Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5378 and Pf1N1B4_5379 are separated by 756 nucleotides Pf1N1B4_5379 and Pf1N1B4_5380 are separated by 125 nucleotides Pf1N1B4_5380 and Pf1N1B4_5381 are separated by 266 nucleotides
Pf1N1B4_5378 - Phage protein, at 111,089 to 112,282
_5378
Pf1N1B4_5379 - hypothetical protein, at 113,039 to 113,362
_5379
Pf1N1B4_5380 - hypothetical protein, at 113,488 to 113,916
_5380
Pf1N1B4_5381 - hypothetical protein, at 114,183 to 114,464
_5381
Position (kb)
113
114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand, within Pf1N1B4_5378 at 112.088 kb on + strand at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.089 kb on - strand, within Pf1N1B4_5378 at 112.332 kb on + strand at 112.332 kb on + strand at 112.333 kb on - strand at 112.333 kb on - strand at 112.333 kb on - strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.347 kb on + strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.348 kb on - strand at 112.386 kb on - strand at 112.427 kb on + strand at 112.427 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.431 kb on + strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.432 kb on - strand at 112.570 kb on + strand at 112.570 kb on + strand at 112.570 kb on + strand at 112.571 kb on - strand at 112.571 kb on - strand at 112.636 kb on + strand at 112.636 kb on + strand at 112.649 kb on + strand at 112.649 kb on + strand at 112.650 kb on - strand at 112.650 kb on - strand at 112.650 kb on - strand at 112.686 kb on + strand at 112.686 kb on + strand at 112.686 kb on + strand at 112.687 kb on - strand at 112.687 kb on - strand at 112.843 kb on + strand at 112.870 kb on + strand at 112.871 kb on - strand at 112.871 kb on - strand at 112.903 kb on + strand at 112.903 kb on + strand at 112.903 kb on + strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.904 kb on - strand at 112.912 kb on + strand at 112.913 kb on - strand at 112.913 kb on - strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.038 kb on + strand at 113.039 kb on - strand at 113.133 kb on + strand, within Pf1N1B4_5379 at 113.156 kb on + strand, within Pf1N1B4_5379 at 113.360 kb on + strand at 113.360 kb on + strand at 113.361 kb on - strand at 113.372 kb on + strand at 113.407 kb on + strand at 113.428 kb on - strand at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand, within Pf1N1B4_5380 at 113.629 kb on + strand at 113.630 kb on - strand, within Pf1N1B4_5380 at 113.632 kb on + strand, within Pf1N1B4_5380 at 113.632 kb on + strand, within Pf1N1B4_5380 at 113.633 kb on - strand, within Pf1N1B4_5380 at 113.633 kb on - strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.659 kb on + strand, within Pf1N1B4_5380 at 113.660 kb on - strand at 113.682 kb on + strand, within Pf1N1B4_5380 at 113.721 kb on + strand, within Pf1N1B4_5380 at 113.758 kb on + strand, within Pf1N1B4_5380 at 113.944 kb on + strand at 113.944 kb on + strand at 113.944 kb on + strand at 113.944 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.012 kb on + strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.013 kb on - strand at 114.035 kb on + strand at 114.101 kb on + strand at 114.109 kb on - strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.143 kb on + strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.144 kb on - strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.147 kb on + strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.148 kb on - strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.164 kb on - strand at 114.181 kb on + strand at 114.181 kb on + strand at 114.281 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.286 kb on + strand, within Pf1N1B4_5381 at 114.320 kb on + strand, within Pf1N1B4_5381 at 114.321 kb on - strand, within Pf1N1B4_5381 at 114.343 kb on + strand at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381 at 114.343 kb on + strand, within Pf1N1B4_5381
