Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_6

Experiment: Nitrate reduction, replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4 and Pf1N1B4_5 are separated by 183 nucleotidesPf1N1B4_5 and Pf1N1B4_6 are separated by 41 nucleotidesPf1N1B4_6 and Pf1N1B4_7 are separated by 63 nucleotides Pf1N1B4_4 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 3,678 to 4,598 _4 Pf1N1B4_5 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 4,782 to 4,922 _5 Pf1N1B4_6 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 4,964 to 5,401 _6 Pf1N1B4_7 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 5,465 to 6,319 _7 Position (kb) 4 5 6Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4.673 kb on - strandat 4.673 kb on - strandat 4.673 kb on - strandat 4.673 kb on - strandat 4.673 kb on - strandat 4.673 kb on - strandat 4.724 kb on - strandat 4.724 kb on - strandat 4.724 kb on - strandat 4.736 kb on - strandat 4.736 kb on - strandat 4.852 kb on - strand, within Pf1N1B4_5at 5.014 kb on - strand, within Pf1N1B4_6at 5.014 kb on - strand, within Pf1N1B4_6at 5.014 kb on - strand, within Pf1N1B4_6at 5.014 kb on - strand, within Pf1N1B4_6at 5.014 kb on - strand, within Pf1N1B4_6at 5.014 kb on - strand, within Pf1N1B4_6at 5.014 kb on - strand, within Pf1N1B4_6at 5.014 kb on - strand, within Pf1N1B4_6at 5.103 kb on + strand, within Pf1N1B4_6at 5.104 kb on - strandat 5.104 kb on - strand, within Pf1N1B4_6at 5.104 kb on - strand, within Pf1N1B4_6at 5.104 kb on - strand, within Pf1N1B4_6at 5.104 kb on - strand, within Pf1N1B4_6at 5.304 kb on + strand, within Pf1N1B4_6at 5.305 kb on - strand, within Pf1N1B4_6at 5.361 kb on - strandat 5.361 kb on - strandat 5.361 kb on - strandat 5.512 kb on - strandat 5.512 kb on - strandat 5.552 kb on + strand, within Pf1N1B4_7at 5.560 kb on - strand, within Pf1N1B4_7at 5.603 kb on - strand, within Pf1N1B4_7at 5.753 kb on + strandat 5.753 kb on + strand, within Pf1N1B4_7at 5.754 kb on - strand, within Pf1N1B4_7at 5.754 kb on - strand, within Pf1N1B4_7at 5.754 kb on - strand, within Pf1N1B4_7at 5.887 kb on + strand, within Pf1N1B4_7at 5.888 kb on - strand, within Pf1N1B4_7at 5.928 kb on + strand, within Pf1N1B4_7at 5.929 kb on - strand, within Pf1N1B4_7at 5.929 kb on - strand, within Pf1N1B4_7at 6.247 kb on - strandat 6.305 kb on - strandat 6.305 kb on - strandat 6.308 kb on + strandat 6.308 kb on + strandat 6.308 kb on + strandat 6.309 kb on - strandat 6.309 kb on - strandat 6.309 kb on - strandat 6.309 kb on - strandat 6.310 kb on + strandat 6.311 kb on - strandat 6.357 kb on + strandat 6.357 kb on + strandat 6.357 kb on + strandat 6.358 kb on - strandat 6.358 kb on - strandat 6.358 kb on - strandat 6.358 kb on - strandat 6.359 kb on + strandat 6.359 kb on + strandat 6.359 kb on + strandat 6.359 kb on + strandat 6.359 kb on + strandat 6.360 kb on - strandat 6.360 kb on - strandat 6.360 kb on - strandat 6.360 kb on - strandat 6.360 kb on - strandat 6.360 kb on - strandat 6.360 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C
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4,673 - -1.2
4,673 - -1.7
4,673 - -0.4
4,673 - -0.3
4,673 - +0.8
4,673 - -0.3
4,724 - +0.1
4,724 - -0.2
4,724 - -0.1
4,736 - +2.5
4,736 - +0.4
4,852 - Pf1N1B4_5 0.50 -2.8
5,014 - Pf1N1B4_6 0.11 -3.9
5,014 - Pf1N1B4_6 0.11 -4.5
5,014 - Pf1N1B4_6 0.11 -2.2
5,014 - Pf1N1B4_6 0.11 -3.5
5,014 - Pf1N1B4_6 0.11 -2.2
5,014 - Pf1N1B4_6 0.11 -0.7
5,014 - Pf1N1B4_6 0.11 -1.0
5,014 - Pf1N1B4_6 0.11 -2.3
5,103 + Pf1N1B4_6 0.32 -2.2
5,104 - -1.0
5,104 - Pf1N1B4_6 0.32 +0.6
5,104 - Pf1N1B4_6 0.32 -1.6
5,104 - Pf1N1B4_6 0.32 -2.1
5,104 - Pf1N1B4_6 0.32 -2.9
5,304 + Pf1N1B4_6 0.78 -3.8
5,305 - Pf1N1B4_6 0.78 -2.8
5,361 - -1.5
5,361 - -1.1
5,361 - -2.7
5,512 - -0.9
5,512 - +0.8
5,552 + Pf1N1B4_7 0.10 +3.6
5,560 - Pf1N1B4_7 0.11 -0.2
5,603 - Pf1N1B4_7 0.16 -0.7
5,753 + +1.6
5,753 + Pf1N1B4_7 0.34 +0.6
5,754 - Pf1N1B4_7 0.34 -1.2
5,754 - Pf1N1B4_7 0.34 +0.1
5,754 - Pf1N1B4_7 0.34 +0.2
5,887 + Pf1N1B4_7 0.49 +0.4
5,888 - Pf1N1B4_7 0.49 -0.4
5,928 + Pf1N1B4_7 0.54 -1.3
5,929 - Pf1N1B4_7 0.54 +0.2
5,929 - Pf1N1B4_7 0.54 +0.0
6,247 - -2.1
6,305 - +0.0
6,305 - -0.4
6,308 + -0.7
6,308 + +1.4
6,308 + -1.2
6,309 - -0.4
6,309 - -1.0
6,309 - +0.7
6,309 - -0.9
6,310 + +0.0
6,311 - -0.1
6,357 + -2.0
6,357 + +0.2
6,357 + +0.9
6,358 - +0.8
6,358 - -1.9
6,358 - -0.2
6,358 - +2.2
6,359 + -0.4
6,359 + -0.3
6,359 + +0.7
6,359 + -2.0
6,359 + +0.3
6,360 - +0.6
6,360 - +0.0
6,360 - +0.6
6,360 - -0.6
6,360 - -0.1
6,360 - -2.7
6,360 - +0.5

Or see this region's nucleotide sequence