Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_840 and Pf1N1B4_841 are separated by 231 nucleotides Pf1N1B4_841 and Pf1N1B4_842 are separated by 184 nucleotides Pf1N1B4_842 and Pf1N1B4_843 are separated by 100 nucleotides
Pf1N1B4_840 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 967,990 to 969,459
_840
Pf1N1B4_841 - ABC-type sugar transport systems, ATPase components, at 969,691 to 970,239
_841
Pf1N1B4_842 - putative membrane protein, at 970,424 to 971,191
_842
Pf1N1B4_843 - Transcriptional regulator, LysR family, at 971,292 to 972,242
_843
Position (kb)
970
971
972 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 969.528 kb on - strand at 969.621 kb on + strand at 969.668 kb on + strand at 969.668 kb on + strand at 969.673 kb on + strand at 969.674 kb on - strand at 969.692 kb on + strand at 969.693 kb on - strand at 969.693 kb on - strand at 969.783 kb on + strand, within Pf1N1B4_841 at 969.784 kb on - strand, within Pf1N1B4_841 at 969.784 kb on - strand at 969.885 kb on + strand, within Pf1N1B4_841 at 969.989 kb on + strand, within Pf1N1B4_841 at 969.990 kb on - strand, within Pf1N1B4_841 at 969.990 kb on - strand, within Pf1N1B4_841 at 969.990 kb on - strand, within Pf1N1B4_841 at 969.990 kb on - strand, within Pf1N1B4_841 at 970.193 kb on - strand at 970.251 kb on - strand at 970.329 kb on + strand at 970.329 kb on + strand at 970.330 kb on - strand at 970.330 kb on - strand at 970.405 kb on - strand at 970.405 kb on - strand at 970.437 kb on + strand at 970.438 kb on - strand at 970.438 kb on - strand at 970.599 kb on + strand, within Pf1N1B4_842 at 970.599 kb on + strand at 970.600 kb on - strand, within Pf1N1B4_842 at 970.704 kb on + strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.705 kb on - strand, within Pf1N1B4_842 at 970.713 kb on + strand, within Pf1N1B4_842 at 970.714 kb on - strand, within Pf1N1B4_842 at 970.715 kb on + strand, within Pf1N1B4_842 at 970.716 kb on - strand, within Pf1N1B4_842 at 970.811 kb on - strand, within Pf1N1B4_842 at 970.811 kb on - strand, within Pf1N1B4_842 at 970.824 kb on + strand, within Pf1N1B4_842 at 971.102 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand, within Pf1N1B4_842 at 971.109 kb on + strand at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.110 kb on - strand, within Pf1N1B4_842 at 971.174 kb on + strand at 971.175 kb on - strand at 971.175 kb on - strand at 971.208 kb on + strand at 971.209 kb on - strand at 971.248 kb on + strand at 971.251 kb on + strand at 971.251 kb on + strand at 971.252 kb on - strand at 971.367 kb on + strand at 971.372 kb on + strand at 971.540 kb on + strand, within Pf1N1B4_843 at 971.541 kb on - strand, within Pf1N1B4_843 at 971.600 kb on + strand, within Pf1N1B4_843 at 971.600 kb on + strand, within Pf1N1B4_843 at 971.601 kb on - strand, within Pf1N1B4_843 at 971.601 