Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5752 and Pf1N1B4_5753 are separated by 75 nucleotides Pf1N1B4_5753 and Pf1N1B4_5754 are separated by 28 nucleotides Pf1N1B4_5754 and Pf1N1B4_5755 overlap by 7 nucleotides
Pf1N1B4_5752 - Nitric oxide-dependent regulator DnrN or NorA, at 193,195 to 193,869
_5752
Pf1N1B4_5753 - putative membrane protein, at 193,945 to 194,382
_5753
Pf1N1B4_5754 - NnrS protein involved in response to NO, at 194,411 to 195,073
_5754
Pf1N1B4_5755 - NnrS protein involved in response to NO, at 195,067 to 195,600
_5755
Position (kb) 194
195
196 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 193.755 kb on + strand, within Pf1N1B4_5752 at 193.942 kb on + strand at 194.079 kb on - strand, within Pf1N1B4_5753 at 194.079 kb on - strand, within Pf1N1B4_5753 at 194.199 kb on - strand, within Pf1N1B4_5753 at 194.272 kb on + strand at 194.470 kb on - strand at 194.911 kb on - strand, within Pf1N1B4_5754 at 194.912 kb on + strand, within Pf1N1B4_5754 at 194.913 kb on - strand, within Pf1N1B4_5754 at 195.171 kb on + strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.615 kb on - strand at 195.655 kb on + strand at 195.656 kb on - strand at 195.656 kb on - strand at 195.926 kb on + strand at 195.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 193,755 + Pf1N1B4_5752 0.83 -0.1 193,942 + -2.1 194,079 - Pf1N1B4_5753 0.31 +0.5 194,079 - Pf1N1B4_5753 0.31 +0.5 194,199 - Pf1N1B4_5753 0.58 -0.1 194,272 + +1.5 194,470 - +1.1 194,911 - Pf1N1B4_5754 0.75 -1.7 194,912 + Pf1N1B4_5754 0.76 -2.7 194,913 - Pf1N1B4_5754 0.76 -4.1 195,171 + Pf1N1B4_5755 0.19 -0.4 195,172 - Pf1N1B4_5755 0.20 +0.5 195,172 - Pf1N1B4_5755 0.20 -2.3 195,172 - Pf1N1B4_5755 0.20 -0.5 195,172 - Pf1N1B4_5755 0.20 -0.4 195,615 - -0.1 195,655 + -2.2 195,656 - -0.1 195,656 - -0.6 195,926 + +0.6 195,927 - -1.0
Or see this region's nucleotide sequence