Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5863 and Pf1N1B4_5864 are separated by 1 nucleotides Pf1N1B4_5864 and Pf1N1B4_5865 are separated by 129 nucleotides
Pf1N1B4_5863 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 297,840 to 298,889
_5863
Pf1N1B4_5864 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 298,891 to 300,108
_5864
Pf1N1B4_5865 - FIG00960493: hypothetical protein, at 300,238 to 301,629
_5865
Position (kb)
298
299
300
301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.306 kb on - strand, within Pf1N1B4_5863 at 298.554 kb on + strand at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.887 kb on + strand at 298.887 kb on + strand at 298.888 kb on - strand at 298.890 kb on + strand at 298.891 kb on - strand at 299.002 kb on + strand at 299.003 kb on - strand at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.048 kb on - strand, within Pf1N1B4_5864 at 299.531 kb on - strand, within Pf1N1B4_5864 at 299.692 kb on + strand, within Pf1N1B4_5864 at 299.693 kb on - strand, within Pf1N1B4_5864 at 299.814 kb on + strand at 299.849 kb on + strand, within Pf1N1B4_5864 at 299.929 kb on + strand at 300.060 kb on + strand at 300.061 kb on - strand at 300.328 kb on + strand at 300.532 kb on + strand, within Pf1N1B4_5865 at 300.533 kb on - strand, within Pf1N1B4_5865 at 300.766 kb on + strand at 300.767 kb on - strand, within Pf1N1B4_5865 at 300.909 kb on + strand, within Pf1N1B4_5865
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 298,003 - Pf1N1B4_5863 0.16 -1.6 298,003 - Pf1N1B4_5863 0.16 +0.4 298,306 - Pf1N1B4_5863 0.44 -0.4 298,554 + +2.9 298,583 - Pf1N1B4_5863 0.71 -2.6 298,583 - Pf1N1B4_5863 0.71 +0.2 298,887 + -0.7 298,887 + +1.4 298,888 - +1.2 298,890 + -0.7 298,891 - -0.9 299,002 + +0.3 299,003 - -0.1 299,047 + Pf1N1B4_5864 0.13 -1.1 299,047 + Pf1N1B4_5864 0.13 -0.8 299,048 - Pf1N1B4_5864 0.13 -0.5 299,531 - Pf1N1B4_5864 0.53 -1.5 299,692 + Pf1N1B4_5864 0.66 -0.1 299,693 - Pf1N1B4_5864 0.66 -0.8 299,814 + -0.8 299,849 + Pf1N1B4_5864 0.79 +0.1 299,929 + +0.7 300,060 + +2.1 300,061 - +1.3 300,328 + -1.2 300,532 + Pf1N1B4_5865 0.21 +2.0 300,533 - Pf1N1B4_5865 0.21 -0.0 300,766 + +1.1 300,767 - Pf1N1B4_5865 0.38 -0.3 300,909 + Pf1N1B4_5865 0.48 -1.1
Or see this region's nucleotide sequence