Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5279

Experiment: Nitrate reduction, replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5277 and Pf1N1B4_5278 are separated by 155 nucleotidesPf1N1B4_5278 and Pf1N1B4_5279 are separated by 320 nucleotidesPf1N1B4_5279 and Pf1N1B4_5280 are separated by 160 nucleotides Pf1N1B4_5277 - Phage protein, at 33,051 to 33,326 _5277 Pf1N1B4_5278 - hypothetical protein, at 33,482 to 33,682 _5278 Pf1N1B4_5279 - hypothetical protein, at 34,003 to 34,209 _5279 Pf1N1B4_5280 - Nucleoid-associated protein, at 34,370 to 35,386 _5280 Position (kb) 34 35Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 33.084 kb on + strandat 33.084 kb on + strand, within Pf1N1B4_5277at 33.085 kb on - strand, within Pf1N1B4_5277at 33.085 kb on - strand, within Pf1N1B4_5277at 33.188 kb on + strand, within Pf1N1B4_5277at 33.235 kb on + strand, within Pf1N1B4_5277at 33.236 kb on - strand, within Pf1N1B4_5277at 33.236 kb on - strand, within Pf1N1B4_5277at 33.263 kb on + strand, within Pf1N1B4_5277at 33.263 kb on + strand, within Pf1N1B4_5277at 33.264 kb on - strand, within Pf1N1B4_5277at 33.266 kb on + strand, within Pf1N1B4_5277at 33.266 kb on + strand, within Pf1N1B4_5277at 33.267 kb on - strand, within Pf1N1B4_5277at 33.361 kb on - strandat 33.361 kb on - strandat 33.484 kb on - strandat 33.484 kb on - strandat 33.627 kb on - strand, within Pf1N1B4_5278at 33.650 kb on + strandat 33.650 kb on + strandat 33.650 kb on + strand, within Pf1N1B4_5278at 33.651 kb on - strand, within Pf1N1B4_5278at 33.651 kb on - strandat 33.651 kb on - strand, within Pf1N1B4_5278at 33.765 kb on + strandat 33.838 kb on + strandat 33.953 kb on + strandat 33.969 kb on - strandat 33.993 kb on + strandat 33.994 kb on - strandat 34.022 kb on + strandat 34.085 kb on + strand, within Pf1N1B4_5279at 34.102 kb on + strand, within Pf1N1B4_5279at 34.102 kb on + strand, within Pf1N1B4_5279at 34.102 kb on + strand, within Pf1N1B4_5279at 34.102 kb on + strand, within Pf1N1B4_5279at 34.103 kb on - strand, within Pf1N1B4_5279at 34.103 kb on - strandat 34.213 kb on + strandat 34.213 kb on + strandat 34.214 kb on - strandat 34.214 kb on - strandat 34.214 kb on - strandat 34.267 kb on + strandat 34.267 kb on + strandat 34.268 kb on - strandat 34.268 kb on - strandat 34.268 kb on - strandat 34.605 kb on + strand, within Pf1N1B4_5280at 34.606 kb on - strand, within Pf1N1B4_5280at 34.836 kb on + strand, within Pf1N1B4_5280at 34.836 kb on + strand, within Pf1N1B4_5280at 34.837 kb on - strand, within Pf1N1B4_5280at 34.861 kb on + strand, within Pf1N1B4_5280at 34.861 kb on + strand, within Pf1N1B4_5280at 34.862 kb on - strand, within Pf1N1B4_5280at 34.862 kb on - strand, within Pf1N1B4_5280at 34.862 kb on - strand, within Pf1N1B4_5280at 34.881 kb on + strandat 34.881 kb on + strand, within Pf1N1B4_5280at 34.881 kb on + strand, within Pf1N1B4_5280at 34.881 kb on + strand, within Pf1N1B4_5280at 34.881 kb on + strand, within Pf1N1B4_5280at 34.881 kb on + strand, within Pf1N1B4_5280at 34.882 kb on - strand, within Pf1N1B4_5280at 34.882 kb on - strand, within Pf1N1B4_5280at 34.882 kb on - strand, within Pf1N1B4_5280at 35.056 kb on - strand, within Pf1N1B4_5280at 35.170 kb on - strand, within Pf1N1B4_5280at 35.170 kb on - strand, within Pf1N1B4_5280at 35.170 kb on - strand, within Pf1N1B4_5280at 35.170 kb on - strand, within Pf1N1B4_5280

