Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5260 and Pf1N1B4_5261 are separated by 280 nucleotides Pf1N1B4_5261 and Pf1N1B4_5262 are separated by 372 nucleotides
Pf1N1B4_5260 - hypothetical protein, at 19,541 to 20,695
_5260
Pf1N1B4_5261 - hypothetical protein, at 20,976 to 21,173
_5261
Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034
_5262
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 20.274 kb on - strand, within Pf1N1B4_5260 at 20.277 kb on + strand at 20.281 kb on + strand, within Pf1N1B4_5260 at 20.285 kb on - strand, within Pf1N1B4_5260 at 20.627 kb on + strand at 20.627 kb on + strand at 20.630 kb on + strand at 20.657 kb on + strand at 20.758 kb on + strand at 20.977 kb on + strand at 20.977 kb on + strand at 21.099 kb on + strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.200 kb on + strand at 21.220 kb on + strand at 21.359 kb on + strand at 21.360 kb on - strand at 21.589 kb on + strand at 21.724 kb on + strand, within Pf1N1B4_5262 at 21.725 kb on - strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.943 kb on + strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.961 kb on + strand, within Pf1N1B4_5262 at 22.078 kb on - strand at 22.104 kb on + strand at 22.105 kb on - strand at 22.105 kb on - strand at 22.159 kb on + strand at 22.159 kb on + strand at 22.160 kb on - strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 20,274 - Pf1N1B4_5260 0.63 -0.1 20,277 + +1.5 20,281 + Pf1N1B4_5260 0.64 -0.1 20,285 - Pf1N1B4_5260 0.64 +1.5 20,627 + -1.9 20,627 + -0.8 20,630 + +1.8 20,657 + -0.5 20,758 + +0.9 20,977 + -1.5 20,977 + -0.6 21,099 + Pf1N1B4_5261 0.62 +2.0 21,100 - Pf1N1B4_5261 0.63 -0.3 21,100 - Pf1N1B4_5261 0.63 -0.5 21,100 - Pf1N1B4_5261 0.63 +1.1 21,200 + +0.6 21,220 + -1.3 21,359 + -0.1 21,360 - -1.0 21,589 + +2.0 21,724 + Pf1N1B4_5262 0.36 -0.2 21,725 - Pf1N1B4_5262 0.37 -0.4 21,904 + Pf1N1B4_5262 0.73 -0.9 21,904 + Pf1N1B4_5262 0.73 -0.7 21,904 + Pf1N1B4_5262 0.73 -0.4 21,905 - Pf1N1B4_5262 0.73 -0.5 21,905 - Pf1N1B4_5262 0.73 -0.9 21,905 - Pf1N1B4_5262 0.73 -1.4 21,905 - Pf1N1B4_5262 0.73 +1.5 21,941 + Pf1N1B4_5262 0.81 -3.2 21,941 + Pf1N1B4_5262 0.81 -0.9 21,943 + Pf1N1B4_5262 0.81 +1.3 21,944 - Pf1N1B4_5262 0.81 -0.5 21,944 - Pf1N1B4_5262 0.81 +1.0 21,961 + Pf1N1B4_5262 0.85 -0.1 22,078 - +0.7 22,104 + -0.3 22,105 - -1.5 22,105 - -0.9 22,159 + -0.7 22,159 + -0.5 22,160 - +0.2 22,162 + -1.3 22,162 + -0.9 22,162 + -0.8 22,162 + +1.5 22,162 + +0.3
Or see this region's nucleotide sequence