Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5773

Experiment: Nitrate reduction, replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5772 and Pf1N1B4_5773 are separated by 70 nucleotidesPf1N1B4_5773 and Pf1N1B4_5774 overlap by 4 nucleotides Pf1N1B4_5772 - Heme d1 biosynthesis protein NirJ, at 208,778 to 209,956 _5772 Pf1N1B4_5773 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 210,027 to 210,866 _5773 Pf1N1B4_5774 - Nitrite reductase associated c-type cytochorome NirN, at 210,863 to 212,356 _5774 Position (kb) 210 211Strain fitness (log2 ratio) -2 -1 0 1at 209.166 kb on - strand, within Pf1N1B4_5772at 209.373 kb on - strand, within Pf1N1B4_5772at 209.373 kb on - strand, within Pf1N1B4_5772at 209.391 kb on - strand, within Pf1N1B4_5772at 209.681 kb on + strand, within Pf1N1B4_5772at 209.681 kb on + strand, within Pf1N1B4_5772at 209.681 kb on + strand, within Pf1N1B4_5772at 210.378 kb on + strand, within Pf1N1B4_5773at 210.579 kb on + strand, within Pf1N1B4_5773at 210.580 kb on - strand, within Pf1N1B4_5773at 211.228 kb on + strand, within Pf1N1B4_5774at 211.229 kb on - strand, within Pf1N1B4_5774at 211.406 kb on + strand, within Pf1N1B4_5774at 211.439 kb on + strand, within Pf1N1B4_5774at 211.439 kb on + strand, within Pf1N1B4_5774at 211.542 kb on - strandat 211.542 kb on - strand, within Pf1N1B4_5774at 211.542 kb on - strand, within Pf1N1B4_5774at 211.793 kb on + strand, within Pf1N1B4_5774

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C
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209,166 - Pf1N1B4_5772 0.33 -1.5
209,373 - Pf1N1B4_5772 0.50 -0.8
209,373 - Pf1N1B4_5772 0.50 +0.7
209,391 - Pf1N1B4_5772 0.52 -1.5
209,681 + Pf1N1B4_5772 0.77 -1.5
209,681 + Pf1N1B4_5772 0.77 -1.4
209,681 + Pf1N1B4_5772 0.77 -2.5
210,378 + Pf1N1B4_5773 0.42 -0.1
210,579 + Pf1N1B4_5773 0.66 -2.5
210,580 - Pf1N1B4_5773 0.66 -1.4
211,228 + Pf1N1B4_5774 0.24 +0.3
211,229 - Pf1N1B4_5774 0.24 -0.1
211,406 + Pf1N1B4_5774 0.36 +0.5
211,439 + Pf1N1B4_5774 0.39 -1.5
211,439 + Pf1N1B4_5774 0.39 +0.1
211,542 - -2.4
211,542 - Pf1N1B4_5774 0.45 -0.6
211,542 - Pf1N1B4_5774 0.45 -0.5
211,793 + Pf1N1B4_5774 0.62 -2.2

Or see this region's nucleotide sequence