Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5423 and Pf1N1B4_5424 are separated by 5 nucleotides Pf1N1B4_5424 and Pf1N1B4_5425 are separated by 543 nucleotides Pf1N1B4_5425 and Pf1N1B4_5426 are separated by 461 nucleotides Pf1N1B4_5426 and Pf1N1B4_5427 are separated by 130 nucleotides
Pf1N1B4_5423 - RecD-like DNA helicase YrrC, at 162,581 to 164,764
_5423
Pf1N1B4_5424 - hypothetical protein, at 164,770 to 164,952
_5424
Pf1N1B4_5425 - DNA-binding protein HU-beta, at 165,496 to 165,771
_5425
Pf1N1B4_5426 - hypothetical protein, at 166,233 to 166,580
_5426
Pf1N1B4_5427 - C4-type zinc finger protein, DksA/TraR family, at 166,711 to 167,184
_5427
Position (kb) 165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 164.499 kb on + strand, within Pf1N1B4_5423 at 164.500 kb on - strand, within Pf1N1B4_5423 at 164.500 kb on - strand, within Pf1N1B4_5423 at 164.533 kb on + strand, within Pf1N1B4_5423 at 164.533 kb on + strand, within Pf1N1B4_5423 at 164.740 kb on + strand at 164.839 kb on - strand, within Pf1N1B4_5424 at 164.839 kb on - strand at 164.895 kb on - strand, within Pf1N1B4_5424 at 164.895 kb on - strand, within Pf1N1B4_5424 at 165.160 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.269 kb on + strand at 165.276 kb on + strand at 165.276 kb on + strand at 165.276 kb on + strand at 165.276 kb on + strand at 165.279 kb on + strand at 165.279 kb on + strand at 165.280 kb on - strand at 165.337 kb on + strand at 165.419 kb on + strand at 165.419 kb on + strand at 165.535 kb on - strand, within Pf1N1B4_5425 at 165.640 kb on + strand, within Pf1N1B4_5425 at 165.641 kb on - strand, within Pf1N1B4_5425 at 165.641 kb on - strand, within Pf1N1B4_5425 at 165.724 kb on + strand, within Pf1N1B4_5425 at 165.725 kb on - strand, within Pf1N1B4_5425 at 165.828 kb on - strand at 165.848 kb on + strand at 165.849 kb on - strand at 165.903 kb on - strand at 165.949 kb on + strand at 165.950 kb on - strand at 165.950 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.128 kb on - strand at 166.211 kb on - strand at 166.222 kb on + strand at 166.222 kb on + strand at 166.223 kb on - strand at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.347 kb on - strand, within Pf1N1B4_5426 at 166.371 kb on + strand, within Pf1N1B4_5426 at 166.372 kb on - strand, within Pf1N1B4_5426 at 166.439 kb on - strand, within Pf1N1B4_5426 at 166.532 kb on - strand, within Pf1N1B4_5426 at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.604 kb on - strand at 166.604 kb on - strand at 166.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 164,499 + Pf1N1B4_5423 0.88 -0.8 164,500 - Pf1N1B4_5423 0.88 +2.5 164,500 - Pf1N1B4_5423 0.88 -0.0 164,533 + Pf1N1B4_5423 0.89 -0.7 164,533 + Pf1N1B4_5423 0.89 -1.0 164,740 + -0.7 164,839 - Pf1N1B4_5424 0.38 -2.0 164,839 - -0.5 164,895 - Pf1N1B4_5424 0.68 +0.5 164,895 - Pf1N1B4_5424 0.68 -0.1 165,160 + +0.5 165,244 + -0.3 165,244 + -1.0 165,244 + +0.2 165,244 + +0.5 165,244 + -0.1 165,269 + -0.5 165,276 + -1.1 165,276 + -0.4 165,276 + +0.7 165,276 + -0.3 165,279 + +0.1 165,279 + -1.3 165,280 - -0.7 165,337 + -0.5 165,419 + -1.7 165,419 + +0.1 165,535 - Pf1N1B4_5425 0.14 -0.5 165,640 + Pf1N1B4_5425 0.52 -2.9 165,641 - Pf1N1B4_5425 0.53 -0.9 165,641 - Pf1N1B4_5425 0.53 +0.1 165,724 + Pf1N1B4_5425 0.83 -1.7 165,725 - Pf1N1B4_5425 0.83 -2.3 165,828 - -0.8 165,848 + -1.3 165,849 - -0.4 165,903 - -1.5 165,949 + +1.0 165,950 - +0.3 165,950 - +2.0 166,031 - -0.5 166,031 - -0.9 166,031 - -1.0 166,128 - -1.1 166,211 - -0.3 166,222 + -0.4 166,222 + +0.8 166,223 - -0.7 166,274 + Pf1N1B4_5426 0.12 -1.1 166,274 + Pf1N1B4_5426 0.12 -1.3 166,274 + Pf1N1B4_5426 0.12 -0.0 166,275 - Pf1N1B4_5426 0.12 -0.4 166,275 - Pf1N1B4_5426 0.12 +0.3 166,287 + Pf1N1B4_5426 0.16 -0.6 166,287 + Pf1N1B4_5426 0.16 -0.8 166,287 + Pf1N1B4_5426 0.16 -0.3 166,287 + Pf1N1B4_5426 0.16 -1.1 166,287 + Pf1N1B4_5426 0.16 -0.9 166,288 - Pf1N1B4_5426 0.16 +0.3 166,288 - Pf1N1B4_5426 0.16 -0.4 166,288 - Pf1N1B4_5426 0.16 -0.2 166,347 - Pf1N1B4_5426 0.33 -0.7 166,371 + Pf1N1B4_5426 0.40 -0.5 166,372 - Pf1N1B4_5426 0.40 +1.0 166,439 - Pf1N1B4_5426 0.59 -0.1 166,532 - Pf1N1B4_5426 0.86 -0.5 166,603 + +1.5 166,603 + -1.0 166,603 + +0.1 166,603 + -0.7 166,603 + -0.4 166,604 - +3.0 166,604 - -0.2 166,604 - -2.1
Or see this region's nucleotide sequence