Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5740 and Pf1N1B4_5741 overlap by 4 nucleotides Pf1N1B4_5741 and Pf1N1B4_5742 are separated by 86 nucleotides Pf1N1B4_5742 and Pf1N1B4_5743 are separated by 74 nucleotides
Pf1N1B4_5740 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 181,651 to 183,453
_5740
Pf1N1B4_5741 - Nitrate/nitrite response regulator protein, at 183,450 to 184,097
_5741
Pf1N1B4_5742 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 184,184 to 184,858
_5742
Pf1N1B4_5743 - hypothetical protein, at 184,933 to 185,331
_5743
Position (kb) 184
185 Strain fitness (log2 ratio) -2
-1
0
1
2 at 183.222 kb on - strand, within Pf1N1B4_5740 at 183.805 kb on - strand, within Pf1N1B4_5741 at 184.134 kb on + strand at 184.134 kb on + strand at 184.134 kb on + strand at 184.160 kb on + strand at 184.160 kb on + strand at 184.161 kb on - strand at 184.179 kb on - strand at 184.210 kb on - strand at 184.510 kb on - strand, within Pf1N1B4_5742 at 184.961 kb on - strand at 185.306 kb on + strand at 185.391 kb on + strand at 185.391 kb on + strand at 185.393 kb on + strand at 185.408 kb on + strand at 185.724 kb on + strand at 185.796 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 183,222 - Pf1N1B4_5740 0.87 -2.7 183,805 - Pf1N1B4_5741 0.55 -1.0 184,134 + +0.6 184,134 + +0.8 184,134 + -1.5 184,160 + +0.5 184,160 + -0.0 184,161 - -0.8 184,179 - -1.4 184,210 - -0.5 184,510 - Pf1N1B4_5742 0.48 +2.1 184,961 - -0.1 185,306 + -0.7 185,391 + -0.7 185,391 + +0.6 185,393 + +0.3 185,408 + -0.7 185,724 + -0.6 185,796 - +0.4
Or see this region's nucleotide sequence