kb on - strand, within Pf1N1B4_843 at 971.634 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.816 kb on + strand, within Pf1N1B4_843 at 971.816 kb on + strand at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.923 kb on - strand, within Pf1N1B4_843 at 972.161 kb on + strand at 972.161 kb on + strand at 972.161 kb on + strand at 972.161 kb on + strand at 972.162 kb on - strand at 972.162 kb on - strand at 972.167 kb on + strand at 972.167 kb on + strand at 972.168 kb on - strand at 972.168 kb on - strand at 972.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 969,528 - -0.7 969,621 + -0.9 969,668 + +0.1 969,668 + -1.2 969,673 + +0.3 969,674 - +0.8 969,692 + +0.5 969,693 - -0.7 969,693 - +0.4 969,783 + Pf1N1B4_841 0.17 -1.8 969,784 - Pf1N1B4_841 0.17 -0.5 969,784 - -0.0 969,885 + Pf1N1B4_841 0.35 +1.5 969,989 + Pf1N1B4_841 0.54 -0.8 969,990 - Pf1N1B4_841 0.54 +0.0 969,990 - Pf1N1B4_841 0.54 -0.2 969,990 - Pf1N1B4_841 0.54 -0.4 969,990 - Pf1N1B4_841 0.54 -2.0 970,193 - -0.0 970,251 - -0.0 970,329 + +0.1 970,329 + -0.6 970,330 - +0.9 970,330 - -1.6 970,405 - +0.2 970,405 - -1.3 970,437 + +1.0 970,438 - -1.6 970,438 - +0.5 970,599 + Pf1N1B4_842 0.23 +0.5 970,599 + +2.1 970,600 - Pf1N1B4_842 0.23 +3.3 970,704 + Pf1N1B4_842 0.36 +0.5 970,705 - Pf1N1B4_842 0.37 +0.9 970,705 - Pf1N1B4_842 0.37 +0.6 970,705 - Pf1N1B4_842 0.37 -1.0 970,705 - Pf1N1B4_842 0.37 -0.0 970,705 - Pf1N1B4_842 0.37 -0.5 970,713 + Pf1N1B4_842 0.38 -0.5 970,714 - Pf1N1B4_842 0.38 +1.5 970,715 + Pf1N1B4_842 0.38 +0.1 970,716 - Pf1N1B4_842 0.38 -0.0 970,811 - Pf1N1B4_842 0.50 -1.9 970,811 - Pf1N1B4_842 0.50 +1.1 970,824 + Pf1N1B4_842 0.52 +0.9 971,102 + Pf1N1B4_842 0.88 +0.4 971,109 + Pf1N1B4_842 0.89 +2.3 971,109 + Pf1N1B4_842 0.89 +1.6 971,109 + Pf1N1B4_842 0.89 +1.1 971,109 + +1.1 971,110 - Pf1N1B4_842 0.89 -0.7 971,110 - Pf1N1B4_842 0.89 -1.5 971,110 - Pf1N1B4_842 0.89 -0.9 971,110 - Pf1N1B4_842 0.89 -0.5 971,110 - Pf1N1B4_842 0.89 -0.0 971,174 + -1.3 971,175 - -0.3 971,175 - -0.7 971,208 + -0.4 971,209 - -0.0 971,248 + +1.0 971,251 + +1.1 971,251 + +0.5 971,252 - +0.8 971,367 + +3.5 971,372 + +1.4 971,540 + Pf1N1B4_843 0.26 +1.0 971,541 - Pf1N1B4_843 0.26 +0.6 971,600 + Pf1N1B4_843 0.32 -1.3 971,600 + Pf1N1B4_843 0.32 -0.6 971,601 - Pf1N1B4_843 0.32 -2.5 971,601 - Pf1N1B4_843 0.32 +1.1 971,634 + Pf1N1B4_843 0.36 +0.2 971,784 + Pf1N1B4_843 0.52 -1.0 971,784 + Pf1N1B4_843 0.52 +0.4 971,784 + Pf1N1B4_843 0.52 -1.3 971,816 + Pf1N1B4_843 0.55 +1.5 971,816 + -0.5 971,922 + Pf1N1B4_843 0.66 +0.5 971,922 + Pf1N1B4_843 0.66 -1.5 971,922 + Pf1N1B4_843 0.66 +0.2 971,922 + Pf1N1B4_843 0.66 +1.1 971,923 - Pf1N1B4_843 0.66 +0.5 972,161 + -1.3 972,161 + +0.1 972,161 + -0.2 972,161 + -1.5 972,162 - -0.3 972,162 - -0.0 972,167 + +0.9 972,167 + +0.5 972,168 - +0.1 972,168 - +0.7 972,168 - -2.4
Or see this region's nucleotide sequence