0.5 105,834 - +0.5 105,845 + +2.0 105,846 - -0.1 105,854 + +0.5 105,885 + Pf1N1B4_100 0.13 +1.7 105,885 + Pf1N1B4_100 0.13 -0.3 105,885 + Pf1N1B4_100 0.13 -0.6 105,886 - Pf1N1B4_100 0.14 -0.4 106,226 + Pf1N1B4_101 0.48 +1.3 106,226 + Pf1N1B4_101 0.48 -0.4 106,227 - Pf1N1B4_101 0.48 -1.6 106,293 + Pf1N1B4_101 0.88 -1.9 106,424 + +0.6 106,424 + -2.0 106,424 + +0.1 106,424 + -0.5 106,428 - +0.7 106,428 - +0.4 106,501 + -0.4 106,502 - +0.9
Or see this region's nucleotide sequence