Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5782 and Pf1N1B4_5783 are separated by 76 nucleotides Pf1N1B4_5783 and Pf1N1B4_5784 are separated by 22 nucleotides Pf1N1B4_5784 and Pf1N1B4_5785 overlap by 28 nucleotides
Pf1N1B4_5782 - Nitrous oxide reductase maturation protein NosR, at 219,839 to 222,052
_5782
Pf1N1B4_5783 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 222,129 to 223,517
_5783
Pf1N1B4_5784 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 223,540 to 224,085
_5784
Pf1N1B4_5785 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 224,058 to 224,423
_5785
Position (kb) 222
223
224 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 221.244 kb on + strand, within Pf1N1B4_5782 at 221.245 kb on - strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.684 kb on + strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.689 kb on - strand, within Pf1N1B4_5782 at 222.056 kb on - strand at 222.493 kb on + strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.655 kb on + strand, within Pf1N1B4_5783 at 222.776 kb on - strand, within Pf1N1B4_5783 at 222.781 kb on + strand at 223.156 kb on + strand at 223.307 kb on - strand, within Pf1N1B4_5783 at 223.307 kb on - strand, within Pf1N1B4_5783 at 223.309 kb on + strand at 223.309 kb on + strand at 223.342 kb on + strand, within Pf1N1B4_5783 at 223.342 kb on + strand at 223.342 kb on + strand, within Pf1N1B4_5783 at 223.451 kb on + strand at 223.451 kb on + strand at 223.452 kb on - strand at 223.452 kb on - strand at 223.453 kb on + strand at 223.475 kb on - strand at 223.883 kb on + strand, within Pf1N1B4_5784 at 223.883 kb on + strand, within Pf1N1B4_5784 at 223.883 kb on + strand, within Pf1N1B4_5784 at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.040 kb on - strand at 224.165 kb on - strand, within Pf1N1B4_5785 at 224.165 kb on - strand at 224.335 kb on + strand, within Pf1N1B4_5785 at 224.464 kb on + strand at 224.464 kb on + strand at 224.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 221,244 + Pf1N1B4_5782 0.63 +2.5 221,245 - +0.5 221,684 + Pf1N1B4_5782 0.83 +3.9 221,684 + -0.8 221,684 + Pf1N1B4_5782 0.83 -2.2 221,688 + Pf1N1B4_5782 0.84 -1.3 221,688 + Pf1N1B4_5782 0.84 +0.9 221,688 + Pf1N1B4_5782 0.84 -1.8 221,688 + Pf1N1B4_5782 0.84 -1.0 221,688 + Pf1N1B4_5782 0.84 -0.4 221,688 + Pf1N1B4_5782 0.84 +0.5 221,688 + Pf1N1B4_5782 0.84 -2.7 221,688 + Pf1N1B4_5782 0.84 +0.6 221,689 - Pf1N1B4_5782 0.84 +0.4 222,056 - +0.0 222,493 + Pf1N1B4_5783 0.26 -1.5 222,494 - Pf1N1B4_5783 0.26 -2.1 222,494 - Pf1N1B4_5783 0.26 -1.7 222,649 + Pf1N1B4_5783 0.37 -1.1 222,649 + Pf1N1B4_5783 0.37 +0.1 222,650 - Pf1N1B4_5783 0.38 -2.8 222,650 - Pf1N1B4_5783 0.38 -0.1 222,650 - Pf1N1B4_5783 0.38 -0.1 222,655 + Pf1N1B4_5783 0.38 -1.3 222,776 - Pf1N1B4_5783 0.47 -0.9 222,781 + +0.5 223,156 + -0.8 223,307 - Pf1N1B4_5783 0.85 +0.5 223,307 - Pf1N1B4_5783 0.85 -0.0 223,309 + -0.3 223,309 + +0.5 223,342 + Pf1N1B4_5783 0.87 -0.7 223,342 + -0.5 223,342 + Pf1N1B4_5783 0.87 -0.8 223,451 + -0.1 223,451 + -0.2 223,452 - +1.3 223,452 - -0.3 223,453 + -1.2 223,475 - -1.2 223,883 + Pf1N1B4_5784 0.63 -0.5 223,883 + Pf1N1B4_5784 0.63 +0.6 223,883 + Pf1N1B4_5784 0.63 -0.2 224,039 + +0.8 224,039 + -2.1 224,039 + +1.5 224,039 + -0.1 224,040 - -1.5 224,165 - Pf1N1B4_5785 0.29 -2.0 224,165 - +0.5 224,335 + Pf1N1B4_5785 0.76 +0.1 224,464 + -0.2 224,464 + +0.9 224,465 - -0.1
Or see this region's nucleotide sequence