Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_815

Experiment: Nitrate reduction, replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_814 and Pf1N1B4_815 overlap by 4 nucleotidesPf1N1B4_815 and Pf1N1B4_816 are separated by 161 nucleotidesPf1N1B4_816 and Pf1N1B4_817 are separated by 41 nucleotides Pf1N1B4_814 - Transcriptional regulator, PadR family, at 932,291 to 932,872 _814 Pf1N1B4_815 - iron-chelator utilization protein, at 932,869 to 933,666 _815 Pf1N1B4_816 - Integral membrane protein, at 933,828 to 934,433 _816 Pf1N1B4_817 - FIG00955104: hypothetical protein, at 934,475 to 934,705 _817 Position (kb) 932 933 934Strain fitness (log2 ratio) -2 -1 0 1 2at 932.108 kb on + strandat 932.146 kb on + strandat 932.148 kb on + strandat 932.148 kb on + strandat 932.148 kb on + strandat 932.251 kb on + strandat 932.267 kb on + strandat 932.460 kb on - strand, within Pf1N1B4_814at 932.502 kb on - strand, within Pf1N1B4_814at 932.525 kb on + strand, within Pf1N1B4_814at 932.626 kb on - strand, within Pf1N1B4_814at 932.809 kb on + strand, within Pf1N1B4_814at 932.810 kb on - strand, within Pf1N1B4_814at 932.989 kb on - strand, within Pf1N1B4_815at 932.989 kb on - strand, within Pf1N1B4_815at 932.989 kb on - strand, within Pf1N1B4_815at 933.044 kb on + strand, within Pf1N1B4_815at 933.044 kb on + strand, within Pf1N1B4_815at 933.044 kb on + strand, within Pf1N1B4_815at 933.045 kb on - strand, within Pf1N1B4_815at 933.045 kb on - strand, within Pf1N1B4_815at 933.045 kb on - strand, within Pf1N1B4_815at 933.045 kb on - strand, within Pf1N1B4_815at 933.045 kb on - strand, within Pf1N1B4_815at 933.145 kb on + strand, within Pf1N1B4_815at 933.146 kb on - strand, within Pf1N1B4_815at 933.146 kb on - strand, within Pf1N1B4_815at 933.160 kb on + strand, within Pf1N1B4_815at 933.310 kb on + strand, within Pf1N1B4_815at 933.310 kb on + strand, within Pf1N1B4_815at 933.311 kb on - strand, within Pf1N1B4_815at 933.311 kb on - strandat 933.311 kb on - strandat 933.311 kb on - strand, within Pf1N1B4_815at 933.535 kb on + strand, within Pf1N1B4_815at 933.535 kb on + strand, within Pf1N1B4_815at 933.536 kb on - strand, within Pf1N1B4_815at 933.584 kb on + strand, within Pf1N1B4_815at 933.664 kb on + strandat 933.694 kb on - strandat 933.958 kb on + strand, within Pf1N1B4_816at 934.006 kb on + strand, within Pf1N1B4_816at 934.007 kb on - strand, within Pf1N1B4_816at 934.007 kb on - strand, within Pf1N1B4_816at 934.007 kb on - strandat 934.007 kb on - strand, within Pf1N1B4_816at 934.018 kb on + strand, within Pf1N1B4_816at 934.019 kb on - strand, within Pf1N1B4_816at 934.019 kb on - strand, within Pf1N1B4_816at 934.019 kb on - strand, within Pf1N1B4_816at 934.055 kb on + strand, within Pf1N1B4_816at 934.056 kb on - strand, within Pf1N1B4_816at 934.082 kb on - strand, within Pf1N1B4_816at 934.177 kb on + strand, within Pf1N1B4_816at 934.178 kb on - strand, within Pf1N1B4_816at 934.375 kb on - strandat 934.375 kb on - strandat 934.375 kb on - strandat 934.375 kb on - strandat 934.379 kb on - strandat 934.379 kb on - strandat 934.379 kb on - strandat 934.390 kb on + strandat 934.390 kb on + strandat 934.391 kb on - strandat 934.391 kb on - strandat 934.509 kb on + strand, within Pf1N1B4_817at 934.510 kb on - strand, within Pf1N1B4_817at 934.573 kb on + strand, within Pf1N1B4_817at 934.574 kb on - strandat 934.632 kb on + strand, within Pf1N1B4_817at 934.632 kb on + strand, within Pf1N1B4_817at 934.632 kb on + strand, within Pf1N1B4_817at 934.633 kb on - strand, within Pf1N1B4_817

