Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5716 and Pf1N1B4_5717 are separated by 159 nucleotides Pf1N1B4_5717 and Pf1N1B4_5718 are separated by 100 nucleotides Pf1N1B4_5718 and Pf1N1B4_5719 are separated by 354 nucleotides
Pf1N1B4_5716 - Acriflavin resistance protein, at 157,751 to 160,846
_5716
Pf1N1B4_5717 - hypothetical protein, at 161,006 to 161,122
_5717
Pf1N1B4_5718 - Transcriptional regulator, Cro/CI family, at 161,223 to 162,014
_5718
Pf1N1B4_5719 - hypothetical protein, at 162,369 to 163,043
_5719
Position (kb) 161
162
163 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 160.369 kb on + strand, within Pf1N1B4_5716 at 160.369 kb on + strand, within Pf1N1B4_5716 at 160.369 kb on + strand, within Pf1N1B4_5716 at 160.369 kb on + strand at 160.369 kb on + strand, within Pf1N1B4_5716 at 160.369 kb on + strand, within Pf1N1B4_5716 at 160.370 kb on - strand, within Pf1N1B4_5716 at 160.370 kb on - strand, within Pf1N1B4_5716 at 160.370 kb on - strand, within Pf1N1B4_5716 at 160.370 kb on - strand, within Pf1N1B4_5716 at 160.370 kb on - strand, within Pf1N1B4_5716 at 160.763 kb on + strand at 160.763 kb on + strand at 160.763 kb on + strand at 160.764 kb on - strand at 160.882 kb on + strand at 160.882 kb on + strand at 160.883 kb on - strand at 160.885 kb on + strand at 160.885 kb on + strand at 160.885 kb on + strand at 160.885 kb on + strand at 160.885 kb on + strand at 160.886 kb on - strand at 160.886 kb on - strand at 160.886 kb on - strand at 160.886 kb on - strand at 160.886 kb on - strand at 161.172 kb on - strand at 161.283 kb on - strand at 161.283 kb on - strand at 161.287 kb on - strand at 161.530 kb on + strand, within Pf1N1B4_5718 at 161.530 kb on + strand at 161.589 kb on + strand, within Pf1N1B4_5718 at 161.589 kb on + strand, within Pf1N1B4_5718 at 161.696 kb on + strand, within Pf1N1B4_5718 at 161.832 kb on + strand, within Pf1N1B4_5718 at 161.832 kb on + strand, within Pf1N1B4_5718 at 161.833 kb on - strand at 161.937 kb on + strand at 161.937 kb on + strand at 161.937 kb on + strand at 161.938 kb on - strand at 162.053 kb on + strand at 162.131 kb on - strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.298 kb on + strand at 162.298 kb on + strand at 162.304 kb on + strand at 162.305 kb on - strand at 162.305 kb on - strand at 162.355 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.391 kb on - strand at 162.413 kb on + strand at 162.413 kb on + strand at 162.759 kb on + strand, within Pf1N1B4_5719 at 162.760 kb on - strand, within Pf1N1B4_5719 at 162.876 kb on - strand, within Pf1N1B4_5719 at 162.957 kb on + strand, within Pf1N1B4_5719 at 162.958 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 160,369 + Pf1N1B4_5716 0.85 +2.2 160,369 + Pf1N1B4_5716 0.85 -0.7 160,369 + Pf1N1B4_5716 0.85 -0.5 160,369 + +0.7 160,369 + Pf1N1B4_5716 0.85 -1.7 160,369 + Pf1N1B4_5716 0.85 -0.6 160,370 - Pf1N1B4_5716 0.85 +1.3 160,370 - Pf1N1B4_5716 0.85 -2.1 160,370 - Pf1N1B4_5716 0.85 +0.8 160,370 - Pf1N1B4_5716 0.85 +0.2 160,370 - Pf1N1B4_5716 0.85 -1.4 160,763 + -2.0 160,763 + -1.7 160,763 + -0.9 160,764 - -1.0 160,882 + -1.6 160,882 + +2.5 160,883 - -0.5 160,885 + -0.5 160,885 + -2.1 160,885 + +0.1 160,885 + -0.5 160,885 + -0.2 160,886 - -0.5 160,886 - -1.1 160,886 - -0.4 160,886 - -1.5 160,886 - -0.1 161,172 - +0.2 161,283 - -2.3 161,283 - -1.4 161,287 - -1.3 161,530 + Pf1N1B4_5718 0.39 +1.6 161,530 + +0.5 161,589 + Pf1N1B4_5718 0.46 +1.0 161,589 + Pf1N1B4_5718 0.46 +1.1 161,696 + Pf1N1B4_5718 0.60 -0.1 161,832 + Pf1N1B4_5718 0.77 -0.7 161,832 + Pf1N1B4_5718 0.77 -1.1 161,833 - -0.1 161,937 + +0.2 161,937 + +0.5 161,937 + -2.3 161,938 - +0.3 162,053 + +0.8 162,131 - +0.6 162,141 + -0.6 162,141 + +0.5 162,141 + -1.6 162,190 + +0.0 162,190 + -2.0 162,190 + +0.2 162,298 + -1.1 162,298 + -4.5 162,304 + -1.1 162,305 - +0.3 162,305 - +0.8 162,355 + -0.3 162,390 + +0.3 162,390 + +1.5 162,390 + -0.1 162,391 - +1.7 162,413 + -0.5 162,413 + +0.5 162,759 + Pf1N1B4_5719 0.58 +0.4 162,760 - Pf1N1B4_5719 0.58 -0.5 162,876 - Pf1N1B4_5719 0.75 -0.8 162,957 + Pf1N1B4_5719 0.87 -2.8 162,958 - -0.6
Or see this region's nucleotide sequence