Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_842

Experiment: Nitrate reduction, replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_840 and Pf1N1B4_841 are separated by 231 nucleotidesPf1N1B4_841 and Pf1N1B4_842 are separated by 184 nucleotidesPf1N1B4_842 and Pf1N1B4_843 are separated by 100 nucleotides Pf1N1B4_840 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 967,990 to 969,459 _840 Pf1N1B4_841 - ABC-type sugar transport systems, ATPase components, at 969,691 to 970,239 _841 Pf1N1B4_842 - putative membrane protein, at 970,424 to 971,191 _842 Pf1N1B4_843 - Transcriptional regulator, LysR family, at 971,292 to 972,242 _843 Position (kb) 970 971 972Strain fitness (log2 ratio) -2 -1 0 1 2 3at 969.528 kb on - strandat 969.621 kb on + strandat 969.668 kb on + strandat 969.668 kb on + strandat 969.673 kb on + strandat 969.674 kb on - strandat 969.692 kb on + strandat 969.693 kb on - strandat 969.693 kb on - strandat 969.783 kb on + strand, within Pf1N1B4_841at 969.784 kb on - strand, within Pf1N1B4_841at 969.784 kb on - strandat 969.885 kb on + strand, within Pf1N1B4_841at 969.989 kb on + strand, within Pf1N1B4_841at 969.990 kb on - strand, within Pf1N1B4_841at 969.990 kb on - strand, within Pf1N1B4_841at 969.990 kb on - strand, within Pf1N1B4_841at 969.990 kb on - strand, within Pf1N1B4_841at 970.193 kb on - strandat 970.251 kb on - strandat 970.329 kb on + strandat 970.329 kb on + strandat 970.330 kb on - strandat 970.330 kb on - strandat 970.405 kb on - strandat 970.405 kb on - strandat 970.437 kb on + strandat 970.438 kb on - strandat 970.438 kb on - strandat 970.599 kb on + strand, within Pf1N1B4_842at 970.599 kb on + strandat 970.600 kb on - strand, within Pf1N1B4_842at 970.704 kb on + strand, within Pf1N1B4_842at 970.705 kb on - strand, within Pf1N1B4_842at 970.705 kb on - strand, within Pf1N1B4_842at 970.705 kb on - strand, within Pf1N1B4_842at 970.705 kb on - strand, within Pf1N1B4_842at 970.705 kb on - strand, within Pf1N1B4_842at 970.713 kb on + strand, within Pf1N1B4_842at 970.714 kb on - strand, within Pf1N1B4_842at 970.715 kb on + strand, within Pf1N1B4_842at 970.716 kb on - strand, within Pf1N1B4_842at 970.811 kb on - strand, within Pf1N1B4_842at 970.811 kb on - strand, within Pf1N1B4_842at 970.824 kb on + strand, within Pf1N1B4_842at 971.102 kb on + strand, within Pf1N1B4_842at 971.109 kb on + strand, within Pf1N1B4_842at 971.109 kb on + strand, within Pf1N1B4_842at 971.109 kb on + strand, within Pf1N1B4_842at 971.109 kb on + strandat 971.110 kb on - strand, within Pf1N1B4_842at 971.110 kb on - strand, within Pf1N1B4_842at 971.110 kb on - strand, within Pf1N1B4_842at 971.110 kb on - strand, within Pf1N1B4_842at 971.110 kb on - strand, within Pf1N1B4_842at 971.174 kb on + strandat 971.175 kb on - strandat 971.175 kb on - strandat 971.208 kb on + strandat 971.209 kb on - strandat 971.248 kb on + strandat 971.251 kb on + strandat 971.251 kb on + strandat 971.252 kb on - strandat 971.367 kb on + strandat 971.372 kb on + strandat 971.540 kb on + strand, within Pf1N1B4_843at 971.541 kb on - strand, within Pf1N1B4_843at 971.600 kb on + strand, within Pf1N1B4_843at 971.600 kb on + strand, within Pf1N1B4_843at 