Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5853 and Pf1N1B4_5854 overlap by 17 nucleotides Pf1N1B4_5854 and Pf1N1B4_5855 are separated by 57 nucleotides
Pf1N1B4_5853 - Glutamine synthetase (EC 6.3.1.2), putative, at 285,255 to 286,586
_5853
Pf1N1B4_5854 - FIG00960144: hypothetical protein, at 286,570 to 287,322
_5854
Pf1N1B4_5855 - Putrescine importer, at 287,380 to 288,729
_5855
Position (kb) 286
287
288 Strain fitness (log2 ratio) -2
-1
0
1 at 286.005 kb on + strand, within Pf1N1B4_5853 at 286.005 kb on + strand, within Pf1N1B4_5853 at 286.071 kb on + strand, within Pf1N1B4_5853 at 286.116 kb on + strand, within Pf1N1B4_5853 at 286.116 kb on + strand, within Pf1N1B4_5853 at 286.240 kb on - strand, within Pf1N1B4_5853 at 286.570 kb on - strand at 286.607 kb on - strand at 286.624 kb on - strand at 286.819 kb on + strand at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.820 kb on - strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.092 kb on + strand, within Pf1N1B4_5854 at 287.093 kb on - strand, within Pf1N1B4_5854 at 287.173 kb on + strand, within Pf1N1B4_5854 at 287.302 kb on + strand at 287.320 kb on + strand at 287.328 kb on + strand at 287.329 kb on - strand at 287.329 kb on - strand at 287.387 kb on + strand at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.537 kb on - strand, within Pf1N1B4_5855 at 287.620 kb on + strand, within Pf1N1B4_5855 at 287.620 kb on + strand, within Pf1N1B4_5855 at 287.621 kb on - strand at 287.661 kb on + strand, within Pf1N1B4_5855 at 287.690 kb on - strand, within Pf1N1B4_5855 at 287.820 kb on + strand, within Pf1N1B4_5855 at 287.821 kb on - strand, within Pf1N1B4_5855 at 287.852 kb on + strand, within Pf1N1B4_5855 at 287.891 kb on - strand, within Pf1N1B4_5855 at 287.922 kb on + strand, within Pf1N1B4_5855 at 287.950 kb on + strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 288.194 kb on - strand, within Pf1N1B4_5855
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 286,005 + Pf1N1B4_5853 0.56 +1.7 286,005 + Pf1N1B4_5853 0.56 -1.3 286,071 + Pf1N1B4_5853 0.61 -0.8 286,116 + Pf1N1B4_5853 0.65 +0.4 286,116 + Pf1N1B4_5853 0.65 +0.4 286,240 - Pf1N1B4_5853 0.74 +0.8 286,570 - +0.4 286,607 - +1.7 286,624 - +0.5 286,819 + -0.2 286,819 + Pf1N1B4_5854 0.33 -0.1 286,819 + Pf1N1B4_5854 0.33 -0.8 286,820 - Pf1N1B4_5854 0.33 +1.1 287,051 + Pf1N1B4_5854 0.64 +0.4 287,051 + Pf1N1B4_5854 0.64 +0.7 287,053 + Pf1N1B4_5854 0.64 +0.5 287,053 + Pf1N1B4_5854 0.64 +0.7 287,053 + Pf1N1B4_5854 0.64 +0.3 287,053 + Pf1N1B4_5854 0.64 +1.7 287,053 + Pf1N1B4_5854 0.64 +0.8 287,092 + Pf1N1B4_5854 0.69 +1.4 287,093 - Pf1N1B4_5854 0.69 -0.5 287,173 + Pf1N1B4_5854 0.80 +0.7 287,302 + -0.2 287,320 + +0.5 287,328 + -0.1 287,329 - +0.9 287,329 - +0.7 287,387 + -0.1 287,536 + Pf1N1B4_5855 0.12 -0.1 287,536 + Pf1N1B4_5855 0.12 +1.2 287,537 - Pf1N1B4_5855 0.12 +0.7 287,620 + Pf1N1B4_5855 0.18 -2.1 287,620 + Pf1N1B4_5855 0.18 +0.9 287,621 - -1.5 287,661 + Pf1N1B4_5855 0.21 -1.2 287,690 - Pf1N1B4_5855 0.23 +0.1 287,820 + Pf1N1B4_5855 0.33 -2.3 287,821 - Pf1N1B4_5855 0.33 -1.8 287,852 + Pf1N1B4_5855 0.35 -0.5 287,891 - Pf1N1B4_5855 0.38 -0.3 287,922 + Pf1N1B4_5855 0.40 +0.0 287,950 + Pf1N1B4_5855 0.42 -0.1 287,951 - Pf1N1B4_5855 0.42 +0.9 287,951 - Pf1N1B4_5855 0.42 -0.1 287,951 - Pf1N1B4_5855 0.42 -1.1 288,194 - Pf1N1B4_5855 0.60 -0.6
Or see this region's nucleotide sequence