Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5776 and Pf1N1B4_5777 are separated by 115 nucleotides Pf1N1B4_5777 and Pf1N1B4_5778 are separated by 31 nucleotides Pf1N1B4_5778 and Pf1N1B4_5779 overlap by 4 nucleotides Pf1N1B4_5779 and Pf1N1B4_5780 overlap by 4 nucleotides
Pf1N1B4_5776 - short-chain dehydrogenase/reductase, at 213,379 to 214,122
_5776
Pf1N1B4_5777 - Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL, at 214,238 to 214,765
_5777
Pf1N1B4_5778 - Nitrous oxide reductase maturation transmembrane protein NosY, at 214,797 to 215,627
_5778
Pf1N1B4_5779 - Nitrous oxide reductase maturation protein NosF (ATPase), at 215,624 to 216,547
_5779
Pf1N1B4_5780 - Nitrous oxide reductase maturation protein NosD, at 216,544 to 217,836
_5780
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 213.876 kb on - strand, within Pf1N1B4_5776 at 214.175 kb on + strand at 214.175 kb on + strand at 214.197 kb on + strand at 214.198 kb on - strand at 214.370 kb on + strand, within Pf1N1B4_5777 at 214.410 kb on + strand at 214.410 kb on + strand, within Pf1N1B4_5777 at 214.411 kb on - strand, within Pf1N1B4_5777 at 214.485 kb on + strand at 214.485 kb on + strand, within Pf1N1B4_5777 at 214.486 kb on - strand, within Pf1N1B4_5777 at 214.718 kb on + strand at 214.719 kb on - strand at 214.760 kb on - strand at 214.760 kb on - strand at 214.986 kb on + strand, within Pf1N1B4_5778 at 215.143 kb on + strand, within Pf1N1B4_5778 at 215.668 kb on - strand at 215.668 kb on - strand at 215.676 kb on + strand at 215.735 kb on + strand, within Pf1N1B4_5779 at 215.736 kb on - strand, within Pf1N1B4_5779 at 215.987 kb on + strand, within Pf1N1B4_5779 at 215.987 kb on + strand at 215.988 kb on - strand, within Pf1N1B4_5779 at 215.988 kb on - strand at 216.291 kb on + strand, within Pf1N1B4_5779 at 216.505 kb on - strand at 216.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 213,876 - Pf1N1B4_5776 0.67 +0.5 214,175 + -1.4 214,175 + -0.4 214,197 + -2.0 214,198 - -0.8 214,370 + Pf1N1B4_5777 0.25 -2.3 214,410 + -1.1 214,410 + Pf1N1B4_5777 0.33 -0.2 214,411 - Pf1N1B4_5777 0.33 -1.2 214,485 + -1.7 214,485 + Pf1N1B4_5777 0.47 -0.6 214,486 - Pf1N1B4_5777 0.47 -1.2 214,718 + -0.2 214,719 - +0.5 214,760 - -2.2 214,760 - -1.3 214,986 + Pf1N1B4_5778 0.23 -1.1 215,143 + Pf1N1B4_5778 0.42 -0.5 215,668 - -0.8 215,668 - -2.3 215,676 + -0.4 215,735 + Pf1N1B4_5779 0.12 -1.3 215,736 - Pf1N1B4_5779 0.12 +0.5 215,987 + Pf1N1B4_5779 0.39 +1.5 215,987 + -0.1 215,988 - Pf1N1B4_5779 0.39 -0.7 215,988 - -1.5 216,291 + Pf1N1B4_5779 0.72 -2.2 216,505 - +0.5 216,510 + -0.5
Or see this region's nucleotide sequence