971.601 kb on - strand, within Pf1N1B4_843at 971.601 kb on - strand, within Pf1N1B4_843at 971.634 kb on + strand, within Pf1N1B4_843at 971.784 kb on + strand, within Pf1N1B4_843at 971.784 kb on + strand, within Pf1N1B4_843at 971.784 kb on + strand, within Pf1N1B4_843at 971.816 kb on + strand, within Pf1N1B4_843at 971.816 kb on + strandat 971.922 kb on + strand, within Pf1N1B4_843at 971.922 kb on + strand, within Pf1N1B4_843at 971.922 kb on + strand, within Pf1N1B4_843at 971.922 kb on + strand, within Pf1N1B4_843at 971.923 kb on - strand, within Pf1N1B4_843at 972.161 kb on + strandat 972.161 kb on + strandat 972.161 kb on + strandat 972.161 kb on + strandat 972.162 kb on - strandat 972.162 kb on - strandat 972.167 kb on + strandat 972.167 kb on + strandat 972.168 kb on - strandat 972.168 kb on - strandat 972.168 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C
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969,528 - -0.7
969,621 + -0.9
969,668 + +0.1
969,668 + -1.2
969,673 + +0.3
969,674 - +0.8
969,692 + +0.5
969,693 - -0.7
969,693 - +0.4
969,783 + Pf1N1B4_841 0.17 -1.8
969,784 - Pf1N1B4_841 0.17 -0.5
969,784 - -0.0
969,885 + Pf1N1B4_841 0.35 +1.5
969,989 + Pf1N1B4_841 0.54 -0.8
969,990 - Pf1N1B4_841 0.54 +0.0
969,990 - Pf1N1B4_841 0.54 -0.2
969,990 - Pf1N1B4_841 0.54 -0.4
969,990 - Pf1N1B4_841 0.54 -2.0
970,193 - -0.0
970,251 - -0.0
970,329 + +0.1
970,329 + -0.6
970,330 - +0.9
970,330 - -1.6
970,405 - +0.2
970,405 - -1.3
970,437 + +1.0
970,438 - -1.6
970,438 - +0.5
970,599 + Pf1N1B4_842 0.23 +0.5
970,599 + +2.1
970,600 - Pf1N1B4_842 0.23 +3.3
970,704 + Pf1N1B4_842 0.36 +0.5
970,705 - Pf1N1B4_842 0.37 +0.9
970,705 - Pf1N1B4_842 0.37 +0.6
970,705 - Pf1N1B4_842 0.37 -1.0
970,705 - Pf1N1B4_842 0.37 -0.0
970,705 - Pf1N1B4_842 0.37 -0.5
970,713 + Pf1N1B4_842 0.38 -0.5
970,714 - Pf1N1B4_842 0.38 +1.5
970,715 + Pf1N1B4_842 0.38 +0.1
970,716 - Pf1N1B4_842 0.38 -0.0
970,811 - Pf1N1B4_842 0.50 -1.9
970,811 - Pf1N1B4_842 0.50 +1.1
970,824 + Pf1N1B4_842 0.52 +0.9
971,102 + Pf1N1B4_842 0.88 +0.4
971,109 + Pf1N1B4_842 0.89 +2.3
971,109 + Pf1N1B4_842 0.89 +1.6
971,109 + Pf1N1B4_842 0.89 +1.1
971,109 + +1.1
971,110 - Pf1N1B4_842 0.89 -0.7
971,110 - Pf1N1B4_842 0.89 -1.5
971,110 - Pf1N1B4_842 0.89 -0.9
971,110 - Pf1N1B4_842 0.89 -0.5
971,110 - Pf1N1B4_842 0.89 -0.0
971,174 + -1.3
971,175 - -0.3
971,175 - -0.7
971,208 + -0.4
971,209 - -0.0
971,248 + +1.0
971,251 + +1.1
971,251 + +0.5
971,252 - +0.8
971,367 + +3.5
971,372 + +1.4
971,540 + Pf1N1B4_843 0.26 +1.0
971,541 - Pf1N1B4_843 0.26 +0.6
971,600 + Pf1N1B4_843 0.32 -1.3
971,600 + Pf1N1B4_843 0.32 -0.6
971,601 - Pf1N1B4_843 0.32 -2.5
971,601 - Pf1N1B4_843 0.32 +1.1
971,634 + Pf1N1B4_843 0.36 +0.2
971,784 + Pf1N1B4_843 0.52 -1.0
971,784 + Pf1N1B4_843 0.52 +0.4
971,784 + Pf1N1B4_843 0.52 -1.3
971,816 + Pf1N1B4_843 0.55 +1.5
971,816 + -0.5
971,922 + Pf1N1B4_843 0.66 +0.5
971,922 + Pf1N1B4_843 0.66 -1.5
971,922 + Pf1N1B4_843 0.66 +0.2
971,922 + Pf1N1B4_843 0.66 +1.1
971,923 - Pf1N1B4_843 0.66 +0.5
972,161 + -1.3
972,161 + +0.1
972,161 + -0.2
972,161 + -1.5
972,162 - -0.3
972,162 - -0.0
972,167 + +0.9
972,167 + +0.5
972,168 - +0.1
972,168 - +0.7
972,168 - -2.4

Or see this region's nucleotide sequence