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C
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932,108 + -0.5
932,146 + -1.6
932,148 + -0.6
932,148 + +0.2
932,148 + +0.1
932,251 + -0.0
932,267 + -2.1
932,460 - Pf1N1B4_814 0.29 +0.5
932,502 - Pf1N1B4_814 0.36 +0.7
932,525 + Pf1N1B4_814 0.40 +0.1
932,626 - Pf1N1B4_814 0.58 -0.7
932,809 + Pf1N1B4_814 0.89 +0.7
932,810 - Pf1N1B4_814 0.89 +1.3
932,989 - Pf1N1B4_815 0.15 +0.5
932,989 - Pf1N1B4_815 0.15 +0.7
932,989 - Pf1N1B4_815 0.15 +1.0
933,044 + Pf1N1B4_815 0.22 -0.6
933,044 + Pf1N1B4_815 0.22 +0.1
933,044 + Pf1N1B4_815 0.22 -1.0
933,045 - Pf1N1B4_815 0.22 +1.0
933,045 - Pf1N1B4_815 0.22 +0.9
933,045 - Pf1N1B4_815 0.22 +0.7
933,045 - Pf1N1B4_815 0.22 -2.0
933,045 - Pf1N1B4_815 0.22 +1.5
933,145 + Pf1N1B4_815 0.35 +0.9
933,146 - Pf1N1B4_815 0.35 +1.3
933,146 - Pf1N1B4_815 0.35 +0.8
933,160 + Pf1N1B4_815 0.36 -1.8
933,310 + Pf1N1B4_815 0.55 +0.7
933,310 + Pf1N1B4_815 0.55 +2.3
933,311 - Pf1N1B4_815 0.55 -1.5
933,311 - -0.2
933,311 - -2.2
933,311 - Pf1N1B4_815 0.55 -0.3
933,535 + Pf1N1B4_815 0.83 +0.2
933,535 + Pf1N1B4_815 0.83 -0.7
933,536 - Pf1N1B4_815 0.84 +1.2
933,584 + Pf1N1B4_815 0.90 -0.3
933,664 + +0.3
933,694 - +0.6
933,958 + Pf1N1B4_816 0.21 +1.0
934,006 + Pf1N1B4_816 0.29 +2.0
934,007 - Pf1N1B4_816 0.30 +0.9
934,007 - Pf1N1B4_816 0.30 -1.8
934,007 - +0.8
934,007 - Pf1N1B4_816 0.30 -0.0
934,018 + Pf1N1B4_816 0.31 +0.3
934,019 - Pf1N1B4_816 0.32 -0.7
934,019 - Pf1N1B4_816 0.32 -0.8
934,019 - Pf1N1B4_816 0.32 +0.3
934,055 + Pf1N1B4_816 0.37 -1.1
934,056 - Pf1N1B4_816 0.38 -0.6
934,082 - Pf1N1B4_816 0.42 -0.2
934,177 + Pf1N1B4_816 0.58 -0.8
934,178 - Pf1N1B4_816 0.58 +0.1
934,375 - +0.2
934,375 - -0.3
934,375 - -0.1
934,375 - +1.8
934,379 - -1.0
934,379 - +0.5
934,379 - +1.5
934,390 + -1.0
934,390 + -0.1
934,391 - +0.2
934,391 - +0.5
934,509 + Pf1N1B4_817 0.15 -0.5
934,510 - Pf1N1B4_817 0.15 -0.4
934,573 + Pf1N1B4_817 0.42 +0.8
934,574 - +1.5
934,632 + Pf1N1B4_817 0.68 -1.1
934,632 + Pf1N1B4_817 0.68 -1.3
934,632 + Pf1N1B4_817 0.68 -0.5
934,633 - Pf1N1B4_817 0.68 -0.9

Or see this region's nucleotide sequence