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C
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33,084 + -0.3
33,084 + Pf1N1B4_5277 0.12 +0.0
33,085 - Pf1N1B4_5277 0.12 -0.8
33,085 - Pf1N1B4_5277 0.12 -0.3
33,188 + Pf1N1B4_5277 0.50 -1.9
33,235 + Pf1N1B4_5277 0.67 +2.6
33,236 - Pf1N1B4_5277 0.67 -2.3
33,236 - Pf1N1B4_5277 0.67 +1.2
33,263 + Pf1N1B4_5277 0.77 -0.3
33,263 + Pf1N1B4_5277 0.77 +0.6
33,264 - Pf1N1B4_5277 0.77 -0.5
33,266 + Pf1N1B4_5277 0.78 +2.2
33,266 + Pf1N1B4_5277 0.78 -0.9
33,267 - Pf1N1B4_5277 0.78 +0.1
33,361 - +0.3
33,361 - -0.6
33,484 - -0.1
33,484 - -0.3
33,627 - Pf1N1B4_5278 0.72 -0.1
33,650 + -0.5
33,650 + +2.5
33,650 + Pf1N1B4_5278 0.84 -0.4
33,651 - Pf1N1B4_5278 0.84 -0.2
33,651 - -1.5
33,651 - Pf1N1B4_5278 0.84 +0.0
33,765 + +2.0
33,838 + +0.5
33,953 + -1.7
33,969 - +0.5
33,993 + +1.0
33,994 - -1.1
34,022 + -0.5
34,085 + Pf1N1B4_5279 0.40 +0.4
34,102 + Pf1N1B4_5279 0.48 -0.5
34,102 + Pf1N1B4_5279 0.48 +1.1
34,102 + Pf1N1B4_5279 0.48 +0.9
34,102 + Pf1N1B4_5279 0.48 +0.9
34,103 - Pf1N1B4_5279 0.48 -0.3
34,103 - -1.3
34,213 + -0.3
34,213 + -0.4
34,214 - -0.3
34,214 - +2.9
34,214 - -0.9
34,267 + -1.1
34,267 + +2.2
34,268 - -1.2
34,268 - -0.5
34,268 - -1.2
34,605 + Pf1N1B4_5280 0.23 +0.1
34,606 - Pf1N1B4_5280 0.23 -0.5
34,836 + Pf1N1B4_5280 0.46 +0.9
34,836 + Pf1N1B4_5280 0.46 -0.7
34,837 - Pf1N1B4_5280 0.46 -0.6
34,861 + Pf1N1B4_5280 0.48 +0.5
34,861 + Pf1N1B4_5280 0.48 +0.8
34,862 - Pf1N1B4_5280 0.48 -0.9
34,862 - Pf1N1B4_5280 0.48 -0.9
34,862 - Pf1N1B4_5280 0.48 -1.3
34,881 + +0.7
34,881 + Pf1N1B4_5280 0.50 -0.5
34,881 + Pf1N1B4_5280 0.50 -0.8
34,881 + Pf1N1B4_5280 0.50 +4.6
34,881 + Pf1N1B4_5280 0.50 -0.2
34,881 + Pf1N1B4_5280 0.50 +0.1
34,882 - Pf1N1B4_5280 0.50 +0.9
34,882 - Pf1N1B4_5280 0.50 -1.1
34,882 - Pf1N1B4_5280 0.50 -0.3
35,056 - Pf1N1B4_5280 0.67 -0.3
35,170 - Pf1N1B4_5280 0.79 -1.4
35,170 - Pf1N1B4_5280 0.79 -0.1
35,170 - Pf1N1B4_5280 0.79 -1.3
35,170 - Pf1N1B4_5280 0.79 -1.4

Or see this region's nucleotide sequence