Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5759 and Pf1N1B4_5760 are separated by 3 nucleotides Pf1N1B4_5760 and Pf1N1B4_5761 are separated by 79 nucleotides Pf1N1B4_5761 and Pf1N1B4_5762 are separated by 11 nucleotides Pf1N1B4_5762 and Pf1N1B4_5763 are separated by 43 nucleotides
Pf1N1B4_5759 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 199,183 to 200,610
_5759
Pf1N1B4_5760 - Nitric-oxide reductase subunit C (EC 1.7.99.7), at 200,614 to 201,222
_5760
Pf1N1B4_5761 - Nitric oxide reductase activation protein NorF, at 201,302 to 201,559
_5761
Pf1N1B4_5762 - Nitric oxide reductase activation protein NorE, at 201,571 to 202,098
_5762
Pf1N1B4_5763 - Nitric oxide reductase activation protein NorQ, at 202,142 to 202,933
_5763
Position (kb) 201
202
203 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 200.615 kb on + strand at 200.616 kb on - strand at 200.640 kb on + strand at 200.641 kb on - strand at 200.641 kb on - strand at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 201.015 kb on - strand at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.018 kb on + strand, within Pf1N1B4_5760 at 201.087 kb on + strand, within Pf1N1B4_5760 at 201.330 kb on - strand, within Pf1N1B4_5761 at 201.633 kb on - strand, within Pf1N1B4_5762 at 201.730 kb on + strand, within Pf1N1B4_5762 at 201.730 kb on + strand, within Pf1N1B4_5762 at 201.731 kb on - strand, within Pf1N1B4_5762 at 201.846 kb on - strand, within Pf1N1B4_5762 at 201.878 kb on + strand, within Pf1N1B4_5762 at 201.878 kb on + strand, within Pf1N1B4_5762 at 201.879 kb on - strand, within Pf1N1B4_5762 at 202.270 kb on + strand, within Pf1N1B4_5763 at 202.501 kb on - strand, within Pf1N1B4_5763 at 202.764 kb on + strand, within Pf1N1B4_5763 at 202.902 kb on + strand at 202.902 kb on + strand at 202.903 kb on - strand at 202.903 kb on - strand at 203.053 kb on - strand at 203.053 kb on - strand at 203.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 200,615 + -1.9 200,616 - -1.2 200,640 + -1.0 200,641 - -2.6 200,641 - -0.5 200,770 + Pf1N1B4_5760 0.26 -1.3 200,770 + Pf1N1B4_5760 0.26 -0.8 200,770 + Pf1N1B4_5760 0.26 +0.2 201,015 - -0.3 201,015 - Pf1N1B4_5760 0.66 -2.3 201,015 - Pf1N1B4_5760 0.66 -1.7 201,015 - Pf1N1B4_5760 0.66 -1.4 201,018 + Pf1N1B4_5760 0.66 +0.2 201,087 + Pf1N1B4_5760 0.78 +0.7 201,330 - Pf1N1B4_5761 0.11 -1.5 201,633 - Pf1N1B4_5762 0.12 -0.4 201,730 + Pf1N1B4_5762 0.30 -1.2 201,730 + Pf1N1B4_5762 0.30 -1.1 201,731 - Pf1N1B4_5762 0.30 +2.0 201,846 - Pf1N1B4_5762 0.52 +0.0 201,878 + Pf1N1B4_5762 0.58 +0.0 201,878 + Pf1N1B4_5762 0.58 +0.2 201,879 - Pf1N1B4_5762 0.58 -0.5 202,270 + Pf1N1B4_5763 0.16 -1.6 202,501 - Pf1N1B4_5763 0.45 -3.0 202,764 + Pf1N1B4_5763 0.79 -3.1 202,902 + -1.4 202,902 + -2.3 202,903 - -1.7 202,903 - -2.2 203,053 - -2.2 203,053 - +1.2 203,069 - -2.1
Or see this region's nucleotide sequence