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 112,088 + Pf1N1B4_5378 0.84 -1.2 112,088 + Pf1N1B4_5378 0.84 +1.5 112,088 + Pf1N1B4_5378 0.84 -0.1 112,088 + Pf1N1B4_5378 0.84 +1.7 112,088 + Pf1N1B4_5378 0.84 -2.3 112,088 + -1.3 112,089 - Pf1N1B4_5378 0.84 +0.5 112,089 - Pf1N1B4_5378 0.84 +1.7 112,089 - Pf1N1B4_5378 0.84 +1.3 112,089 - Pf1N1B4_5378 0.84 -1.4 112,089 - Pf1N1B4_5378 0.84 +2.4 112,332 + -1.9 112,332 + +2.0 112,333 - -0.5 112,333 - -1.5 112,333 - -0.5 112,347 + -0.2 112,347 + -0.5 112,347 + +3.1 112,347 + -1.0 112,348 - +0.5 112,348 - +0.1 112,348 - -0.5 112,348 - -1.1 112,348 - -1.1 112,348 - -0.2 112,348 - -1.0 112,348 - +1.2 112,386 - +0.1 112,427 + -0.6 112,427 + -1.9 112,431 + +0.3 112,431 + -1.1 112,431 + -0.1 112,431 + -0.5 112,431 + -0.2 112,431 + +0.9 112,431 + -0.3 112,432 - -0.4 112,432 - +0.5 112,432 - -0.1 112,432 - -2.1 112,432 - +0.2 112,432 - +0.7 112,570 + +0.5 112,570 + -0.9 112,570 + -0.2 112,571 - -0.9 112,571 - +0.5 112,636 + -0.9 112,636 + -0.5 112,649 + +1.1 112,649 + -1.5 112,650 - -0.5 112,650 - +0.3 112,650 - +0.1 112,686 + +1.1 112,686 + +0.1 112,686 + -0.3 112,687 - -0.3 112,687 - +2.8 112,843 + +1.3 112,870 + +1.0 112,871 - +0.9 112,871 - +0.2 112,903 + -1.1 112,903 + -1.4 112,903 + -0.6 112,904 - +1.9 112,904 - -0.3 112,904 - +0.4 112,904 - -0.3 112,904 - -3.2 112,912 + +1.1 112,913 - +0.2 112,913 - -1.3 113,038 + +0.7 113,038 + -2.1 113,038 + +0.4 113,038 + -1.5 113,039 - -0.9 113,133 + Pf1N1B4_5379 0.29 +1.5 113,156 + Pf1N1B4_5379 0.36 -0.5 113,360 + -1.0 113,360 + +3.2 113,361 - +1.1 113,372 + +0.5 113,407 + -2.0 113,428 - -0.5 113,629 + Pf1N1B4_5380 0.33 -0.1 113,629 + Pf1N1B4_5380 0.33 +0.4 113,629 + Pf1N1B4_5380 0.33 +0.5 113,629 + +0.1 113,630 - Pf1N1B4_5380 0.33 +0.9 113,632 + Pf1N1B4_5380 0.34 +0.1 113,632 + Pf1N1B4_5380 0.34 -1.5 113,633 - Pf1N1B4_5380 0.34 +1.6 113,633 - Pf1N1B4_5380 0.34 -1.4 113,659 + Pf1N1B4_5380 0.40 -0.1 113,659 + Pf1N1B4_5380 0.40 +0.3 113,659 + Pf1N1B4_5380 0.40 -0.6 113,659 + Pf1N1B4_5380 0.40 +1.5 113,660 - +1.1 113,682 + Pf1N1B4_5380 0.45 +0.2 113,721 + Pf1N1B4_5380 0.54 -0.3 113,758 + Pf1N1B4_5380 0.63 -0.5 113,944 + +0.5 113,944 + -1.8 113,944 + -1.1 113,944 + +0.9 114,012 + -0.6 114,012 + +1.0 114,012 + -2.3 114,012 + +0.3 114,012 + -1.9 114,012 + +0.9 114,012 + -0.6 114,013 - -0.1 114,013 - +0.1 114,013 - +0.5 114,035 + -0.9 114,101 + -1.9 114,109 - +2.5 114,143 + +0.5 114,143 + +1.5 114,143 + -0.3 114,143 + -0.1 114,143 + +0.1 114,143 + -3.1 114,143 + +0.1 114,143 + -0.1 114,143 + +0.5 114,143 + -0.6 114,143 + -0.0 114,143 + -0.9 114,143 + +1.0 114,143 + +0.1 114,143 + +0.5 114,143 + -0.5 114,143 + -1.1 114,144 - +0.1 114,144 - -1.5 114,144 - -0.3 114,147 + -0.6 114,147 + +0.6 114,147 + +1.5 114,147 + +0.1 114,147 + -0.9 114,147 + -1.1 114,147 + -2.0 114,147 + +2.1 114,147 + -0.1 114,147 + -1.1 114,147 + +1.7 114,148 - +0.2 114,148 - -0.5 114,148 - -0.7 114,148 - +0.1 114,163 + +0.9 114,163 + -0.8 114,163 + -0.1 114,164 - +0.5 114,181 + -1.1 114,181 + -0.6 114,281 + Pf1N1B4_5381 0.35 -1.6 114,286 + Pf1N1B4_5381 0.37 +1.5 114,286 + Pf1N1B4_5381 0.37 -1.5 114,320 + Pf1N1B4_5381 0.49 -0.5 114,321 - Pf1N1B4_5381 0.49 -3.0 114,343 + -1.3 114,343 + Pf1N1B4_5381 0.57 +0.2 114,343 + Pf1N1B4_5381 0.57 -0.9 114,343 + Pf1N1B4_5381 0.57 +0.9 114,343 + Pf1N1B4_5381 0.57 +0.5 114,343 + Pf1N1B4_5381 0.57 +0.5 114,343 + Pf1N1B4_5381 0.57 -0.5 114,343 + Pf1N1B4_5381 0.57 -0.3 114,343 + Pf1N1B4_5381 0.57 -0.5
Or see this region's nucleotide sequence