Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5778 and Pf1N1B4_5779 overlap by 4 nucleotides Pf1N1B4_5779 and Pf1N1B4_5780 overlap by 4 nucleotides Pf1N1B4_5780 and Pf1N1B4_5781 are separated by 27 nucleotides
Pf1N1B4_5778 - Nitrous oxide reductase maturation transmembrane protein NosY, at 214,797 to 215,627
_5778
Pf1N1B4_5779 - Nitrous oxide reductase maturation protein NosF (ATPase), at 215,624 to 216,547
_5779
Pf1N1B4_5780 - Nitrous oxide reductase maturation protein NosD, at 216,544 to 217,836
_5780
Pf1N1B4_5781 - Nitrous-oxide reductase (EC 1.7.99.6), at 217,864 to 219,783
_5781
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 215.668 kb on - strand at 215.668 kb on - strand at 215.676 kb on + strand at 215.735 kb on + strand, within Pf1N1B4_5779 at 215.736 kb on - strand, within Pf1N1B4_5779 at 215.987 kb on + strand, within Pf1N1B4_5779 at 215.987 kb on + strand at 215.988 kb on - strand, within Pf1N1B4_5779 at 215.988 kb on - strand at 216.291 kb on + strand, within Pf1N1B4_5779 at 216.505 kb on - strand at 216.510 kb on + strand at 216.738 kb on - strand at 216.842 kb on + strand at 216.843 kb on - strand, within Pf1N1B4_5780 at 216.975 kb on - strand, within Pf1N1B4_5780 at 217.070 kb on + strand, within Pf1N1B4_5780 at 217.079 kb on + strand, within Pf1N1B4_5780 at 217.080 kb on - strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.221 kb on - strand at 217.289 kb on + strand, within Pf1N1B4_5780 at 217.289 kb on + strand, within Pf1N1B4_5780 at 217.349 kb on + strand, within Pf1N1B4_5780 at 217.563 kb on + strand, within Pf1N1B4_5780 at 217.934 kb on + strand at 217.934 kb on + strand at 217.972 kb on - strand at 217.972 kb on - strand at 217.972 kb on - strand at 218.118 kb on - strand, within Pf1N1B4_5781 at 218.141 kb on + strand, within Pf1N1B4_5781 at 218.141 kb on + strand, within Pf1N1B4_5781 at 218.142 kb on - strand at 218.496 kb on - strand, within Pf1N1B4_5781 at 218.522 kb on + strand at 218.522 kb on + strand, within Pf1N1B4_5781 at 218.544 kb on - strand, within Pf1N1B4_5781 at 218.544 kb on - strand, within Pf1N1B4_5781 at 218.774 kb on + strand, within Pf1N1B4_5781 at 218.774 kb on + strand, within Pf1N1B4_5781 at 218.774 kb on + strand at 218.774 kb on + strand, within Pf1N1B4_5781 at 218.775 kb on - strand, within Pf1N1B4_5781 at 218.775 kb on - strand, within Pf1N1B4_5781 at 218.775 kb on - strand, within Pf1N1B4_5781 at 218.829 kb on - strand, within Pf1N1B4_5781
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 215,668 - -0.8 215,668 - -2.3 215,676 + -0.4 215,735 + Pf1N1B4_5779 0.12 -1.3 215,736 - Pf1N1B4_5779 0.12 +0.5 215,987 + Pf1N1B4_5779 0.39 +1.5 215,987 + -0.1 215,988 - Pf1N1B4_5779 0.39 -0.7 215,988 - -1.5 216,291 + Pf1N1B4_5779 0.72 -2.2 216,505 - +0.5 216,510 + -0.5 216,738 - -0.5 216,842 + +0.5 216,843 - Pf1N1B4_5780 0.23 +0.5 216,975 - Pf1N1B4_5780 0.33 +0.5 217,070 + Pf1N1B4_5780 0.41 -1.5 217,079 + Pf1N1B4_5780 0.41 -1.8 217,080 - Pf1N1B4_5780 0.41 -1.5 217,220 + Pf1N1B4_5780 0.52 -2.0 217,220 + Pf1N1B4_5780 0.52 -1.1 217,220 + Pf1N1B4_5780 0.52 -2.5 217,220 + Pf1N1B4_5780 0.52 -1.1 217,220 + Pf1N1B4_5780 0.52 -2.0 217,220 + Pf1N1B4_5780 0.52 -1.3 217,221 - -1.7 217,289 + Pf1N1B4_5780 0.58 +0.5 217,289 + Pf1N1B4_5780 0.58 +0.1 217,349 + Pf1N1B4_5780 0.62 -0.8 217,563 + Pf1N1B4_5780 0.79 -1.7 217,934 + +2.5 217,934 + +2.8 217,972 - +0.3 217,972 - -2.9 217,972 - -0.7 218,118 - Pf1N1B4_5781 0.13 -0.7 218,141 + Pf1N1B4_5781 0.14 -3.0 218,141 + Pf1N1B4_5781 0.14 -0.9 218,142 - -0.8 218,496 - Pf1N1B4_5781 0.33 -2.0 218,522 + -1.8 218,522 + Pf1N1B4_5781 0.34 -0.5 218,544 - Pf1N1B4_5781 0.35 -0.6 218,544 - Pf1N1B4_5781 0.35 -0.2 218,774 + Pf1N1B4_5781 0.47 +0.5 218,774 + Pf1N1B4_5781 0.47 -2.0 218,774 + +1.5 218,774 + Pf1N1B4_5781 0.47 -0.7 218,775 - Pf1N1B4_5781 0.47 +0.3 218,775 - Pf1N1B4_5781 0.47 +0.9 218,775 - Pf1N1B4_5781 0.47 -0.5 218,829 - Pf1N1B4_5781 0.50 +1.2
Or see this region's nucleotide sequence